Human CXCL5 ELISA KIT (96 T)

Specifications

Product Data
Description	Human CXCL5 Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	15.62-1000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human CXCL5.
Sensitivity	8pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,2000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Epithelial Neutrophil Activating peptide 78 (ENA-78) is a CXC chemokine (CXCL5) that was originally isolated from media conditioned by the growth of a human lung type-II alveolar epithelial cell line (A549) stimulated by IL-1β or TNF-α. The full-length cDNA encodes a 114 amino acid (aa) residue precursor protein with a 36 aa residue signal peptide that is cleaved to generate the 78 aa residue secreted protein. Like other CXC chemokines, ENA-78 is a neutrophil attractant and activator in vitro. Based on cross-desensitization experiments, it has been suggested that ENA-78 activity can be mediated through the IL-8 receptor system. ENA-78 is expressed in human platelets. The expression of ENA-78 has also been detected in neutrophils and monocytes/macrophages following LPS stimulation. In addition, ENA-78 expression is highly inducible in endothelial cells, vascular smooth muscle cells, epithelial cells and pulmonary fibroblasts by proinflammatory cytokines such as IL-1β or TNF-α. Increased ENA-78 expression has also been found to be associated with neutrophil influx in various inflammatory conditions.
Gene Symbol	CXCL5

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