Mouse ELISA KIT (96 T)

Specifications

Product Data
Description	Mouse IgG(Total) ELISA KIT
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	1.25-80ng/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant mouse IgG.
Sensitivity	250pg/mL
Reactivity	Mouse
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,80 ng/ml upon reconstitution,3 vials lyophilized Biotin-Conjugated antibody,1 vial Concentrated Streptavidin-HRP solution,1 vial Wash Buffer Concentrate (20x) - 30 ml/vial,1 bottle Assay Buffer Concentrate (20x) - 20 ml/vial,1 bottle 20X PBS(20x) - 20 ml/vial,1 bottle Substrate Solution - 10.5 ml/vial,1 bottle Stop Solution - 5.5 ml/vial,1 bottle Plate Cover Seals,4 pieces
Background	Immunoglobulin G (IgG) is antibody molecule. Each IgG is composed of four peptide chains — two heavy chains γ and two light chains. IgG antibodies are large molecules of about 150 kDa composed of four peptide chains. It contains two identical class γ heavy chains of about 50 kDa and two identical light chains of about 25 kDa, thus a tetrameric quaternary structure. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape. Each end of the fork contains an identical antigen binding site. The Fc regions of IgGs bear a highly conserved N-glycosylation site. The N-glycans attached to this site are predominantly core-fucosylated diantennary structures of the complex type. In addition, small amounts of these N-glycans also bear bisecting GlcNAc and α-2, 6-linked sialic acid residues. IgG antibodies are involved in predominantly the secondary immune response. The presence of specific IgG, in general, corresponds to maturation of the antibody response. IgG can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by agglutination and immobilization, complement activation (classical pathway), opsonization for phagocytosis, and neutralization of their toxins.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.