RPA14 (RPA3) (NM_002947) Human 3' UTR Clone

Specifications

Product Data
Product Name	RPA14 (RPA3) (NM_002947) Human 3' UTR Clone
Vector	pMirTarget
Synonyms	REPA3; RP-A p14
ACCN	NM_002947
Insert Size	512 bp
Sequence Data	Insert Sequence (showhide) >SC200287 3’UTR clone of NM_002947 The sequence shown below is from the reference sequence of NM_002947. The complete sequence of this clone may contain minor differences, such as SNPs. Blue=Stop Codon Red=Cloning site GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC TATCCTTTAGGGATTGTGCAACATGATTGATCTTGATGGATTTTCATACGATTGTAAATGAGCTATATT AAAGTCTATTAAAGGAAGCCCTTCTTGTTTGAGGGAGAGATTTCTGTGCTTTCTCATATTTAATTTGCT GTTTTTAAGATATTCCAACCTAGAGTTTTTGATGGAACTGATATATTGACAGTTCTCACCGAAGTCCTT TTATAAAGAATTGCTACTCCAATATATGGTCAGATTAGATGCAAGAATAAAGCAGTTGTCCGAGTCTAA GTTTCTATTTTATTAATAAAAACTAAAATGGTACGTACTATCGGTCATTTCATTTTCATTCTTTTAATC ATGTATTCAAGCACAAACTTGAAATTTCATAGCCATAAGGTCAAGATTTAGACCTACCAAATAAAACCT TGGGCCAGCTGTGTTAAGGATTTGCTCACCTTTTCCCAAACTATACCTTGATAATTATTTCCTTGATAC CCTACTTACAAAATGAAGTAGATGACATT ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
Restriction Sites	SgfI-MluI
OTI Disclaimer	Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Reference Data
RefSeq	NM_002947.5
Synonyms	REPA3; RP-A p14
Summary	As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER), probably through interaction with UNG (PubMed:9765279). Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA (PubMed:19010961). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105).[UniProtKB/Swiss-Prot Function]
Locus ID	6119
MW	19.9

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