Rat TNF-a ELISA Kit (96-well)

Specifications

Product Data
Description	Rat TNF-a ELISA Kit (96-well)
Size	1 x 96 wells
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hour
Signal	Colorimetric
Curve Range	0.03 - 2.0 ng/mL
Sample Type	Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume	20 uL
Specificity	Natural and recombinant Rat TNF-a Ligand
Sensitivity	15pg/mL
Reactivity	Rat
Interference	No significant interference observed with available related molecules.
Components	Aluminium pouches with a Microwell Plate coated with monoclonal antibody to rat TNF-a (8 x 12) 2 vials rat TNF-a Standard lyophilized, 2000 pg/ml upon reconstitution 2 vials concentrated Biotin-Conjugate anti-rat TNF-a antibody 2 vials Streptavidin-HRP solution 1 bottle Standard /sample Diluent 1 bottle Biotin-Conjugate antibody Diluent 1 bottle Streptavidin-HRP Diluent 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20) 1 vial Substrate Solution 1 vial Stop Solution 4 pieces Adhesive Films package insert
Background	The prototype ligand of theTNFsuperfamily, TNF-./TNFSF1A, is a pleiotropic cytokine that plays a central role in inflammation and apoptosis .TNF-. is produced by activated macrophages and other cell types including T and B cells, NK cells, LAK cells, astrocytes, endothelial cells, smooth muscle cells and some tumor cells . Rat TNF-. cDNA encodes a 235 amino acid (aa) residue type II membrane protein . The 156 aa residue soluble TNF-.is released from the C-terminus of the membrane-anchored TNF-. by TNF-.-converting enzyme (TACE), a matrix metalloprotease . The membrane-anchored form of TNF-. has been shown to have lytic activity and may also have an important role in intercellular communication . The biologically active TNF-. has been shown to exist as a trimer .TNF-. is reported to promote inflammatory cell infiltration by upregulating leukocyte adhesion molecules on endothelial cells, serve as a chemotactic agent for monocytes, and activate phagocyte killing mechanisms . Deficiencies in either TNF-. or its receptors can increase susceptibility to infection by intracellular pathogens . TNF-. may also play a role in lymphoid tissue development. Knockout mice lack splenic B cell follicles and the ability to form germinal centers . Other potential physiological roles for TNF-. and its receptors include regulating the differentiation of hematopoietic stem and progenitor cells .
Gene Symbol	Tnf
Standard Curve	Representative standard curve for TNF-a ELISA.TNF-a was diluted in serial two-fold steps in Sample Diluent.

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