Rat IL-6 ELISA Kit

Specifications

Product Data
Description	Rat IL-6 ELISA Kit
Size	1 x 96 wells
Assay Type	Sandwich ELISA kit of Quantitative Detection for Rat IL-6
Assay Length	3.5 hours incubations; 1 hour washing and analyzing samples
Signal	Colorimetric
Curve Range	62.5 pg/ml-4000 pg/ml
Sample Type	ell culture supernates, serum and plasma(heparin, EDTA, citrate).
Sample Volume	100 ul
Specificity	This kit is used for quantitative detection of rat IL-6
Sensitivity	< 5 pg/ml
Reactivity	Rat
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	96-well plate precoated with anti- rat IL-6 antibody 1 Lyophilized recombinant rat IL-6 standard 10 ng/tube x 2 Biotinylated anti- rat IL-6 antibody 100 ul(dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC) 100 ul(dilution 1:100) Sample diluent buffer 30 ml Antibody diluent buffer 12 ml ABC diluent buffer 12 ml TMB color developing agent 10 ml TMB stop solution 10 ml Plate seaeler 4 Piece Wash Buffer (25x) 20 ml
Background	Interleukin-6(IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6(IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone(CRH). Interleukin-6(IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor(IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.
Gene Symbol	Il6
Standard Curve	Representative standard curve for Rat IL-6 PicoKine ELISA Kit

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