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Itch (NM_008395) Mouse Untagged Clone
CAT＃: MC205232
Itch (untagged) - Mouse itchy, E3 ubiquitin protein ligase (Itch), transcript variant 1, (10ug)
CNY 6,336.00
货期*
现货
规格
| Cited in 1 publication. |
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 6720481N21Rik; 8030492O04Rik; A130065M08; AIP4; C230047C07Rik |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC062934
ACCGGCTGCCATCTTAGCGGAGGGACTGAGGAGTCGTCGCCGCCCCGAGTCCCGGTAACATGCATTTCAC AGTGGCCTTCTGGAAACAACGCCTTAACCCTAAGAAGTGACTCAGCCATTGAGAACTTCAGGTTTGCTGT CTTATTGATGGTATGTCTGACAGTGGACCACAGCTTGATTCCATGGGTAGTCTGACCATGAAATCTCAAC TTCAGATCACTGTCATCTCAGCCAAACTTAAAGAAAATAAAAAGAATTGGTTTGGACCAAGTCCTTATGT AGAAGTCACAGTAGATGGACAGTCAAAGAAGACAGAAAAATGCAATAATACAAACAGTCCCAAGTGGAAG CAGCCCCTCACAGTTATTGTTACCCCTACGAGTAAATTATGTTTTCGTGTGTGGAGTCACCAGACCCTGA AGTCTGATGTTTTATTGGGAACTGCTGGATTAGATATTTATGAAACATTAAAGTCAAACAATATGAAACT TGAAGAAGTAGTTATGACTTTGCAGCTTGTAGGTGACAAAGAGCCAACAGAGACGATGGGAGATTTGTCA GTTTGTCTTGATGGGCTGCAAGTAGAAGCTGAGGTTGTTACTAACGGTGAAACGTCATGCTCCGAGAGTA CTACACAGAATGATGATGGCTGCAGAACCAGAGATGATACAAGAGTGAGCACAAATGGATCAGAGGACCC TGAGGTTGCAGCGTCAGGGGAAAACAAGAGGGCCAATGGGAACAATTCTCCGTCCCTTTCAAATGGTGGT TTTAAGCCTTCTAGACCTCCTAGACCTTCGAGACCACCTCCACCCACCCCACGAAGACCAGCTTCTGTCA ATGGCTCACCATCCACGAATTCTGACAGTGATGGATCTAGTACAGGCTCCTTGCCACCAACAAATACAAA TGTAAATACAAGTACATCTGAAGGAGCAACATCTGGATTAATAATTCCTCTTACTATATCTGGAGGCTCG GGCCCTAGGCCTCTGAATACTGTAAGCCAAGCTCCCCTACCACCTGGGTGGGAGCAGAGAGTAGACCAGC ATGGGCGTGTTTACTATGTAGACCATGTTGAAAAGCGAACAACATGGGATAGACCAGAACCTCTACCTCC TGGCTGGGAACGGCGTGTGGACAATATGGGACGTATTTATTATGTTGATCATTTCACAAGAACCACAACA TGGCAGAGGCCAACCTTGGAATCTGTCCGGAACTATGAACAGTGGCAGCTACAGCGTAGTCAGCTTCAGG GAGCAATGCAGCAGTTTAACCAGAGATTCATCTATGGGAATCAAGATTTGTTTGCTACATCACAAAACAA AGAATTTGATCCGCTTGGTCCGTTGCCCCCTGGATGGGAGAAGAGAACTGATAGCAACGGCAGAGTGTAT TTTGTCAACCACAACACTCGGATTACTCAGTGGGAAGACCCCAGAAGCCAAGGTCAGTTAAATGAAAAGC CCTTACCAGAAGGCTGGGAAATGAGATTCACAGTGGATGGAATTCCATATTTTGTGGACCACAATAGAAG AGCAACTACTTATATAGATCCACGAACGGGAAAATCAGCCTTAGACAATGGGCCCCAGATAGCCTATGTG CGGGACTTCAAGGCAAAAGTTCAGTATTTCCGGTTCTGGTGCCAGCAACTGGCCATGCCACAGCATATAA AGATCACAGTGACAAGAAAAACATTGTTTGAGGATTCCTTTCAGCAGATCATGAGCTTCAGCCCGCAAGA CCTGAGAAGACGTTTGTGGGTGATTTTCCCAGGAGAAGAAGGTTTAGATTATGGAGGTGTAGCAAGAGAA TGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCCAATGTATTGCCTGTTTGAATATGCAGGGAAGGATA ACTACTGCCTGCAGATAAACCCCGCTTCTTACATCAATCCAGACCACCTGAAATACTTTCGTTTTATTGG CAGATTTATTGCCATGGCTCTGTTTCATGGGAAATTCATAGATACCGGATTTTCTTTACCATTCTATAAA CGTATCCTGAATAAACCAGTTGGACTGAAGGATTTAGAATCTATTGATCCAGAATTTTATAATTCGCTCA TCTGGGTTAAAGAAAACAACATTGAAGAATGTGGTTTGGAAATGTACTTCTCAGTTGATAAAGAAATTCT AGGTGAAATTAAGAGTCATGATTTGAAACCCAATGGTGGCAATATTCTTGTGACAGAAGAAAACAAGGAG GAATACATCAGGATGGTAGCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACACAAGCTTTCTTTG AGGGCTTTAATGAAATTCTTCCTCAGCAATATTTGCAATATTTTGATGCGAAGGAATTAGAGGTTCTTCT GTGTGGGATGCAGGAGATTGATTTGAATGACTGGCAGAGACATGCCATCTACCGCCACTACACCAGAACA AGCAAGCAGATCATGTGGTTTTGGCAGTTTGTTAAAGAAATTGATAATGAGAAGAGGATGAGACTTCTGC