Bnip3 (NM_009760) Mouse Untagged Clone
CAT#: MC203253
Bnip3 (untagged) - Mouse BCL2/adenovirus E1B interacting protein 3 (Bnip3), nuclear gene encoding mitochondrial protein, (10ug)
CNY 2,400.00
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | Nip3 |
Vector | PCMV6-Kan/Neo |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC046603
CGCCTGGCCTCAGAACTGAGCGGCGCAGGCTGCCCCTGCTACCTCTCGGTGACCGCCTCCCAGGCCTGTC GCAGTTGGGTTCGGGCTCCTTTGCCGAGCCACCATGTCGCAGAGCGGGGAGGAGAACCTGCAGGGCTCCT GGGTAGAACTGCACTTCAGCAATGGCAATGGGAGCAGCGTTCCAGCCTCCGTCTCTATTTATAATGGTGA CATGGAAAAAATACTGTTGGATGCCCAGCATGAATCTGGACGAAGTAGCTCCAAGAGTTCTCACTGTGAC AGCCCACCTCGCTCCCAGACACCACAAGATACCAACAGAGCTGAAATAGACAGCCACAGCTTTGGCGAGA AAAACAGCACTCTGTCTGAGGAAGATTATATTGAGAGAAGAAGAGAAGTTGAAAGTATCCTGAAGAAAAA CTCAGATTGGATATGGGATTGGTCAAGTCGACCAGAAAATATTCCCCCCAAGGAGTTCCTTTTTAAACAC CCGAAGCGCACAGCTACTCTCAGCATGAGAAACACAAGCGTTATGAAGAAAGGGGGAATTTTCTCAGCAG ACTTTCTGAAGGTTTTCCTTCCATCTCTGTTACTGTCTCATCTGCTGGCCATTGGCTTGGGGATCTACAT TGGAAGGCGTCTGACAACTTCCACTAGCACCTTCTGATGAAGATTTGGATCTGACTTCTGTTCACACAGT GAGGATTCATGTCTGAGCTGTGACAGCTAATTGAAGAGCTAGCATGGTCCTTGGGTGTTTGCACTATGTG TGTTAATTTGTTCTGTAAATGCGGTGTTCCTGATTTAGTGAGACAGAATAGACTCTTGTCATGACCTATA ATTATACCTATGGGATCAATTAATAAGCATGTCAGACTGATGCCTATTGTAGCATTTACTAGTAAATTTC TTTGAATATTAGATATAAATAGTAAATTTCTTTGAATATTAGATATAAATAGTATAAATAATTTTAATAT AGTAGCAATGGTGTATTTAAAACTGATCTGTAATCATAATCCAGTTAAGACACTTTACACTTCAAAAGAA CAAGTAAGTGATATTAAAATCATTAAAGGGTTTTCCCCAAAGGAATATTGTGGCCTTATAATCCTATTAC ATAGCAGAAAATTAAAGGGTGCGTGCGGGTTATCTGTAAAGGCCTCTAACTTTGTGAACTGAGTAGCAAG TAGAAGCTAAGGTTACCCACGAACCCCACTTTGCAGTTCCCCCTCGTCCTGCTTTGCAGGATGAGGATTC TCGCCTTGCTGTCCACCATTACCTTGGGTGGTCAGTTTTGTTGCAGGCACCTTTATCACTCTGCTGAATT CTCTTGTTTGTATTTAAATCTTATGTGCTCTGAAAATCAGCTGTCTACTTTAAAAGACTGGAAATGAAAA ACAAAATCTTTGCCAAATCCACTGGAGAATACTATATTTGCATATCCATTAATCAGGATCCAAAGCTGCT TAAATCGGGCGTCCTCCCAGTCTTCTCTTTTCTATAGCATGGTTTTAAAGCCTGCCTCCTTGACATGCTG TATATATCCTGTTGTATTTGTTTCACTGTCCCACACTTAACTCAGGTGTGCTAAAATAAAAGTGACATTT TAACAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
Restriction Sites | RsrII-NotI |
ACCN | NM_009760 |
Insert Size | 564 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC046603, AAH46603 |
RefSeq Size | 1720 bp |
RefSeq ORF | 564 bp |
Locus ID | 12176 |
UniProt ID | O55003 |
Gene Summary | Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2 (By similarity). Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane may play a critical role in the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix (By similarity). The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix (By similarity). Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR201757 | Bnip3 (Myc-DDK-tagged) - Mouse BCL2/adenovirus E1B interacting protein 3 (Bnip3), nuclear gene encoding mitochondrial protein |
CNY 2,400.00 |
|
MR201757L3 | Lenti ORF clone of Bnip3 (Myc-DDK-tagged) - Mouse BCL2/adenovirus E1B interacting protein 3 (Bnip3), nuclear gene encoding mitochondrial protein |
CNY 4,750.00 |
|
MR201757L4 | Lenti ORF clone of Bnip3 (mGFP-tagged) - Mouse BCL2/adenovirus E1B interacting protein 3 (Bnip3), nuclear gene encoding mitochondrial protein |
CNY 4,800.00 |