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Slc1a1 (NM_009199) Mouse Untagged Clone
CAT＃: MC205239
Slc1a1 (untagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1), (10ug)
CNY 3,904.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | D130048G10Rik; EAAC1; EAAC2; EAAT3; MEAAC1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC065099
GCGCTGACAGCGGCCGGTGCGCGTTGTCTCCACTGTGCCCTGCATCCCGCATCTCGCATCGGCCAGGCTA CCCGACTCATCGCAAACGTCAGTGCTCACCATGGGGAAGCCCACGAGCTCGGGATGTGACTGGCGCCGCT TCCTACGGAATCACTGGCTGCTGCTCTCCACCGTGGCCGCCGTGGTACTAGGAATTGTCTTAGGAGTCGT GGTTCGAGGACACAGTGAGCTCTCAAATCTGGATAAATTCTACTTTGCTTTTCCTGGGGAAATTCTGATG AGGATGCTGAAGCTGGTCATTTTGCCGCTGATCGTATCCAGCATGATCACAGGTGTCGCTGCACTGGATT CCAATGTGTCTGGGAAGATTGGTCTGCGCGCTGTAGTATATTATTTCTCCACCACCGTCATTGCTGTAAT CCTAGGTATTGTGTTAGTTGTGAGTATCAAGCCTGGTGTCACTCAGAAAGTGAATGACATCAACAGGACG GGTAAAACCCCTGAAGTCAGCACCATGGATGCCATGTTGGACCTGATCAGGAACATGTTCCCTGAGAATC TGGTCCAAGCCTGTTTTCAGCAGTACAAAACCAAGCGGGAAGAGGTGAAGCCTGTGGGCGATCCTGGGGG GAACGCAACGGAGGTGTCTGTCACCACAGCCATGACAACAATGTCTGAGAACAAGACAAAGGAATACAAG ATCGTGGGCCTGTACTCAGACGGCATCAATGTCCTGGGCTTGATTATCTTCTGCCTCGTCTTTGGCCTTG TCATTGGGAAAATGGGAGAAAAGGGGCAGATTCTGGTGGACTTCTTCAATGCCTTGAGTGACGCCACCAT GAAAATCGTCCAGATCATCATGTGCTACATGCCGATTGGCATTTTGTTCCTAATTGCTGGGAAGATCATA GAAGTTGAAGACTGGGAAATATTCCGCAAGCTGGGCCTTTACATGGCCACTGTCCTGAGCGGGCTTGCAA TCCACTCCCTCATAGTTCTGCCCCTGCTCTATTTCATAGTTGTGCGGAAGAACCCTTTCCGCTTTGCCTT GGGTATGGCGCAGGCTCTCCTGACAGCTCTCATGATCTCGTCCAGTTCGGCAACCCTGCCAGTTACATTC CGCTGTGCGGAAGAAAAGAACCAGGTAGACAAGAGGATCACGAGATTTGTGCTGCCTGTTGGTGCCACCA TCAACATGGACGGCACTGCGCTCTACGAAGCTGTGGCAGCCGTGTTTATTGCGCAACTGAATGGCTTGGA CCTAAGCATTGGGCAGATCGTCACCATCAGCATTACAGCCACCGCTGCCAGCATTGGAGCTGCTGGGGTG CCCCAGGCTGGCCTGGTGACCATGGTGATCGTGCTGAGTGCTGTGGGGCTGCCTGCCGAGGACGTCACCC TGATCATTGCTGTTGACTGGCTCCTGGACCGGTTCAGGACCATGGTGAACGTCCTGGGTGATGCGTTTGG GACGGGCATCGTAGAGAAGCTCTCGAAGAAGGAGCTGGAGCAGATGGATGTTTCGTCTGAAGTCAACATC GTGAACCCCTTTGCCCTGGAACCCACAACCCTCGATAACGAAGACTCAGATACCAAGAAGTCTTATGTCA ATGGGGGCTTCGCGGTAGACAAATCTGACACCATCTCGTTCACTCAGACCTCACAGTTCTAGATGCCTGA CCTCAGATTGAGGCCTGGGATTGTGAAGGGCGTCTCCACAGGAGCCATCTCCTAGCAAACTCCGACATTA AGGAACGAGAAGGACACTAAGAGTCAACTGTACATTTAGTTTGATAAACAGACCTCCAGATTATTTTCTA TATTTGACTTTATAGCCTTGGTTCTCTGGGTTTAGGGATTTGGGGTGAGATGAACTGAAAGGAAATTAAG AAAGTTGTGTTATCTGGATTTTCTAATTCTATACAACAGAGTTTGGAAGTATATGAAGTAGTAACTGTTA GGATTAGGTCATAGATATGGAAGAGAAATTGGTTTCTCATGCATAGACCAGTGTTTGGGGTTTTTAAACA ATATTATTGGCTACAAATTTTTACTCAGGCTTTCTATTGGCAGGACTTCCTTTGCCTTTTTACTTTTATA GATTATAATGCATCTCAAAAGCCCTACCCAGTTAATGTGCCAAATTTTCCATTTTGACCTCATCTCCAGC CACTCTCAAACTACCCTGGGGCGGGGGGAGCAAAAAAGATCAGCATAGTTCTGCAATAACAGTTTAAAGA TAGTTGTGGGGTTTAGGGGAAGGGAAAGGGTTTTTTTATTCAATGTACTGTATTGAGACACTGGTAGCTG