AGTTTGTTACTGGAACCTGCCGATTGCCAGTGGGAGGATTTGCTGACCTTATGGGGAGTAATGGACCACA GAAGTTCTGCATCGAAAAAGTTGGCAAAGAAAATTGGTTACCCAGAAGCCATACTTGTTTTAACCGCCTG GACCTCCCACCTTACAAGAGCTATGAGCAACTGAAGGAAAAGCTGTTATTTGCCATTGAAGAAACTGAAG GATTTGGACAAGAGTAATTGTTTAGACCTTAAGCCACCAGAGGCCAGGGACTTGTCTGCAGTGTCTGTCC CCTTCCTTCTCTGCCTGTTGCACATCTTATTGTAAACTTGGACTGTGACTAGAGAGTTACCTGAGAGTAA GTAAATTAATGTTATCGTTGAGATCTTCCAGTCATTCTGGATTTCTACTCAACGTTTCCAGAAAGCAGAT CTACAAACTGTAGTCAGAAACTTAACATGAGATTTAACACAGCGAGGAAATTTCCCTCGTCTTATTCCAC TAGATTGTTCCCTTAACAACAGTTTGCTGTATGTCCGAAGTCTCACTTGGGCATAGGTCTCTTAGTGGGT ATTGTAAAGGATTCTGCAGGGACATCTCGGAAACGCAATACACAAGGTCTTCCTGTAAAGCCTCGCTCAG AGGTGCAAGCTTAACTCAGTGTCTGGGCACATGAGCAGCTGCTGCTTGTGCTCTCCGCTCGTCCCAGCCT GGCCTGACTGTTGTTTTTCATTCCTGGAGCATGAATCAGTGCACCATTCTGATGCTATTCCTGGAAGTGA TGCAGGACTTGCATTTTTCTACAAAGTAGTTTTGTCTATTTGAATTCAGGGAAAACTTGGTCCCCGCCAA CATATGACCATACCTCTCTTCATTCAGTTTGTAATTGTTCTATTGCCTTGCAAATACTAAACTTTACAAA CAGTATCAGCACTGTGGTTCCTTCACGTTTTTAGAAATTAACCTAGAATTAAAATAAGTTCAGATGAATT CATCTAAGATCATTCTTGATTTTTTTAAATCCTACACATAAATTGAAGCTTTATGGTGTTGAAGTAATCC AACACCTTAGTTACATTTCATTTTTGCTAAACTATTCAAGTTGCAACCATTTCCTAACACAAATGGAGGT CAGTGGTGCAGACTCTGCAGTCACAACAGCGTCCAGCAGCCACACAGCTGCGCTGTCTTCACACTCCACT CCTGACACAGGAGCAGCCGTGTCTACCCTCTTACATGGTCCTGATCTACAATATTTTTCTCTCCCCTAAA AATTGTAGATTTCCCTTTTCTTTCCATGACTCCTTTATCTTAGGCTGCTCCTTTAGTAACTGATGAATGC ACTTTGACCTTCTCTGTAGGTCATTTACCTTGGCTTAGCATTAGACTGTGAGGATGGTTGCTGCCCAGAC TTTATGGGCAGCAAGATGAAACCTCAGCCAGGTGAGTCCCTATTGTAGCCAAATCCCAAAATTAGCCTTA TTTCTCTAATGGGGACCTTGAAATGTGTTGGTGGCATAGCATAAAGCCAAGAACACCAAGTGTGACAAAC CCCTGTCCCATAGGAAAGAAAGTGTTTCTGTGTCCCTAGCTGCTCATCCTGTTCTCATCCTGGGACAGTT TGAACTCGTGCAGCTGTGCTCCTTAACACTGCCATCCTCAGCAGAGACGTGACAGACGGGTTGCCTCATG AAGAAGTGATCAGTGAAGGGAGAGCTCGGCTCCTCCCCAGCCCTCTTTTTACCCTTATACCACAAGAAAT ACATGTCATCTCTAGAAGCCCTAGTTCTTTCCAAAGTAGGAAATCTCTGCCTTTTTGTTGGTAGGGTTAA AAAAAAATGTATATGCTAATTGCTTTGTCTTACAGAGCGAATGTCTGCCAACTATCCATTCACTACATAG GCCTGAACTTTGGTAATAAATGGCTATAAATATTAAGCCTTCTACAAAGACTTCCTATTGAATCCAATTC TTGTACTAAAACATAGTTATCTACCATATTCACTAGAGATTTATGAGATTAAATTAAGAGATAATGTAAG AAAATAGACTTTGTTGATTCTGCATAATGCTTCATGATTCATTTAGGGACCTAGAAATATTATGTGAAAA TGTATAAATATCATCCAAAAGGCTTTCTGCCCTATATTTTTAAAATACAGAAGTTATATTTGAAGTAGTC CTGGCCCTAGTTCTATAGGGCTTGGCTATTTAATATTTTTATGGAAGAAACGTTTAGTTCTGGAAAAGGT AAATGCTTGTATATATTTTTGCAGCCCTGGAGTCTCCCAACTCCATTTCTTCCTTTAATTTAATTGATCA CATGTATATGTCTTCCCTGCTGTGTTAGAAAAACGGGGGCTGGATATCCCAGGAGTCAGAGCGTATATAA GACAATGCACCTAAACAGCAGGCACATTGACCAGATGCTCTCAAATTTTGGTCCAAAATGAGTATTTGGG AATAGATTTATTGTGAATGTTTAATTAGCGATTTTCCTTAAATCTGGATGGACTAAGTTGCTTTTAAGAC CTTTTTCTCTGTAAGCATCGTAAATGCTAATCAACCTGTTAAGTTTTTTTTTTTCTTACTGCATGTTACA TTGAAATAAAAACAGAGTAAAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_008395 |
| Insert Size | 2595 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC062934, AAH62934 |
| RefSeq Size | 5163 bp |
| RefSeq ORF | 2595 bp |
| Locus ID | 16396 |
| UniProt ID | Q8C863 |
