United States
Germany
Japan
United Kingdom
China
Mmachc (NM_025962) Mouse Untagged Clone
CAT＃: MC202568
Mmachc (untagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc), (10ug)
CNY 2,400.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1810037K07Rik; CblC |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC054756 sequence for NM_025962
TGCGTTATGGAGCCGCGAGTCGCAGAGCTGAAGCAGAAGATTGAGGACACCTTGTGTCCTTTTGGCTTCG AAGTTTATCCCTTCCAGGTGGCGTGGTACAATGAACTCCTGCCTCCAGCCTTCCACTTGCCCTTCCCAGG ACCTACCCTGGCCTTCCTGGTACTCAGCACACCTGCTATGTTTGACAGAGCCCTCAAACCCTTCTTAAAG AGCTGCCACTTCCAAACACTGAGAGACCCGGTGGATCAATGTGTGTCCTACCACCTGAGGAGTGTTACAG AGAAGTTTCCAGAAGTGCATATGGAAGTCATTGCTGACTATGAGGTACACCCCAATCGGCGACCTAAGAT TCTCGCCCAGACAGCAGCCCATGTGGCAGGTGCTGCTTATTACTACCAACGACAAGATGTGGATGCAGAC CCATGGGGGACCCAGCACATAGCAGGTGTGTGCATACACCCCCGATTTGGGGGCTGGTTTGCCATCCGAG GGGTTATGTTGCTGCCAGGGATTGAAGTGCCAAATTTGCCACCCAGAAAGCCCCCTGACTGTGTGCCTAC AAGAGCTGGCCGCATCACTCTGCTTGAAGGTTTCAATTTCCATTGGCGGGACTGGACTTACCGTGATGCT GTGACTCCTGAAGAACGGTACTCCGAAGAACAGAAGATCTACTTTTCCACCCCACCTGCCCAACGCTTGG CCCTATTAGGCTTAGCCCAACCCTCAGAACACCCTAGCACTACATCTGAGCTTCCTCTTTCCTTGCTTAC TAAACCTCAGAATTCCAGGAGAGCTCGAAGCTGGCTGAGTCCAAGTGTCTCACCACCTGTATCCCCAGGC CCTTGATAACTTTTCCCCTTCAGGATCCCACTCATAGTAGATTTATTTTAAGTAGGATTTGGGTTTTGAA AAAAACACTTTCTCCTTTTTTAGTTTTTTTGAGACAAGGTTCCTCTATGTAGCTCAGACCAGGTTGGCCT CAAGCTAAGAGAGATCCATCTGCCTCTGTCTTCTCCAGAGTGCTGATTTTTTGCACTGTGGAAAAACCAT TTTATTTTAGGTAGGATTTCCTGATAATAGTGCTTCAGACAGTCAAGACTCAATTTACATTGGTCAAGAT AAATCATATGCCAAGGCAGACTCAAAAATCAGCAGTTGACTTCAAATATTAGACTCATGCCCATTACTAT TTTTTTGTTTTTGTTTTTTGTTTTTTCAAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCA CTCTGTTGACCAGGCTGGCCTCAAACTCAGAAATCCACCTGCCTCTGCCTCCCGAGTGCTGGGATTAAAG GCGTGCACTACCAAGCCCGGCTTTGTGTAGTACTCTTAAGATACTATTTAAGAACCATGGTTCTCACTGT TCGTTCCTAAAGCTGCTATCATTTAATGCAGTTCCTCATGTTGTAGTGACCGCAACCATAAAATTATTTT TGTTGCTACCTAATAATTTTGCTAGTTATGAATCAATTGTCTTAGGGAATCCAGTGAAAGGGTTGTTCAA CAACCCCTGGGGGCTGAGAATCACAGATTTAGCAGCTCCTAGGGGTACCCACCACATTGAGCTATGCTGA ATTAATATTTAAGTAAACTACATTGAAACTGCCCCGACCTACAGTGTGCTATTACTAAAGGCTCAGGCAA TCTTATACTTATGCTCAAATAGATGTACTTTGAATTCTGGAATGTTGGAAGGACATGATGTCATAGGATT ATTTTGTCAACCCTAACCAAGAATCCAGAAGAAACTTCCTGACTTTACAGGATCAGAAAGGAATATTCTC TCATCACAGCTAAAATACAAGTAGTTGAATACAATCATATCCAAAGCCAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_025962 |
| Insert Size | 669 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC054756, AAH54756 |
| RefSeq Size | 1889 bp |
| RefSeq ORF | 669 bp |
| Locus ID | 67096 |
| UniProt ID | Q9CZD0 |
| Gene Summary | Catalyzes the reductive dealkylation of cyanocobalamin to cob(II)alamin, using FAD or FMN as cofactor and NADPH as cosubstrate. Can also catalyze the glutathione-dependent reductive demethylation of methylcobalamin, and, with much lower efficiency, the glutathione-dependent reductive demethylation of adenosylcobalamin. Under anaerobic conditions cob(I)alamin is the first product; it is highly reactive and is converted to aquocob(II)alamin in the presence of oxygen. Binds cyanocobalamin, adenosylcobalamin, methylcobalamin and other, related vitamin B12 derivatives.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Mouse models to study the pathophysiology of combined methylmalonic acidemia and homocystinuria, cblC type
,Chern, T;Achilleos, A;Tong, X;Hsu, CW;Wong, L;Poché, RA;,
Dev. Biol.
,PubMed ID 32941884
[MMACHC]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG202551 | Mmachc (tGFP-tagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc) |
CNY 2,850.00 |
|
| MR202551 | Mmachc (Myc-DDK-tagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc) |
CNY 2,400.00 |
|
| MR202551L3 | Lenti ORF clone of Mmachc (Myc-DDK-tagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc) |
CNY 4,750.00 |
|
| MR202551L4 | Lenti ORF clone of Mmachc (mGFP-tagged) - Mouse methylmalonic aciduria cblC type, with homocystinuria (Mmachc) |
CNY 4,750.00 |