ACAGCCAGTGTTCGGTATAGAACTATATGTATATGTGTGTATATTTATTATTTTCATGTAATTTGCAAGA CAGAGATCAGTAATGAACTATCAATGTGAAATACGCAGCTTCCCTTGTACTTGAATCAAAACGATAGCTC CAGCCTAGGTGTGAGCTCACCAGAACACTGTCAGGCACTCTGGGATGAGAAATCAAGTTGCTGGCTTACT GTGATTCAAGCCCTAAAGCAGAAACATATTATGGTGAAACTCTAAGATGACACAGCCATTCACGTACAAC ATCTAGGGTCAGGCTCCCGGGAGGGGGAGGCTCCCTGCGAGCATGGAATAAGTACATTTACAAAGGCACT GTAGAGGCAGGAAGTGCTCCCATAGCAACAAAAGGCTTCGATCTTCAAGTAGACTTCAAGACCCACTTCA CAAGGCTGTCACTTTTCTGTTCTTGGTTTTCTCTGCCTGCGCCCCCCACCCCCAGGGCCAAACCAGCAGT GACAAGCCACTGCTGTTTCAAAACGGGGTGGCCTAAATTGAATAAGCCTCATTGCAAGGTGACCAAGCTA TCCTTATACTGTCGTCTTTTTATTTTATCTTCTGGTTTTTTTATTTTTTAGTTTTTGAGACAGCGCCTCT CTACATAGCCCTGGATATCCTGGAACTCACTATGTAGACTAGACTGGCCTCAAACTCACAGAGATCCTCC TGCCTCTGCCTCTTTAGTGCTGGCCTGAAAGGTAGGTACCACCATGCCCAGCTCTACACTGTATTTTTAC AGAAGAAAAGCCAGGCCATAAGCGACTGGTACCAGCGGTTCAGGGACAATACTTCAGTCCTTCCCTGGAG AGGATTGTTCTGGGAATCTCAGCCTTGTGGCTTAGAATCCTCTGCCTGTCTTTCTCCTGCTAATTCCCGA AGATGGCTTATAAAAGTCTACACTTCTGTCCTCATCCTGTAAATAAAACTCAACAAAAACTTGTTCTTAA CTTGGAGACAGGTTCATAACAGCCGTGTTTCTGTAGTGCCCTTAAGTCATCTTAAACCCGTGCTTTTATA TTTAAGAAGCCAGAAATCGTGCCAAAGATAGCAGGAAGGTAACCGAATGCTCAGAGTTGGCCACGCCCAC CTGAAAGCTACCGACTGACCGTCACGGTGACCCTTGACTCCGAACTTTGAAGTACAAATATCTGTATTCT TTATAGGAAGTAAATCTAAATCTAAATGAGGTTGAATGGATATTTTATTTAGGAGTGGATGGTTCTGTCC CCTTATCAGGTGGTTCTCCTTAGTGGCAGTGAATTGGCAGAGCCGTTCACAAGATCATTGGGGTCATCTT GTAACCAGCCACTTCACACACTGTGCTGTTAACTCAAGATGATATGTTCCACTTCCTTCTCAATAAACAT CTCCCCCACTCTTTCTCCCCTTAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_009199 |
| Insert Size | 1572 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC065099, AAH65099 |
| RefSeq Size | 3754 bp |
| RefSeq ORF | 1572 bp |
| Locus ID | 20510 |
| UniProt ID | P51906 |
| Gene Summary | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:12119102, PubMed:18684713). Can also transport L-cysteine (PubMed:30840898). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Plays an important role in L-glutamate and L-aspartate reabsorption in renal tubuli (PubMed:9233792). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (PubMed:9233792). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (PubMed:30840898). Negatively regulated by ARL6IP5 (PubMed:12119102).[UniProtKB/Swiss-Prot Function] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208367 | Slc1a1 (tGFP-tagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1) |
CNY 5,496.00 |
|
| MR208367 | Slc1a1 (Myc-DDK-tagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1) |
CNY 3,896.00 |
|
| MR208367L3 | Lenti ORF clone of Slc1a1 (Myc-DDK-tagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1) |
CNY 6,460.00 |
|
| MR208367L4 | Lenti ORF clone of Slc1a1 (mGFP-tagged) - Mouse solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1) |
CNY 6,460.00 |
