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Prkaca (NM_008854) Mouse Untagged Clone
CAT＃: MC200197
Prkaca (untagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca), (10ug)
CNY 3,656.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | C; P; Pk; Pkaca; PKCD |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC003238 sequence for NM_008854
CTTGGGCTGAGGCTCCCCCGCGGGCGGGCGCAGAGAGACGCGGGAAGCAGGGGCTGGGCGGGGGTCGTGG CGCCGCAGCCAGCGCAGCCAGCCCCAGGGGCCGCCGCCTCCGCTGCCCAGCGCGCTCCGGGGCCGCCGGC CACCTTAGCACCCGCCGCGTCGCAGCTCCGGGACTGGCCCCGGCCGCGACGCCGCCGCGATGGGCAACGC CGCCGCCGCCAAGAAGGGCAGCGAGCAGGAGAGCGTGAAAGAGTTCCTAGCCAAAGCCAAGGAAGATTTC CTGAAAAAATGGGAGACCCCTTCTCAGAATACAGCCCAGTTGGATCAGTTTGATAGAATCAAGACCCTTG GCACCGGCTCCTTTGGGCGAGTGATGCTGGTGAAGCACAAGGAGAGTGGGAACCACTACGCCATGAAGAT CTTAGACAAGCAGAAGGTGGTGAAGCTAAAGCAGATCGAGCACACTCTGAATGAGAAGCGCATCCTGCAG GCCGTCAACTTCCCGTTCCTGGTCAAACTTGAATTCTCCTTCAAGGACAACTCAAACCTGTACATGGTCA TGGAGTATGTAGCTGGTGGCGAGATGTTCTCCCACCTACGGCGGATTGGAAGGTTCAGCGAGCCCCATGC CCGTTTCTACGCGGCGCAGATCGTCCTGACCTTTGAGTATCTGCACTCCCTGGACCTCATCTACCGGGAC CTGAAGCCCGAGAATCTTCTCATCGACCAGCAGGGCTATATTCAGGTGACAGACTTCGGTTTTGCCAAGC GTGTGAAAGGCCGTACTTGGACCTTGTGTGGGACCCCTGAGTACTTGGCCCCCGAGATTATCCTGAGCAA AGGCTACAACAAGGCTGTGGACTGGTGGGCTCTCGGAGTCCTCATCTACGAGATGGCTGCTGGTTACCCA CCCTTCTTCGCTGACCAGCCTATCCAGATCTATGAGAAAATCGTCTCTGGGAAGGTGCGGTTCCCATCCC ACTTCAGCTCTGACTTGAAGGACCTGCTGCGGAACCTTCTGCAGGTGGATCTCACCAAGCGCTTTGGGAA CCTCAAGAACGGGGTCAATGACATCAAGAACCACAAGTGGTTTGCCACGACTGACTGGATTGCCATCTAT CAGAGAAAGGTGGAAGCTCCCTTCATACCAAAGTTTAAAGGCCCTGGGGACACGAGTAACTTTGACGACT ATGAGGAGGAAGAGATCCGGGTCTCCATCAATGAGAAGTGTGGCAAGGAGTTTACTGAGTTTTAGGGGTG TGCTTGTGCCCCTTGGGTTCTCTTTCATTTTTTCTTTTTCTTTCTATTTTTTTTCCGGTTGGGGGTGGGA GGGTTGGATCGAACAGCCAGAGGGCCCTAGAGTTCCATGCATCTAATTTAACATCCACTCCACACCCCCA GGGTTAAGGAGAGCAGGAAAGCGCTCCAGATACTGGGGAAGGGGCAACATCAGCTGCTCCCCCTCTCCCT TCTTCTCCACCCTTCCCTGCCTGTTTTCAATGAATTTCTTAGCTCCAGCCATACCCAATCTTGCTGGTGT ATCCAGGGGCAGGGTACGGAAAGAGGGCCCCAAATTCAGCCTCCTTCCCGACCCTAGCACTGGATACTAA GGATGAACGAACAGTAACGCCAACCTTCCCTTCCATGCAGCCCTACCTGGAAAGGGAGATTTTATGACCT GTACAGAGGGCTGCTTGCCAGTGGGGTTTTTTTTTTTTTTTCATTTAAATTAAGTTCCACCAGTGCCTCC CACCCTCCAAACTGTCCCACCCTCCCCAAACACCCTCCTCACTCCCTAAATCCATTCTGATGAGACCCGG GTAGCCAACTGACCCTGTCAAGGAAGGAACTGGGCTTGGAATCTCGCCCTGAGCTGCTAGCCTCCCGGCC CCCCTTTCCAGTGGTCTCATGCCAATTGTCCTGTGCATCAGCCCCCTTAAGAAGCCTCCCCCATCCTGGG CGCCTCGCTTCTAGCTTAGCTGTCAGCTGTCCATCACCTCTTGCCGTGCGTCCCCACTCACTGCAACCCC AAGTCTGATTGTGCTTTTTCTCTCAATAGAAAGGTGGGGAGCTGCTGGGGAAATTACCCCATTTATCCCT GTGTTTATCCCTCTCGTAACTTCTCCCAAAAAGGAGGAGCTCTCAGGCCTGGGTGGGGGCCCCGGGTGGA CGAGGGGGTCGTTCAACCTGTGTGCTTCGAAGGATGAGACTTCCTCTTGAACAGTGTGCTGTTGTAAACA TATTTGAAAACTATTACCAATAAAGTTTTGTTTTAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_008854 |
| Insert Size | 1056 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC003238, AAH03238 |
| RefSeq Size | 2292 bp |
| RefSeq ORF | 1056 bp |
| Locus ID | 18747 |
| UniProt ID | P05132 |
| Gene Summary | This gene encodes a member of the serine/threonine protein kinase family. The holoenzyme, protein kinase A (also known as cyclic-AMP dependent protein kinase), mediates cellular response to changes in cyclic-AMP levels. This gene encodes the alpha catalytic subunit of protein kinase A. Protein kinase A-mediated signaling is transduced via phosphorylation of target proteins, and is important for many cellular functions, including mammalian sperm maturation and motility. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. [provided by RefSeq, Apr 2013] Transcript Variant: This variant (1, also known as Calpha1) represents the longer transcript and encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange
,Ding, Y;Li, J;Enterina, JR;Shen, Y;Zhang, I;Tewson, PH;Mo, GC;Zhang, J;Quinn, AM;Hughes, TE;Maysinger, D;Alford, SC;Zhang, Y;Campbell, RE;,
Nat. Methods
,PubMed ID 25622108
[PRKACA]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205322 | Prkaca (tGFP-tagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca) |
CNY 7,088.00 |
|
| MR205322 | Prkaca (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca) |
CNY 5,488.00 |
|
| MR205322L3 | Lenti ORF clone of Prkaca (Myc-DDK-tagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca) |
CNY 5,890.00 |
|
| MR205322L4 | Lenti ORF clone of Prkaca (mGFP-tagged) - Mouse protein kinase, cAMP dependent, catalytic, alpha (Prkaca) |
CNY 5,890.00 |
