Cyp8b1 (BC010974) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC010974 CCACGCGTCCGGACAAGTGGAGCTCAGCCTGCTGGAGCCTAGCCATGACGCTGTGGTGTACAGTGCTAGG AGCCCTGCTCACGGTTGTGGGATGCCTGTGTCTGTCCTTGCTGCTCAGGCACCGCAGGCCCTGGGAGCCA CCTCTGGACAAGGGTTTTGTGCCCTGGCTGGGTCACTCCATGGCTTTCCGGAAGAATATGTTTGAATTCT TGAAGGGGATGCGGGCCAAGCACGGGGATGTCTTCACGGTGCAGCTGGGAGGCCAGTACTTCACCTTTGT CATGGACCCCCTGTCCTTTGGCCCCATCATTAAGAACACAGAGAAAGCGCTGGACTTCCAGTCCTATGCA AAAGAACTGGTGCTCAAGGTGTTTGGGTACCAGTCTGTAGATGGGGACCACAGGATGATCCACTTAGCTA GTACCAAGCATCTGATGGGGCAAGGCTTGGAGGAACTCAACCAGGCCATGCTGGACAGCCTATCCTTGGT GATGCTAGGGCCTAAAGGTTCGAGTCTGGGTGCCAGCTCCTGGTGTGAAGATGGCCTCTTTCACTTCTGC TACAGAATCCTGTTTAAGGCTGGCTTCCTGAGCTTATTCGGCTACACCAAGGACAAGCAGCAAGACCTGG ATGAGGCCGATGAGCTGTTCAGGAAGTTCCGTCGATTTGACTTTCTCTTCCCCAGGTTTGTCTACTCCCT ACTGGGGCCCCGGGAGTGGGTGGAAGTGAGCCAGTTGCAGCGTCTCTTCCACCAGAGACTCTCTGTGGAG CAAAACCTGGAAAAAGACGGCATAAGCTGTTGGTTAGGCTACATGCTTCAGTTTCTGAGGGAGCAAGGAA TAGCTTCATCCATGCAGGACAAATTTAACTTTATGATGCTCTGGGCCTCCCAGGGGAACACGGGACCCAC CTGTTTCTGGGTCCTCTTATTCCTGCTGAAGCACCAGGATGCCATGAAGGCTGTGCGTGAGGAAGCCACC AGGGTCATGGGTAAGGCCAGGCTGGAAGCCAAGAAGTCGTTCACTTTCACTCCCAGTGCCCTGAAACACA CTCCCGTGTTGGACAGCGTGATGGAGGAGAGTCTGCGTCTCTGTGCCACCCCCACCCTCCTCAGGGTGGT ACAGGAGGATTATGTCCTGAAGATGGCCAGCGGGCAAGAGTACCAGATCCGTCGCGGAGATAAGGTGGCT CTCTTCCCCTATCTCTCAGTACACATGGACCCCGACATCCACCCTGAGCCCACAGCCTTCAAGTATGATC GGTTCCTCAACCCGGATGGCACCCGGAAAGTGGACTTCTACAAGTCGGGCAAGAAGATCCACCACTACAG CATGCCTTGGGGCTCGGGTGTTTCCAAGTGCCCCGGCAGGTTCTTTGCCCTCAGCGAGATGAAGACCTTT GTCCTTCTTATGATTATGTACTTCGACTTCAAGCTGGTCGACCCCGACATACCTGTGCCCCCCATTGACC CAAGGCGCTGGGGCTTTGGCACCTCACAGCCTAGCCATGAGGTACGCTTCCTCTATCGCCTGAAGCCCGT GCAGTGAGCTCAGGTCTGAGCATACCCTGCAGCCCAGAGCAGCACTGAATACCCATCCTCTGGCTCTGAG GCACTCCTTCCTCTCCCCAGCTCTCAGAGATCTGTCATGCTCCCCATAAGACGCCATCCCTCCAGCGGTC TCTGCCTGACCCTCCTCTCAGGAGGAACACATGTGGTCAGCTCAGTTTACAACTGATGGCTCACCCACAC TTGACTCATCCTGGAGGGCAGACCAGCTCCCGTGAGTCAGATGGCATCTTGCAAATGCTGCCTCAACCCA TCCCGCCTGCCTTACTCGATGCCCTTACTCCAAATCCTACCAGCTCAGACTCCAGGGATGTTGCTCAATG GAATCGAGCCATGTGTGCGACTGTTAATTTCCCACTAGATATAAAAGCAAGGGATTACGTTGAAAAAAAA AAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC010974
Insert Size	1503 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010974, AAH10974
RefSeq Size	1969 bp
RefSeq ORF	1503 bp
Locus ID	13124
Gene Summary	Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.[UniProtKB/Swiss-Prot Function]

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