Fyn (NM_008054) Mouse Untagged Clone

CAT#: MC204024

Fyn (untagged) - Mouse Fyn proto-oncogene (Fyn), transcript variant 2, (10ug)



  "NM_008054" in other vectors (4)

CNY 3,984.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
FYN Rabbit monoclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI448320; AW552119
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC032149
CCACGCGTCCGAAACAAAGCAAAGGAACAGGTGGTGGGGAGTTTGCTGCATGGATGGGTGCCCCAATCGA TCGATCGAGTCAGTCCGACCGCTGGTTTCGAAGACATGTGGTGTATATAAAGTTTGTGATAGTTGGTGGA AATTTGGGAGCTTGGATAATGGGCTGTGTGCAATGTAAGGATAAAGAAGCAGCGAAACTGACAGAGGAGA GGGACGGCAGCCTGAACCAGAGCTCTGGGTACCGCTATGGCACAGACCCCACCCCTCAGCACTACCCCAG CTTCGGCGTGACCTCCATCCCGAACTACAACAACTTCCACGCAGCTGGGGGCCAGGGACTCACCGTCTTT GGGGGTGTGAACTCCTCCTCTCACACTGGGACCCTACGCACGAGAGGAGGGACAGGAGTGACACTGTTTG TGGCGCTTTATGACTATGAAGCACGGACGGAAGATGACCTGAGTTTTCACAAAGGAGAAAAATTTCAAAT ATTGAACAGCTCGGAAGGAGACTGGTGGGAAGCCCGCTCCTTGACAACCGGGGAAACTGGTTACATTCCC AGCAATTACGTGGCTCCAGTTGACTCCATCCAGGCAGAAGAGTGGTACTTTGGAAAACTTGGCCGCAAAG ATGCTGAGAGACAGCTCCTGTCCTTTGGAAACCCAAGAGGTACCTTTCTTATCCGCGAGAGCGAAACCAC CAAAGGTGCCTACTCACTTTCCATCCGTGATTGGGATGATATGAAAGGGGACCACGTCAAACATTATAAA ATCCGCAAGCTTGACAATGGTGGATACTATATCACAACGCGGGCCCAGTTTGAAACACTTCAGCAACTGG TACAGCATTACTCAGAGAAAGCTGATGGTTTGTGTTTTAACTTAACTGTGGTTTCATCAAGTTGTACCCC ACAAACTTCTGGATTGGCTAAAGATGCTTGGGAAGTTGCACGTGACTCGTTGTTTCTGGAGAAGAAGCTG GGGCAGGGGTGTTTCGCTGAAGTGTGGCTTGGTACCTGGAATGGAAATACAAAAGTAGCCATAAAGACCC TTAAGCCAGGCACCATGTCTCCGGAGTCCTTCCTGGAGGAGGCGCAGATCATGAAGAAGCTGAAGCATGA CAAGCTGGTGCAGCTCTACGCGGTCGTGTCTGAGGAGCCCATTTACATCGTCACGGAGTACATGAGCAAA GGAAGTTTGCTTGACTTCTTAAAAGATGGTGAAGGAAGAGCTCTGAAGTTGCCAAACCTTGTGGACATGG CGGCACAGGTTGCTGCAGGAATGGCTTACATCGAGCGCATGAATTATATCCACAGAGATCTGCGATCAGC AAACATTCTAGTGGGGAATGGACTAATTTGCAAGATTGCTGACTTTGGATTGGCTCGGTTGATTGAAGAC AATGAATACACAGCAAGACAAGGTGCGAAGTTTCCCATTAAGTGGACAGCCCCCGAAGCGGCCCTGTATG GAAGGTTCACAATCAAGTCTGACGTATGGTCTTTTGGAATCTTACTCACAGAGCTGGTCACCAAAGGAAG AGTGCCATACCCAGGCATGAACAACCGGGAGGTGCTGGAGCAGGTGGAGAGAGGCTATAGGATGCCCTGC CCACAGGACTGCCCGATCTCCCTGCACGAGCTCATGATCCACTGCTGGAAAAAGGATCCGGAAGAGCGCC CGACCTTCGAGTACTTGCAGGGCTTCCTGGAGGACTACTTTACGGCCACAGAGCCCCAGTATCAGCCCGG TGAAAACCTGTGAGAGCCTGCGCTTCAGACGCCTCTTCCCGAGGCCTCCCTACCCCTCCCCATTAGCTTC CAATTCTGTAGCCAGCTGCCCCAGAGCAGCGAGAACCGTCCAGGATCAGATTGCATGTGACTCTGAAGCT GAACTTCCACGGCCCTCATTAATGACACTTGTCCCCCAGTCCGAACCTCCTCTGTGAACCATCTGAGACA GAAGCGTGTTATTTCTCAGACTTGGAAATGCATTGTATCGATGTTATGTCAAAGGCCAAACCTCTGTTCA GTGTAAATAGCTGCTCCTGTGCCAACAATCCCAGTGCTTTCCTTTTTTAAAAAAGAAAAAGCAAATCCTA TGTGATTTTAACTCTGTCTTCACCTGATTCAACTAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_008054
Insert Size 1605 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC032149, AAH32149
RefSeq Size 2149 bp
RefSeq ORF 1605 bp
Locus ID 14360
UniProt ID P39688
Gene Summary Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) uses an alternate in-frame exon in the coding region compared to variant 1. It encodes an isoform (b) with a different and shorter internal segment compared to isoform a. Variants 2 and 3 encode the same isoform (b).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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