| Gene Summary | Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:15358865, PubMed:16446428, PubMed:17592138, PubMed:18628966, PubMed:20392206, PubMed:25632008). It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation (By similarity). Involved in the control of inflammatory signaling pathways (By similarity). Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways (By similarity). Promotes the association of the complex after TNF stimulation (By similarity). Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains (By similarity). This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1 (By similarity). Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways (By similarity). Regulates the transcriptional activity of several transcription factors involved in immune response (PubMed:15358865, PubMed:11828324). Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages (By similarity). Mediates JUN ubiquitination and degradation (PubMed:15358865). Mediates JUNB ubiquitination and degradation (PubMed:11828324, PubMed:15358865). Critical regulator of type 2 helper T (Th2) cell cytokine production by inducing JUNB ubiquitination and degradation (PubMed:11828324). Involved in the negative regulation of MAVS-dependent cellular antiviral responses (By similarity). Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteasomal degradation (By similarity). Following ligand stimulation, regulates sorting of Wnt receptor FZD4 to the degradative endocytic pathway probably by modulating PI42KA activity (By similarity). Ubiquitinates PI4K2A and negatively regulates its catalytic activity (By similarity). Ubiquitinates chemokine receptor CXCR4 and regulates sorting of CXCR4 to the degradative endocytic pathway following ligand stimulation by ubiquitinating endosomal sorting complex required for transport ESCRT-0 components HGS and STAM (By similarity). Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination (PubMed:18628966). Ubiquitinates SNX9 (By similarity). Ubiquitinates MAP3K7 through 'Lys-48'-linked conjugation (PubMed:25632008). Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP (By similarity). Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID (PubMed:20392206). Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the shorter transcript. Variants 1 and 2 encode the same protein. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Itch WW domains inhibit its E3 ubiquitin ligase activity by blocking E2-E3 transthiolation
,Riling, C;Kamadurai, H;Kumar, S;O'Leary, CE;Wu, KP;Manion, EE;Ying, M;Schulman, BA;Oliver, PM;,
J. Biol. Chem.
,PubMed ID 26245901
[ITCH]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211000 | Itch (tGFP-tagged) - Mouse itchy, E3 ubiquitin protein ligase (Itch) |
CNY 7,890.00 |
|
| MR211000 | Itch (Myc-DDK-tagged) - Mouse itchy, E3 ubiquitin protein ligase (Itch), transcript variant 1 |
CNY 6,336.00 |
|
| MR211000L3 | Lenti ORF clone of Itch (Myc-DDK-tagged) - Mouse itchy, E3 ubiquitin protein ligase (Itch), transcript variant 1 |
CNY 9,030.00 |
|
| MR211000L4 | Lenti ORF clone of Itch (mGFP-tagged) - Mouse itchy, E3 ubiquitin protein ligase (Itch), transcript variant 1 |
CNY 9,030.00 |
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