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Ist1 (NM_028018) Mouse Untagged Clone
CAT＃: MC201264
Ist1 (untagged) - Mouse RIKEN cDNA 2400003C14 gene (2400003C14Rik), (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2400003C14Rik; AW536298; mKIAA0174 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC017605 sequence for NM_028018
GAGGGAGTCGCCATTTTGGATGCCGAGCGGCTCCTCGGAAGTCGGCTTCTTCCGTTTTCACGTCCGGATC CCTCCAGGAGGAGCAGGACAACATGCTGGGCTCTGGGTTCAAAGCTGAGCGCTTACGAGTCAATTTGAGA TTAGTTATAAATCGTCTCAAACTGTTGGAGAAAAAAAAAACGGAACTGGCTCAGAAGGCAAGGAAGGAGA TTGCTGACTACCTGGCCGCCGGAAAGGATGAGCGAGCACGCATCCGTGTGGAGCACATAATCCGGGAGGA CTACCTCGTGGAGGCCATGGAGATCCTGGAGCTGTATTGTGACCTGCTGCTGGCGCGCTTTGGCCTTATC CAGTCTATGAAGGAGCTAGATTCTGGTCTAGCTGAATCCGTGTCTACACTGATCTGGGCTGCGCCTCGGC TTCAGTCAGAAGTGGCTGAGTTGAAAATAGTTGCCGATCAGCTCTGTGCCAAATACAGCAAGGAATATGG CAAGTTGTGCAGGACCAACCAGATCGGGACTGTGAATGATAGGCTAATGCACAAACTGAGCGTAGAAGCC CCACCCAAAATCCTAGTGGAGAGATACCTGATTGAAATTGCCAAGAATTACAATGTACCCTACGAACCTG ACTCTGTGGTCATGGCAGAAGCTCCTGTTGGGGTGGAGACAGATCTTATTGATGTTGGATTTACGGACGA TGTGAAGAAAGGTGGTCCTGGAAGAGGAGGAGGGGGCGGGTTCACAGCCCCCGTTGGGGGACCTGATGGG ATTGTGCCCATGCCCATGCCTATGCCCATGCCATCTCCAAATGCTCCCTTCGCATACCCACTGCCAAAGG GACCATCGGATTTCAGTGGATTGCCAGTGGGGACTTACCAGGCCTTTCCTAACATTCACCCACCTCAGAT ACCAGCAACTCCCCCATCCTATGAATCTGTTGATGACATTAATGGTGATAAAACTGTTTCTTCTGCACAG ATTGTTGGACCCAAGCCAGAAGCTCCTGCAAAGCCCCCTTCCAGACCTGTGGATAATTACAACACCTTTG TACTGCCAGAGTTGCCATCGGTGCCAGACACACTTCCAACTGCATCTGCTGGAGCCAGCACCTCAGCATC AGAAGACATAGACTTTGATGATCTTTCCCGGAGATTTGAGGAACTGAAAAAGAAAACATAGGTCTCTTAA ACCAGGCAGCATCCACATTCTGGAAGTTGATAGTGAACAGTTCTCCGTGTAACAGTGACTCTCCATGAAA TTCCGTTTCCTCTGTTAACCGTCACTGAGCACTCTTCCTCGGGGCTCTCTGCTGCTCTACCAATTGTGTT GCTTTCCACTTCTTCAGTGATTCTTCAGTGATCCTGAGAGACTAACAGCTGGAGACTCTAGCTGGAGCAG TTGGCTCCTATTGGCTGTGTTTGCCTGTCTGTCTTCCTGTCACGTGGATCCTTGGTTCTTTCCCGGCCAT TCCATTCCCAGGGAGTGAGAAGTATTGTGGGTAGAGTTGCCAAAGATGGCTGGACCATGGAAGAGAGTAC CCTATGATGGGTTTCAACCTTGACTCGTAAATACTTCCCTCTGCATGGGGGTGATCGTAGAGAGAATCAA GGCTACCATGTGTTTTCTCATGAGTCAGAAGATGGCTCAGGAAGCCACTGTCACGGTTCCTGGAGGCTGG GCCCTCTCATTGACATGTACACTACTCCTCGCTGCAGGGCACTGTTCCACCTGGATCCAGTTGCAAAGTT TGGAAAAGTTCTCTTAGTTTTACTGGATTCTCAGGGAGCCCTCTGTGGCCTTTTCCTTTATTTTGAGTGC TGTTTCCTTTTTAGGAAAGAACAGCACTGTGGAAATTTGCTTTTCCCCATAGCATGTTAGGAAGGAAGTA CAGGTCGTTACCTAAGCTAGCGTGGGCTTCCAGTTGCCTTAATAGAAGTACTCAAGTCTCTTGGGTAGAG ATCTGGAAGCCTGCAGGAGAGGAGGGGTACTTGTTTTCATTGGAAAACTTTTATGATTAAGGGACTCTTT ACAAACTGGCATCTTAAGTTTTAGTGCCATGTCGCTGTAGTCTGCTGCTTTCCTGGGTGGATGAACCTCT TTATTATGGTAACTTCTGTGCTGCTTTGCCCCTAGCCAAGGCTCTCCTTCCCCAAGAGGTTAGCTTTGGC ATTGGAAGATGAGATTTTTGCTTACCTGTAGTATTTCTGTACATACCTGTTTCCAGCAACTTCAGTGTCA ATGTTTTTGATTTATTTGTGTCTTATAGCTCTAGCTGCTAATAAAGATCCCATGTCCTGCTTTCAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_028018 |
| Insert Size | 1089 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC017605, AAH17605 |
| RefSeq Size | 2354 bp |
| RefSeq ORF | 1089 bp |
| Locus ID | 71955 |
| UniProt ID | Q9CX00 |
| Gene Summary | ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205554 | Ist1 (tGFP-tagged) - Mouse RIKEN cDNA 2400003C14 gene (2400003C14Rik) |
CNY 3,710.00 |
|
| MR205554 | Ist1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2400003C14 gene (2400003C14Rik) |
CNY 3,656.00 |
|
| MR205554L3 | Lenti ORF clone of Ist1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2400003C14 gene (2400003C14Rik) |
CNY 5,230.00 |
|
| MR205554L4 | Lenti ORF clone of Ist1 (mGFP-tagged) - Mouse RIKEN cDNA 2400003C14 gene (2400003C14Rik) |
CNY 5,230.00 |
