Wwtr1 (BC004640) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	TAZ
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC004640 GTCCGGGAGCCGCGGCGGCTGCGCTCGTCTACGTCTTCTCTGTCGCCTCCTCGCGCAGTGGGAGCGCCCG AGGCCGGTTCCGGGGATGTAAGAGGATAAGCCTTCGGCTGCTGGGAATCCGCTCGGGATCTGCCCGGGAC CGGGTTCCAGCTCGTCAGTTCGGGAGGCGCCCAGGCTTGGCTTCCCCGAGTCCCCAGAAAGATGAATCCG TCCTCGGTGCCCCATCCGCTCCCGCCGCCAGGGCAGCAAGTCATCCACGTCACGCAGGACCTGGACACCG ACCTCGAAGCCCTCTTCAACTCTGTCATGAACCCCAAGCCCAGCTCATGGCGGAAAAAGATCCTCCCGGA GTCCTTCTTTAAGGAGCCCGATTCCGGCTCGCACTCGCGCCAATCCAGCACAGACTCATCAGGCGGCCAC CCGGGGCCTCGACTAGCTGGCGGCGCGCAGCACGTCCGCTCGCACTCGTCGCCCGCATCCCTGCAGCTGG GCACCGGTGCGGGAGCCGCTGGAGGCCCTGCACAGCAGCATGCACATCTCCGCCAGCAGTCCTATGACGT GACCGACGAGCTGCCGTTGCCCCCCGGGTGGGAGATGACCTTCACGGCCACTGGCCAGAGATACTTCCTT AATCACATAGAGAAAATCACCACATGGCAAGACCCCAGGAAGGTGATGAATCAGCCTCTGAATCATGTGA ACCTCCACCCGTCCATCACTTCCACCTCGGTGCCACAGAGGTCCATGGCAGTGTCCCAGCCGAATCTCGC AATGAATCACCAACACCAGCAAGTCGTGGCCACTAGCCTGAGTCCACAGAACCACCCGACTCAGAACCAA CCCACAGGGCTCATGAGTGTGCCCAATGCACTGACCACTCAGCAGCAGCAGCAGCAGAAACTGCGGCTTC AGAGGATCCAGATGGAGAGAGAGAGGATTAGGATGCGTCAAGAGGAGCTCATGAGGCAGGAAGCTGCCCT CTGCCGACAGCTCCCCATGGAAACCGAGACCATGGCCCCTGTCAACACGCCTGCCATGAGCACAGATATG AGATCTGTCACCAACAGTAGCTCAGATCCTTTCCTCAATGGAGGGCCCTATCATTCACGGGAGCAGAGCA CAGACAGTGGCCTGGGGTTAGGGTGCTACAGTGTCCCCACAACTCCAGAAGACTTCCTCAGCAACATGGA CGAGATGGATACAGGTGAAAATTCCGGTCAGACACCCATGACCGTCAATCCCCAGCAGACCCGCTTCCCT GATTTCCTGGACTGCCTTCCAGGAACAAATGTTGACCTCGGGACTTTGGAGTCTGAAGATCTGATCCCTC TCTTCAATGATGTAGAGTCTGCTCTGAACAAAAGCGAGCCCTTTCTAACCTGGCTGTAATCACTACTGTT GTAACGTGATGCAGCTGTGAGCTGACGCGCGTCTTGGGCCTTGCGGACCAAGTGATGAGGCAGAGCGGGC CTGCAGCTGCACCACGTTCTGCCTTTGTACTCACACTCCTTGTCCGTGTGGCCACTTAATCATTGCCTGG TGTTGATTCGCAGGAACTTGCGTTACACAGAAATAAATACTCTCTTTTCATTTTCAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC004640
Insert Size	1188 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004640, AAH04640
RefSeq Size	1610 bp
RefSeq ORF	1188 bp
Locus ID	97064
Gene Summary	This gene encodes a binding protein of the 14-3-3 family of proteins that regulate cell cycle progression, differentiation and apoptosis. The encoded protein is a transcriptional co-activator that binds to the PPXY motif present on transcription factors. The gene product contains a WW domain and, in the C-terminus, a conserved PDZ-binding motif. This gene is distinct from the gene encoding tafazzin. Both genes share the gene symbol Taz. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]

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