Ist1 (NM_028018) Mouse Tagged ORF Clone
CAT#: MG205554
- TrueORF®
Ist1 (tGFP-tagged) - Mouse RIKEN cDNA 2400003C14 gene (2400003C14Rik)
ORF Plasmid: DDK
"NM_028018" in other vectors (4)
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CNY 3,710.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2400003C14Rik; AW536298; mKIAA0174 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG205554 representing NM_028018
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGCTCTGGGTTCAAAGCTGAGCGCTTACGAGTCAATTTGAGATTAGTTATAAATCGTCTCAAAC TGTTGGAGAAAAAAAAAACGGAACTGGCTCAGAAGGCAAGGAAGGAGATTGCTGACTACCTGGCCGCCGG AAAGGATGAGCGAGCACGCATCCGTGTGGAGCACATAATCCGGGAGGACTACCTCGTGGAGGCCATGGAG ATCCTGGAGCTGTATTGTGACCTGCTGCTGGCGCGCTTTGGCCTTATCCAGTCTATGAAGGAGCTAGATT CTGGTCTAGCTGAATCCGTGTCTACACTGATCTGGGCTGCGCCTCGGCTTCAGTCAGAAGTGGCTGAGTT GAAAATAGTTGCCGATCAGCTCTGTGCCAAATACAGCAAGGAATATGGCAAGTTGTGCAGGACCAACCAG ATCGGGACTGTGAATGATAGGCTAATGCACAAACTGAGCGTAGAAGCCCCACCCAAAATCCTAGTGGAGA GATACCTGATTGAAATTGCCAAGAATTACAATGTACCCTACGAACCTGACTCTGTGGTCATGGCAGAAGC TCCTGTTGGGGTGGAGACAGATCTTATTGATGTTGGATTTACGGACGATGTGAAGAAAGGTGGTCCTGGA AGAGGAGGAGGGGGCGGGTTCACAGCCCCCGTTGGGGGACCTGATGGGATTGTGCCCATGCCCATGCCTA TGCCCATGCCATCTCCAAATGCTCCCTTCGCATACCCACTGCCAAAGGGACCATCGGATTTCAGTGGATT GCCAGTGGGGACTTACCAGGCCTTTCCTAACATTCACCCACCTCAGATACCAGCAACTCCCCCATCCTAT GAATCTGTTGATGACATTAATGGTGATAAAACTGTTTCTTCTGCACAGATTGTTGGACCCAAGCCAGAAG CTCCTGCAAAGCCCCCTTCCAGACCTGTGGATAATTACAACACCTTTGTACTGCCAGAGTTGCCATCGGT GCCAGACACACTTCCAACTGCATCTGCTGGAGCCAGCACCTCAGCATCAGAAGACATAGACTTTGATGAT CTTTCCCGGAGATTTGAGGAACTGAAAAAGAAAACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205554 representing NM_028018
Red=Cloning site Green=Tags(s) MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAME ILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQ IGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPVGVETDLIDVGFTDDVKKGGPG RGGGGGFTAPVGGPDGIVPMPMPMPMPSPNAPFAYPLPKGPSDFSGLPVGTYQAFPNIHPPQIPATPPSY ESVDDINGDKTVSSAQIVGPKPEAPAKPPSRPVDNYNTFVLPELPSVPDTLPTASAGASTSASEDIDFDD LSRRFEELKKKT TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_028018 |
ORF Size | 1086 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_028018.2 |
RefSeq Size | 2304 bp |
RefSeq ORF | 1089 bp |
Locus ID | 71955 |
UniProt ID | Q9CX00 |
Gene Summary | ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201264 | Ist1 (untagged) - Mouse RIKEN cDNA 2400003C14 gene (2400003C14Rik), (10ug) |
CNY 3,656.00 |
|
MR205554 | Ist1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2400003C14 gene (2400003C14Rik) |
CNY 3,656.00 |
|
MR205554L3 | Lenti ORF clone of Ist1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2400003C14 gene (2400003C14Rik) |
CNY 5,230.00 |
|
MR205554L4 | Lenti ORF clone of Ist1 (mGFP-tagged) - Mouse RIKEN cDNA 2400003C14 gene (2400003C14Rik) |
CNY 5,230.00 |