Human Cadherin-2/N-Cadherin ELISA Kit

CAT#: EA100427

Human Cadherin-2/N-Cadherin ELISA Kit



CNY 5,000.00


货期*
3周

规格
    • 1 x 96 wells

Product images

Specifications

Product Data
Description Human Cadherin-2/N-Cadherin ELISA Kit
Size 1 x 96 wells
Assay Type Sandwich ELISA kit of Quantitative Detection for Human Cadherin-2/N-Cadherin
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 156 pg/ml - 10.000 pg/ml
Sample Type Cell culture supernates, serum and plasma(heparin, EDTA).
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of human Cadherin-2
Sensitivity < 20 pg/ml
Reactivity Human
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti-human CDH2 antibody | 1
  • Lyophilized recombinant human CDH2 standard | 50 ng/tube x 2
  • Biotinylated anti-human CDH2 antibody | 100 ul (dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) | 100 ul (dilution 1:100)
  • Sample diluent buffer | 30 ml
  • Antibody diluent buffer | 12 ml
  • ABC diluent buffer | 12 ml
  • TMB color developing agent | 10 ml
  • TMB stop solution | 10 ml
  • Adhesive cover | 4
  • Wash Buffer (25x) | 20 ml
Background Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
Gene Symbol CDH2
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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