Ctsb (BC006656) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CB
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC006656 CGGAGCTGCGCGGGTACTTAGGAGTGCACGGGAGAGGCCAGGAGCGGCTCCGTTTGGCTTGGTGGCTGCA GGCCCAGGCTGTCGCGCTGCGCTCGGTGAGTGCAGGATCCAGCATCCAGGATGTGGTGGTCCTTGATCCT TCTTTCTTGCCTGCTGGCACTGACCAGTGCCCATGACAAGCCTTCCTTCCACCCGCTGTCGGATGACCTG ATTAACTATATCAACAAACAGAATACAACATGGCAGGCTGGACGCAACTTCTACAATGTTGACATAAGCT ATCTGAAGAAGCTGTGTGGCACTGTCCTGGGTGGACCCAAACTGCCAGGAAGGGTTGCGTTCGGTGAGGA CATAGATCTACCTGAAACCTTTGATGCACGGGAACAATGGTCCAACTGCCCGACCATTGGACAGATTAGA GACCAGGGCTCCTGCGGCTCTTGTTGGGCATTTGGGGCAGTGGAAGCCATTTCTGACCGAACCTGCATTC ACACCAATGGCCGAGTCAACGTGGAGGTGTCTGCTGAAGACCTGCTTACTTGCTGTGGTATCCAGTGTGG GGACGGCTGTAATGGTGGCTATCCCTCTGGAGCATGGAGCTTCTGGACAAAAAAAGGCCTGGTTTCAGGT GGAGTCTACAATTCTCATGTAGGCTGCTTACCATACACCATCCCTCCCTGCGAGCACCATGTCAATGGCT CCCGTCCCCCATGCACTGGAGAAGGAGATACTCCCAGGTGCAACAAGAGCTGTGAAGCTGGCTACTCCCC ATCCTACAAAGAGGATAAGCACTTTGGGTACACTTCCTACAGCGTGTCTAACAGTGTGAAGGAGATCATG GCAGAAATCTACAAAAATGGCCCAGTGGAGGGTGCCTTCACTGTGTTTTCTGACTTCTTGACTTACAAAT CAGGAGTATACAAGCATGAAGCCGGTGATATGATGGGTGGCCACGCCATCCGCATCCTGGGCTGGGGAGT AGAGAATGGAGTTCCCTACTGGCTGGCAGCCAACTCTTGGAACCTTGACTGGGGTGATAATGGCTTCTTT AAAATCCTCAGAGGAGAGAACCACTGTGGCATTGAATCAGAAATTGTGGCTGGAATCCCACGCACTGACC AGTACTGGGGAAGATTCTAATCTGCTTTGACTTCATTGTCCAGTCCTTAGGGGCTTTTTCCAAAATTTAG CGGCCTTGGCAGGGAATGAGGTAGACAGGGGAGTCTTTGATTCTTTGAGTTCATCTAACATGCATGAAGC TTTTGGCAAGATTTGGACGACTGGACCAGAGCTGCCTGCCATCAGAGCTACCCTCCAAACACTGTCCTTA CCTGGCCTCCTCCTAGCCCATCCCACGACAGTGATCACAGTCTGCATCCTAGCCTCTCCCTAGACTAGTG CGGTTTGTAGTGCCTGCTGTTGTGGCTCTGCCTGCCATACCTCCCACCCCCATCCCCATCCCAGCATCCG AGGACCCAAGGGCTTTGACTGCAGGACTTCCAAAAGAACGAACACTTCACGAGAGGACAAATGCCACCTC TCAGCTGCATCTTGAAGCTGGTCACTTCTGGGTCTGTGGCAGGCATTCGTCAATGGCCCAGTGCAGTTCT CTGGAGAAAGCTACCTTTTCCCAAGGCATCTGCATCCATCGACATTGGTAATGTGGCCAGCTCTCCTTGG TCCTGTCCTCAGCCGATGCTTTTTCAATAGGATTTTTATGCTTTGTGTACCTCAACCAAGTATGAAGAGC TGTACTGGTTTTATAGATCACCCCCAATTGTATGGCTTAAAACAAGCTACATGGTCATGGTTTGCTGTGT GACCGACAGATAACCACAATGAAACTAGTCTGGAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC006656
Insert Size	1020 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC006656, AAH06656
RefSeq Size	1875 bp
RefSeq ORF	1020 bp
Locus ID	13030
Gene Summary	This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015]

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