Adrenocorticotropic hormone (ACTH) ELISA (Mouse/Rat) kit

Specifications

Product Data
Description	Adrenocorticotropic hormone (ACTH) ELISA (Mouse/Rat) kit
Size	1 x 96 wells
Format	8x12 divisible strips
Assay Type	Solid Phase Sandwich ELISA
Assay Length	3 hours
Signal	Colorimetric
Sample Type	Serum
Specificity	This kit is used for quantitative detection Adrenocorticotropic hormone (ACTH) (Mouse/Rat)
Reactivity	Mouse, Rat
Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.
Interference	No significant interference observed with available related molecules.
Components	Microwells coated with Streptavidin: 6x2x8 ACTH Standard Zero: 1 bottle, Ready to use: 4 mL ACTH Standards: 5 bottles (Lyophilized): 2 mL Controls 1 & 2 (CTRL) (2 Vials): 2 mL Biotinylated ACTH Antibody (Reagent 1): 2.7 mL Enzyme labeled ACTH Antibody (Reagent 2): 2.7 mL TMB Substrate (Reagent B): 15 ml Stop Solution: 20 ml Wash Concentrate (Reagent A): 30mL
Background	Adrenocorticotropic Hormone (ACTH) is a 39-amino acid peptide hormone (MW = 4500) secreted mainly by the anterior pituitary gland. Various types of stress or pain perceived in higher levels of the brain modulate secretion of the hypothalamic neurosecretory hormone, corticotropin releasing hormone (CRH). CRH stimulates pituitary ACTH secretion. The second peptide that modulates ACTH secretion is vasopressin (AVP). AVP secretion is also stimulated by stress and acts synergistically with CRH to increase ACTH secretion in the pituitary portal circulation.

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