Human CD166 (ALCAM) ELISA KIT (96 T)

CAT#: EA800071

Human ALCAM Immunoassay



CNY 3,200.00


货期*
3周

规格
    • 96 T

Product images

Specifications

Product Data
Description Human ALCAM Immunoassay
Size 96 T
Format 96-well strip plate
Assay Type Solid Phase Sandwich ELISA
Assay Length 4 hours
Signal Colorimetric
Curve Range 62.5-4000pg/ml
Sample Type Serum, plasma, Cell culture supernatant
Sample Volume 100 uL
Specificity This assay recognizes both natural and recombinant human ALCAM.
Sensitivity 30 pg/mL
Reactivity Human
Interference No significant interference observed with available related molecules.
Components
  • Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate
  • Standard - lyophilized,4000 pg/ml upon reconstitution,2 vials
  • Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials
  • Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial
  • Standard /Sample Diluent - 16 ml/vial,1 bottle
  • Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle
  • Streptavidin-HRP Diluent - 16 ml/vial,1 bottle
  • Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle
  • Substrate Solution - 12 ml/vial,1 bottle
  • Stop Solution - 12 ml/vial,1 bottle
  • Plate Cover Seals,4 pieces
Background ALCAM (activated leukocyte cell adhesion molecule), designated CD166, is a 100 110 kDa type I transmembrane glycoprotein and a member of the Ig CAM family within the immunoglobulin superfamily. ALCAM is expressed on thymic epithelium, microvascular endothelium, activated lymphocytes and monocytes, and monocyte derived dendritic cells. Human ALCAM cDNA encodes 583 amino acid (aa), including signal peptide (27 aa), extracellular domain (ECD, 500 aa) with two V type and three C2 type Ig like domains, transmembrane (22 aa) and cytoplasmic (34 aa) domains. Human ALCAM ECD shares 93%, 95% and 96% aa sequence identity with mouse/rat, bovine and porcine/equine ALCAM, respectively. ALCAM mediates low affinity adhesion with itself or the cysteine rich scavenger receptor CD6 to regulate T cell development, immunological synapses (IS), and cell migration through endothelial junctions. ALCAM on thymic epithelia mediates adhesion to CD6 on CD4+CD8+ T cells.
Gene Symbol ALCAM
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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