Cyp8b1 (BC010974) Mouse Untagged Clone

CAT#: MC200788

Cyp8b1 (untagged) - Mouse cytochrome P450, family 8, subfamily b, polypeptide 1 (cDNA clone MGC:13798 IMAGE:4162964), (10ug)



CNY 3,728.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC010974
CCACGCGTCCGGACAAGTGGAGCTCAGCCTGCTGGAGCCTAGCCATGACGCTGTGGTGTACAGTGCTAGG AGCCCTGCTCACGGTTGTGGGATGCCTGTGTCTGTCCTTGCTGCTCAGGCACCGCAGGCCCTGGGAGCCA CCTCTGGACAAGGGTTTTGTGCCCTGGCTGGGTCACTCCATGGCTTTCCGGAAGAATATGTTTGAATTCT TGAAGGGGATGCGGGCCAAGCACGGGGATGTCTTCACGGTGCAGCTGGGAGGCCAGTACTTCACCTTTGT CATGGACCCCCTGTCCTTTGGCCCCATCATTAAGAACACAGAGAAAGCGCTGGACTTCCAGTCCTATGCA AAAGAACTGGTGCTCAAGGTGTTTGGGTACCAGTCTGTAGATGGGGACCACAGGATGATCCACTTAGCTA GTACCAAGCATCTGATGGGGCAAGGCTTGGAGGAACTCAACCAGGCCATGCTGGACAGCCTATCCTTGGT GATGCTAGGGCCTAAAGGTTCGAGTCTGGGTGCCAGCTCCTGGTGTGAAGATGGCCTCTTTCACTTCTGC TACAGAATCCTGTTTAAGGCTGGCTTCCTGAGCTTATTCGGCTACACCAAGGACAAGCAGCAAGACCTGG ATGAGGCCGATGAGCTGTTCAGGAAGTTCCGTCGATTTGACTTTCTCTTCCCCAGGTTTGTCTACTCCCT ACTGGGGCCCCGGGAGTGGGTGGAAGTGAGCCAGTTGCAGCGTCTCTTCCACCAGAGACTCTCTGTGGAG CAAAACCTGGAAAAAGACGGCATAAGCTGTTGGTTAGGCTACATGCTTCAGTTTCTGAGGGAGCAAGGAA TAGCTTCATCCATGCAGGACAAATTTAACTTTATGATGCTCTGGGCCTCCCAGGGGAACACGGGACCCAC CTGTTTCTGGGTCCTCTTATTCCTGCTGAAGCACCAGGATGCCATGAAGGCTGTGCGTGAGGAAGCCACC AGGGTCATGGGTAAGGCCAGGCTGGAAGCCAAGAAGTCGTTCACTTTCACTCCCAGTGCCCTGAAACACA CTCCCGTGTTGGACAGCGTGATGGAGGAGAGTCTGCGTCTCTGTGCCACCCCCACCCTCCTCAGGGTGGT ACAGGAGGATTATGTCCTGAAGATGGCCAGCGGGCAAGAGTACCAGATCCGTCGCGGAGATAAGGTGGCT CTCTTCCCCTATCTCTCAGTACACATGGACCCCGACATCCACCCTGAGCCCACAGCCTTCAAGTATGATC GGTTCCTCAACCCGGATGGCACCCGGAAAGTGGACTTCTACAAGTCGGGCAAGAAGATCCACCACTACAG CATGCCTTGGGGCTCGGGTGTTTCCAAGTGCCCCGGCAGGTTCTTTGCCCTCAGCGAGATGAAGACCTTT GTCCTTCTTATGATTATGTACTTCGACTTCAAGCTGGTCGACCCCGACATACCTGTGCCCCCCATTGACC CAAGGCGCTGGGGCTTTGGCACCTCACAGCCTAGCCATGAGGTACGCTTCCTCTATCGCCTGAAGCCCGT GCAGTGAGCTCAGGTCTGAGCATACCCTGCAGCCCAGAGCAGCACTGAATACCCATCCTCTGGCTCTGAG GCACTCCTTCCTCTCCCCAGCTCTCAGAGATCTGTCATGCTCCCCATAAGACGCCATCCCTCCAGCGGTC TCTGCCTGACCCTCCTCTCAGGAGGAACACATGTGGTCAGCTCAGTTTACAACTGATGGCTCACCCACAC TTGACTCATCCTGGAGGGCAGACCAGCTCCCGTGAGTCAGATGGCATCTTGCAAATGCTGCCTCAACCCA TCCCGCCTGCCTTACTCGATGCCCTTACTCCAAATCCTACCAGCTCAGACTCCAGGGATGTTGCTCAATG GAATCGAGCCATGTGTGCGACTGTTAATTTCCCACTAGATATAAAAGCAAGGGATTACGTTGAAAAAAAA AAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN BC010974
Insert Size 1503 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC010974, AAH10974
RefSeq Size 1969 bp
RefSeq ORF 1503 bp
Locus ID 13124
Gene Summary Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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