Mouse Lipocalin-2/NGAL ELISA Kit

CAT#: EA100541

Mouse Lipocalin-2/NGAL ELISA Kit



CNY 5,340.00


货期*
3周

规格
    • 1 x 96 wells

Product images

Specifications

Product Data
Description Mouse Lipocalin-2/NGAL ELISA Kit
Size 1 x 96 wells
Assay Type Sandwich ELISA kit of Quantitative Detection for Mouse Lipocalin-2/NGAL
Assay Length 3.5 hours incubations; 1 hour washing and analyzing samples
Signal Colorimetric
Curve Range 78pg/ml-5,000pg/ml
Sample Type Cell culture supernates, serum, plasma(heparin) and urine.
Sample Volume 100 ul
Specificity This kit is used for quantitative detection of mouse NGAL
Sensitivity <10pg/ml
Reactivity Mouse
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.
Interference No significant interference observed with available related molecules.
Components
  • 96-well plate precoated with anti- mouse Lipocalin-2/NGAL antibody 1
  • Lyophilized recombinant mouse Lipocalin-2/NGAL standard 10ng/tubex2
  • Biotinylated anti- mouse Lipocalin-2/NGAL antibody 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC) 130ul(dilution 1:100)
  • Sample diluent buffer 30ml
  • Antibody diluent buffer 12ml
  • ABC diluent buffer 12ml
  • TMB color developing agent 10ml
  • TMB stop solution 10ml
Background Lipocalin-2(LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide(LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant mouse NGAL, consisting of 179 amino acids with the molecular mass of 22KDa.
Gene Symbol Lcn2
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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