Human IFN-gamma ELISA Kit (96-well)

Specifications

Product Data
Description	Human IFN-gamma ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Curve Range	15.625-1000pg/ml
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Human IFN-? Ligand
Sensitivity	7pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with antibody to human IFN-? (8 x 12) 2 vials human IFN-? Standard lyophilized, 4000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-human IFN-? antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 4 pieces Adhesive Films package insert
Background	Interferon gamma (IFN-.) is a multifunctional protein first observed as an antiviral activity in cultures of Sindbis virus-infected human leukocytes stimulated by PHA . Produced by Tlymphocytes and natural killer (NK) cells, IFN-. .is now known to be both an inhibitor of viral replication and a regulator of numerous immunological functions. Human IFN-.. is reported to be active only on human and non-human primate cells . The biochemistry and biological activities of the interferons have been extensively reviewed . Human IFN-. .is a 143 amino acid residue, 20 or 25 kDa glycoprotein that demonstrates little sequence homology to IFN-.. or -... Naturally occurring IFN-. .is found as either of two molecular weight species, differing in degree of glycosylation. Human IFN-.. apparently exists as a head-to-tail dimer in solution with the C-terminus of one monomer aligned with the N-terminus of the other monomer A receptor for IFN-. has been identified and its gene localized to chromosome 6 Apparently the product of a single gene, the receptor is a single chain 90 kDa glycoprotein that shows a high degree of species-specific binding of IFN-... Functionally, IFN-. .produces a variety of effects. Produced by CD8+, NK, gd, and TH1 T helper cells, IFN-. .has documented antiviral, antiprotozoal and immunomodulatory effects on cell proliferation and apoptosis, as well as the stimulation and repression of a variety of genes he antiprotozoal activity of IFN-. .against Toxoplasma and Chlamydia is believed to result from indoleamine 2,3-dioxygenase activity, an enzyme induced by IFN-. ..The immunomodulatory effects of IFN-.. are extensive and diverse. In monocyte/macrophages, the activities of IFN-. .include: increasing the expression of class I and II MHC antigens; increasing the production of IL-1, platelet-activating factor, H2O2, and pterin; protection of monocytes against LAK cell-mediated lysis; downregulation of IL-8 mRNA expression that is upregulated by IL-2; and, with lipopolysaccharide, induction of NO production.Finally, IFN-..has been shown to upregulate ICAM-1, but not E-Selectin or VCAM-1, expression on endothelial cells.
Gene Symbol	IFNG
Standard Curve	Representative standard curve for IFN-? ELISA. IFN-? was diluted in serial two-fold steps in Sample Diluent.

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