Rabbit IFNG ELISA KIT (96 T)

Specifications

Product Data
Description	Rabbit IFN-γ Immunoassay
Size	96 T
Format	96-well strip plate
Assay Type	Solid Phase Sandwich ELISA
Assay Length	4 hours
Signal	Colorimetric
Curve Range	18.75-1200pg/ml
Sample Type	Serum, plasma, Cell culture supernatant
Sample Volume	100 uL
Specificity	This assay recognizes both natural and recombinant Rabbit IFN-?.
Sensitivity	3 pg/mL
Reactivity	Rabbit
Interference	No significant interference observed with available related molecules.
Components	Microwell Plate - antibody coated 96-well Microplate (8 wells ×12 strips),1 plate Standard - lyophilized,1200pg/ml upon reconstitution,2 vials lyophilized, Biotin-Conjugated antibody,2 vials Concentrated Streptavidin-HRP,1 vial Standard /sample Diluent,1 bottle Biotin-Conjugate antibody Diluent,1 bottle Streptavidin-HRP Diluent,1 bottle 20 x Wash Buffer Concentrate,1 bottle Substrate Solution,1 bottle Stop Solution,1 bottle Plate Cover Seals,4 pieces
Background	IFN-γ is a dimerized soluble cytokine that is the only member of the type II class of interferons. It plays key roles in both the innate and adaptive immune response against viral and intracellular bacterial infections and for tumor control. It alters transcription in up to 30 genes producing a variety of physiological and cellular responses. IFN-γ activates the cytotoxic activity of innate immune cells such as macrophages and NK cells. IFN-γ production by NK cells and antigen-presenting cells (APCs) promotes the cell mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increased class I and class II MHC expression, and enhancing peptide antigen presentation . The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form (IFNGR1/IFNGR2)2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2 and Stat1. The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis.
Gene Symbol	IFNG

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