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Nae1 (NM_144931) Mouse Untagged Clone
CAT＃: MC201567
Nae1 (untagged) - Mouse NEDD8 activating enzyme E1 subunit 1 (Nae1), (10ug)
CNY 3,984.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 59kDa; Appbp1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC019163 sequence for NM_144931
CCACGCGTCCGGCCTCCTTAGGCGGCTTTGCTGTTCGCGGGCCAGGCGCGGCCATGGCGCAGCCAGGGAA GATACTCAAGGAGCAAAAGTACGACCGGCAGCTGAGGCTGTGGGGTGATCATGGACAAGAAGCTTTGGAA TCTGCTCATGTTTGCTTAATAAATGCAACGGCTACAGGAACTGAAATTCTTAAAAACTTGGTGCTGCCAG GTATTGGATCATTTACAATTATTGATGGAAATCTGGTCAGCGGAGAAGATGCTGGAAACAACTTTTTCCT TCAAAAAAGCAGTATTGGCAAGAACCGAGCTCAAGCTGCCATGGAATTCTTACAAGAACTAAATAGCGAT GTCTCTGGAAGTTTTGTGGAAGAGAGTCCAGAAAACCTTCTAGACAATGATCCTTCATTTTTCTGTAGGT TTACTATTGTGGTTGCAACTCAGCTTCTTGAAAGTACATTGCTGCGTTTAGCAGATGTTCTCTGGAATTC CCAGATCCCTCTTTTGATCTGTAGGACATATGGACTAGTTGGTTATATGAGGATTATTATAAAAGAACAC CCAGTAATAGAATCTCATCCAGATAATGCATTGGAGGATCTACGACTAGATAAGCCATTTCCTGAACTGA GAGAACATCTTCAGTCTTATGACTTGGACCATATGGAAAAAAAGGACCATAGCCATACTCCATGGATTGT GATCATAGCTAAATATTTAGCACAGTGGTATAATGAAACAAATGGACGGATTCCTAAAAGTTATAAAGAA AAAGAGGACTTCAGAGATTTGATAAGACAAGGGATTTTAAAGAATGAAAATGGAGCTCCAGAAGACGAAG AGAATTTTGAAGAAGCCATCAAAAATGTGAACACAGCACTAAATACAACTCAGATCCCAAGCAGTATTGA AGATATATTTAATGATGATCGATGCATAAATATCACCAAACAGACACCAACTTTTTGGATTCTAGCTCGT GCCTTAAAGGAATTTGTGGCCAAGGAGGGTCAAGGAAATTTACCTGTCCGAGGCACAATTCCTGATATGA TTGCAGATTCAAACAAATACATAAAACTGCAGAATGTTTACCGAGAGAAAGCAAAGAAAGATGCTGCTGC TGTAGGCAATCACGTTGCCAAATTGCTTCAGTCTGTTGGCCAGGCACCAGAGTCCATTTCAGAGAAAGAA TTAAAATTGCTTTGCAGCAATTCTGCATTTCTTCGAGTGGTAAGATGTCGATCTTTAGCTGAAGAGTATG GCTTGGATACAGTCAACAAAGATGAAATTATTTCTAGCATGGACAATCCGGATAATGAGATAGTCCTGTA CCTGATGTTGCGGGCTGTTGATCGATTTCATAAACAGCATGGCAGATACCCAGGAGTATCTAACTATCAA GTTGAAGAAGATATAGGGAAGTTGAAGTCCTGTCTTACTGGCTTCCTTCAGGAATATGGGCTGTCTGTAA TGGTGAAAGACGATTATGTCCATGAATTTTGCCGGTACGGAGCTGCTGAGCCACACACCATTGCTGCGTT CCTGGGAGGAGCTGCTGCTCAAGAAGTTATCAAAATAATCACCAAACAATTCGTAATTTTTAATAATACT TATATTTATAGTGGTATGTCACAAACTTCTGCAACTTTCCAGTTGTAGAATAAGCACCATGTGTAATATG TTAATGGTTGGAACTGTAATTGTCTTCATATTGTGCTTTGGTTTGTAAAAATCTGTTAAAGGACGGAAGT TAAATTGTTTCCTTAATAAAAGTTTTTCTCATTTGTAAAAAAAAAAAAAAAAAAG |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_144931 |
| Insert Size | 1605 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC019163, AAH19163 |
| RefSeq Size | 1805 bp |
| RefSeq ORF | 1605 bp |
| Locus ID | 234664 |
| UniProt ID | Q8VBW6 |
| Gene Summary | Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208551 | Nae1 (tGFP-tagged) - Mouse amyloid beta precursor protein binding protein 1 (Appbp1) |
CNY 5,230.00 |
|
| MR208551 | Nae1 (Myc-DDK-tagged) - Mouse NEDD8 activating enzyme E1 subunit 1 (Nae1) |
CNY 3,984.00 |
|
| MR208551L3 | Lenti ORF clone of Nae1 (Myc-DDK-tagged) - Mouse NEDD8 activating enzyme E1 subunit 1 (Nae1) |
CNY 6,650.00 |
|
| MR208551L4 | Lenti ORF clone of Nae1 (mGFP-tagged) - Mouse NEDD8 activating enzyme E1 subunit 1 (Nae1) |
CNY 6,650.00 |
|
| MR208557 | Nae1 (Myc-DDK-tagged) - Mouse NEDD8 activating enzyme E1 subunit 1 (Nae1) |
CNY 3,984.00 |
