Human IL-12p40 ELISA Kit (48-well)

CAT#: EA100039

Human IL-12p40 ELISA Kit (48-well)

Size: 1 x 48 wells 1 x 96 wells



CNY 2,890.00


货期*
3周

规格
    • 1 x 48 wells

Product images

Specifications

Product Data
Description Human IL-12p40 ELISA Kit (48-well)
Size 1 x 48 wells
Format 48-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 3 hours
Signal Colorimetric
Curve Range 62.5-4000pg/ml
Sample Type Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume 20 uL
Specificity Natural and recombinant Human IL-12p40 Ligand
Sensitivity 30pg/mL
Reactivity Human
Interference No significant interference observed with available related molecules.
Components
  • Aluminium pouches with a Microwell Plate coated with monoclonal antibody to human IL-12p40 (8x6)
  • 2 vials human IL-12p40 Standard lyophilized, 2000 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human IL-12p40 monoclonal antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 3 pieces Adhesive Films
  • package insert
Background

Interleukin-12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T-cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN. and TNF production. IL-12 is a disulfidelinked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL-12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

Gene Symbol IL12A
Standard Curve
Representative standard curve for IL-12p40 ELISA. IL-12p40 was diluted in serial two-fold steps in Sample Diluent.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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