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Ncl (NM_010880) Mouse Untagged Clone
CAT＃: MC203667
Ncl (untagged) - Mouse nucleolin (Ncl), (10ug)
CNY 7,776.00
货期*
现货
规格
Product images
经常一起买 (2)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | B530004O11Rik; C23; D0Nds28; D1Nds28; Nucl |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC005460
GCTCTTCCGAGCTGCTCGCTCTCCACACGCGCCGCCGCCGTAATCCGCCACCATGGTGAAGCTCGCAAAG GCTGGCAAAACCCACGGTGAGGCCAAGAAAATGGCTCCTCCTCCAAAGGAGGTGGAAGAGGATAGTGAAG ATGAAGAAATGTCAGAAGATGAAGATGACAGCAGTGGAGAAGAGGAGGTTGTCATCCCTCAGAAAAAAGG CAAAAAGGCTACCACAACCCCAGCAAAGAAGGTGGTTGTTTCACAAACAAAAAAGGCTGCAGTTCCCACA CCAGCTAAGAAAGCAGCTGTGACCCCAGGCAAAAAGGCAGTAGCCACACCAGCTAAGAAAAACATTACAC CAGCCAAAGTCATTCCAACACCGGGTAAGAAGGGAGCTGCACAAGCAAAAGCGTTGGTACCAACTCCTGG TAAAAAGGGAGCTGCCACTCCAGCTAAGGGGGCTAAGAACGGTAAGAATGCCAAGAAGGAAGACAGTGAT GAGGATGAAGATGAAGAGGATGAAGAAGATAGCGATGAGGATGAAGATGATGAGGAAGAGGATGAGTTTG AGCCACCAATAGTAAAAGGAGTGAAGCCAGCAAAAGCAGCTCCTGCTGCTCCTGCCTCCGAGGATGAGGA AGATGATGAGGATGAAGATGATGAGGAAGAGGAAGATGAAGAAGAGGAAGATGACTCTGAGGAAGAAGTT ATGGAGATCACAACAGCCAAAGGAAAGAAAACTCCTGCAAAAGTTGTTCCTATGAAAGCCAAGAGTGTGG CTGAGGAGGAGGATGAGGAGGAAGAGGATGAAGATGACGAGGATGAGGATGATGAGGAAGAGGATGACGA AGATGATGATGAGGAAGAAGAGGAGGAAGAACCTGTTAAAGCAGCACCTGGAAAACGGAAGAAGGAGATG ACCAAGCAGAAAGAAGCCCCTGAAGCCAAGAAACAGAAAGTAGAAGGCTCAGAACCAACTACACCTTTCA ATCTGTTCATTGGAAACCTTAATCCAAACAAGTCTGTTAATGAATTAAAATTTGCCATCAGTGAACTTTT TGCTAAAAATGATCTTGCTGTTGTGGATGTCAGAACTGGTACAAATAGGAAATTTGGTTATGTGGACTTT GAGTCTGCTGAAGACCTAGAAAAGGCCTTGGAGCTCACTGGTTTAAAAGTGTTTGGCAATGAAATTAAAC TAGAAAAACCAAAAGGAAGAGATAGTAAGAAAGTTCGAGCTGCAAGAACACTTCTAGCCAAAAACCTCTC TTTCAACATCACTGAGGATGAATTAAAGGAAGTGTTTGAAGACGCCATGGAGATCAGATTAGTCAGCCAG GATGGGAAAAGTAAAGGGATTGCTTATATTGAATTTAAGTCTGAAGCTGATGCAGAGAAAAATTTGGAAG AAAAGCAGGGGGCAGAAATTGATGGACGATCTGTTTCACTCTACTATACTGGAGAGAAAGGTCAAAGGCA AGAGAGAACTGGAAAGACCAGCACTTGGAGTGGTGAATCAAAGACTTTGGTTTTAAGTAACCTTTCCTAC AGTGCAACAAAAGAAACTCTTGAGGAAGTATTTGAGAAAGCAACTTTTATCAAAGTGCCCCAGAACCCAC ATGGCAAACCTAAAGGGTATGCATTTATAGAATTTGCTTCATTTGAAGATGCTAAAGAAGCTTTAAATTC CTGTAATAAAATGGAAATTGAGGGCAGAACAATCAGGCTGGAGTTGCAAGGATCCAATTCGAGAAGTCAA CCATCCAAAACTCTGTTTGTCAAAGGTCTGTCTGAGGATACCACTGAAGAGACCTTAAAAGAATCATTTG AGGGCTCTGTTCGTGCAAGAATAGTCACTGATCGGGAAACTGGTTCTTCCAAAGGGTTTGGTTTTGTAGA CTTTAATAGTGAGGAAGATGCCAAAGCTGCCAAGGAGGCCATGGAAGATGGAGAAATTGACGGAAACAAA GTTACCTTGGACTGGGCCAAACCTAAGGGTGAAGGTGGCTTTGGTGGTCGAGGTGGAGGCAGAGGAGGTT TCGGAGGCAGAGGTGGAGGCAGAGGTGGAAGAGGTGGATTTGGAGGAAGAGGCCGGGGAGGCTTTGGAGG TAGAGGAGGCTTCCGAGGCGGCAGAGGAGGAGGGGGAGACTTCAAGCCACAAGGAAAGAAGACGAAGTTT GAATAGTTCCTTCCATCCCATTCTTTCCCTCTTCATTTTAAAGAAAGGACTCTGGGGTTTTTACTCTGTT ACCTGTTCAATGACAGAGCCTTCTAAGGACATTCCAAGACAGTAAAGATCCTAAACTCTGTTGCCAGACT TAATACTTCCTAGAGGTTACATTTGGATTGTTGCTTAAGAGCTTGGCAAGACCAACTCTGCCCAGTGGGA ATTTCAGTTGTGTTCCTACTGCTTTCAAGAATTCCCAGCTACAAATGTGGAAATTGGTGGGGAACAGCTT CATCATGATTCTGATCTGGAAGATTTAATTTGTATCTTAGAAGGGTGTGTACTGGAAAAGTGGTTGATAG AGCACATGGATCAGTGCTGGAAATACCATACAAGAGCTTGTGTGGTATGTAATTCTATTCCCAGCATAAT AAGAAAAAACAGTACAGTGCAATCTGAAATGACCTGGACAAGGAATGGTACCCCATTGGGTTGAAAAATT CACATAGTCAGGATGGTTGCACATGCCTTTAACCCCAGCACTTGGGAGGGAGAGGCTGGTAGATCTGAGT TTGATCTGATGTACAGAGAGTTCCAGAACAGCCAGGGCTGTTTATTAGAGAAAAAACCCTGTCTTGAAAA AACAAGAAAAAAAAACCTCACAGACAACCAAATACAGAGTTTTAGCCACTGAGTACCAAGTCTCAGCAGC AAGCTGGGAGTGACGTAATCCAATCCCATGGGAGGCTAAAGCAGTCTATGACTTCAATGCCAGTTGGGTC TGCAGAACTGAGTTGCTGGATAGTGAGGGATACATAGAAACTTTGTCTTGAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_010880 |
| Insert Size | 2124 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC005460, AAH05460 |
| RefSeq Size | 3005 bp |
| RefSeq ORF | 2124 bp |
| Locus ID | 17975 |
| UniProt ID | P09405 |
| Gene Summary | Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats (By similarity).[UniProtKB/Swiss-Prot Function] |
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