Mouse CD40L ELISA Kit

CAT#: EA100395

Mouse CD40L ELISA Kit



CNY 5,330.00


货期*
3周

规格
    • 1 x 96 wells

Product images

Specifications

Product Data
Description Mouse CD40L ELISA Kit
Size 1 x 96 wells
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse CD40L
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 62.5pg/ml-4000pg/ml
Sample Type cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse CD40L
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse CD40L antibody 1
  • Lyophilized recombinant mouse CD40L standard 4 ng/tubex2
  • Biotinylated anti- mouse CD40L antibody 100ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC) 100ul(dilution 1:100)
  • Sample diluent buffer 30 ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
  • Plate Sealers 4 x
  • Wash Buffer (25x) 20ml
Background CD40 ligand(CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.
Gene Symbol Cd40lg
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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