Human Galectin 3 (LGALS3) ELISA KIT (96 T)

Specifications

Product Data
Description	Human Galectin 3 Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	62.5-4000pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant human Galectins-3.
Sensitivity	31 pg/mL
Reactivity	Human
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,4000 pg/ml upon reconstitution,2 vials Concentrated Biotin-Conjugated antibody(100X) - 120 ul/vial,1 vials Concentrated Streptavidin-HRP solution(100X) - 120 ul/vial,1 vial Standard /Sample Diluent - 16 ml/vial,1 bottle Biotin-Conjugate antibody Diluent - 16 ml/vial,1 bottle Streptavidin-HRP Diluent - 16 ml/vial,1 bottle Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Substrate Solution - 12 ml/vial,1 bottle Stop Solution - 12 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Galectins are a family of carbohydrate-binding proteins with specificity for N-acetyllactosamine (LacNAc)-containing glycoproteins. At least 14 mammalian galectins share structural similarities in their carbohydrate recognition domains (CRD). Most fall within two groups called prototype (one CRD) or tandem-repeat (two CRDs). Galectin-3, also known as Mac-2 and previously as L29, CBP35, or eBP, is the only known Galectin with one CRD and a unique N-terminus. It is often termed a chimeric galectin. Galectins lack classical signal peptides but are present and active both within and outside of the cell. They are involved in cell adhesion, migration, survival, and apoptosis and are often up- or down-regulated in cancer. Human and mouse Galectin-3 share approximately 86% amino acid (aa) sequence identity. The 250 aa, 29-36 kDa human Galectin-3 is widely expressed in various cells, including macrophages, activated T cells, osteoclasts, epithelial cells, tumor cells, and fibroblasts. Galectin-3 expression is up-regulated in macrophages as compared to monocytes or dendritic cells. Increased serum Galectin-3 has been noted in rheumatoid arthritis (also in synovial fluid), Behcet’s disease, and a variety of cancers, especially when they are metastatic. Cleavage of Galectin-3 in tumors is highly indicative of matrix metalloproteinase activity.
Gene Symbol	LGALS3

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