Cldn6 (BC005718) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AL024037
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC005718 CTTAGCAGTCTCTTTTGCAGGCTCGGATATCAGGGGTGGCCGAACCGGACTGGACGTCCGCAGACAAAGC TGACCGAGCACTCGCCCCCTCAACCTCATCATGGCCTCTACTGGTCTGCAAATCTTGGGGATCGTCCTGA CCCTGCTTGGCTGGGTCAACGCCCTGGTGTCCTGTGCCCTGCCCATGTGGAAGGTGACCGCCTTCATCGG CAACAGCATCGTCGTGGCCCAGATGGTGTGGGAGGGGCTGTGGATGTCCTGTGTGGTTCAGAGCACTGGC CAGATGCAGTGCAAGGTGTATGACTCACTGTTGGCGCTGCCCCAGGACCTGCAGGCTGCCAGAGCCCTCT GTGTTGTCACCCTCCTCATTGTCCTGCTTGGCCTGCTCGTGTACCTGGCTGGAGCCAAGTGCACTACCTG TGTGGAAGATAGGAACTCCAAGTCTCGTCTGGTGCTCATCTCTGGCATCATCTTTGTCATTTCTGGGGTC CTGACGCTCATTCCTGTCTGCTGGACTGCCCACTCTATCATCCAGGACTTCTACAACCCCTTGGTGGCTG ATGCTCAAAAGCGGGAGCTGGGGGCCTCCCTCTACCTGGGCTGGGCAGCCTCAGGCCTTTTGCTGCTGGG TGGAGGGCTACTATGCTGCGCCTGCTCTTCTGGAGGGACCCAGGGACCCAGACATTACATGGCCTGCTAT TCTACATCTGTCCCACATTCTCGGGGACCCTCCGAATATCCCACCAAGAATTATGTGTGATGCTCACTGG GGAGTGAGGCTCTGCTAGCCAGGGAACCTGAGCTGAGCCATCAAGAAAGGTTACGGAGTAGTTTGGGGGT TGTCTTATTTCTTGTTTTTCTTTTGTACTACTGGGTATTGAACCTGGGGCCCCACGCATTTGGTAGGCAA GCACTCACCAGTGAGTCATGTGGTGCCCTGTCTGTCCCTAGGGTTTGTTCCACCTGTTTTTGAGACAAGC TCTTCTCATGGAGCTCTGATCGACCTCCAGCTCCAGTTTTCCTGCCTCAACCTGAGTCCAAGCTCCACCT CTCTCTCTGCTGCTGGCATTTCTCACTTTTAGCTACTGAGGCTGGGAGGATGGGACACTTGAGTTTCTGC GTCATTATTCATTCTGGGGATCTTCTGTGCAGTGTTCTAAGGCTGCCTGGCCTTTGTTTTTCTCAAAATA TCCTGTCCCAGTCCCAAGAGTTCCTGCTGTGGCCGGAGGTAACTCACAGGACAGACGCACCCCTTCCTGG TCAGACTTCTCTCCAGCCCTGGAAGGGCAGGTGATAAGTTACCAAGGAATTTTCTACCCTGAGACTTCCA ACCTTTGGCCCCTGAATCCCATCGCCAAGGTTACTGTGTACTCTCCCTACAAAAGGTCCCAGCTGTGCTG TAGACCCTCCTCCCCCACACCTTGCACACTTCCCTGCTCACACAATGCTTTACTAAATAAAACATGATTT TTTTTTTTTAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC005718
Insert Size	660 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005718, AAH05718
RefSeq Size	1499 bp
RefSeq ORF	660 bp
Locus ID	54419
Gene Summary	This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is essential for blastocyst formation in preimplantation mouse embryos, and is invloved in and is crucial for the formation and maintenance of the epidermal permeability barrier. This gene is adjacent to another family member Cldn9 on chromosome 17. [provided by RefSeq, Aug 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.