Human CX3CL1 ELISA KIT (96 T)

Specifications

Product Data
Description	Human CX3CL1 Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	312-20000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human CX3CL1.
Sensitivity	150pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,20000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Fractalkine, also known as CX3CL1 or neurotactin, is the only member of the CX3C or delta chemokine subfamily. Fractalkine is produced as a transmembrane protein, unlike all other chemokines except CXCL16. The 95-100 kDa type I transmembrane form of Fractalkine can be proteolytically processed to generate a secreted 60-80 kDa soluble form. Soluble Fractalkine has been identified in serum, urine, cerebrospinal, amniotic and synovial fluids. Human Fractalkine shares 60% or 63% aa sequence identity within the entire extracellular domain, and 78% or 85% aa sequence identity within the chemokine domain only, with mouse or rat Fractalkine, respectively. Fractalkine is predominantly expressed by vascular endothelium and smooth muscle, neurons, dendritic cells, and the epithelial linings of the intestine, bronchi, renal proximal tubules, endometrium, fallopian tube, and bile duct. Most Fractalkine expression is induced by inflammatory cytokines such as TNF-α and IFN-γ, but expression on forebrain neurons as well as Leydig and Sertoli testicular cells is also constitutive. Expression by astrocytes, osteoblasts, stratum basal keratinocytes, and fibroblasts has also been reported.
Gene Symbol	CX3CL1

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