Mettl1 (NM_010792) Mouse Untagged Clone

CAT#: MC203767

Mettl1 (untagged) - Mouse methyltransferase like 1 (Mettl1), (10ug)



  "NM_010792" in other vectors (4)

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2810012D02Rik
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC012649
CCACGCGTCCGCGGACGCGTGGGCGCGAGCTCGGCTCTCCACGTGGACTGCGTGGCATGATGGCGGGAGC CGAAGCCCCCCAGCCGCAGAAGCGCTACTACCGGCAGCGCGCCCACTCCAACCCCATGGCAGACCACACA CTGCGCTACCCTGTGAAGCCAGAGGAAATGGACTGGTCTGAGCTTTACCCAGAGTTCTTTGCTCCGCTTA ATCAAAATAAGAACCATGATGATCCAAAGGATGAGAAAGAAAAGCACTCTGGGGCCCAAGTGGAGTTTGC AGACATAGGCTGTGGCTATGGTGGCTTGTTAGTGGCACTGTCACCGCTCTTCCCAGATACCCTGATTCTG GGTCTGGAGATTCGGGTGAAGGTGTCGGACTATGTGCAGGACAGGATTCGGGCCCTCCGTGCAGCTCCCG GGGGCGGATTCCAGAACATCGCCTGTCTCCGAAGTAACGCCATGAAACACCTTCCTAATTTCTTCCGCAA GGGCCAGCTGGCAAAGATGTTCTTCCTCTTCCCGGACCCACACTTTAAGCGAACGAAGCATAAATGGAGA ATCATCAGCCCTACGCTTCTGGCAGAGTATGCCTACGTGCTGAGAGTCGGGGGCCTGGTGTACACCGTCA CCGACGTGCCGGAGCTGCATGAGTGGATGTGCACCCACTTTGAAGAACACCCACTATTTGAGTGTGTGCC TCTTGAAGAGCTAAGTGAAGACCCCATTGTGGAACATCTAGGCAGTTCAACTGAGGAAGGAAAGAAAGTT CTACGCAATGGGGGAAAGAATTTCCCAGCCGTCTTCCGAAGAATACAGGATCCCCTCCTCCAGGCAGTGA CCCCCAACCCCACCCTGCCTTAGCCAGACCCCCTAGAAGGTGACTGGAAAAAAGATCAAAAAAAAAAAAA AAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_010792
Insert Size 807 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC012649, AAH12649
RefSeq Size 919 bp
RefSeq ORF 807 bp
Locus ID 17299
UniProt ID Q9Z120
Gene Summary Methyltransferase that mediates the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed:29983320). Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Also acts as a methyltransferase for a subset of internal N(7)-methylguanine in mRNAs (PubMed:29983320). Internal N(7)-methylguanine methylation of mRNAs regulates translation (PubMed:29983320). Also methylates a specific subset of miRNAs, such as let-7. N(7)-methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA) (By similarity). Acts as a regulator of embryonic stem cell self-renewal and differentiation (PubMed:29983320).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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