Ext1 (NM_010162) Mouse Untagged Clone

CAT#: MC205589

Ext1 (untagged) - Mouse exostoses (multiple) 1 (Ext1), (10ug)



  "NM_010162" in other vectors (4)

CNY 8,208.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AA409028
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC004741
CCCACGCGTCCGCCACGCGTCCGGATCAGTCCACGGCTTGGGGAAAGGCATCCAGAGAAGGTGGGAGCGG AGAGTTTGAAGTCTTTACAGGCGGGAAGATGGCGGACTGGAGCTGAAAGTGTTGATTGGGAAACTTGGGT GATTCTTGTGTTTATTTACAATCCTCTTGACCCAGGCAGGACACATGCAGGCCAAAAAACGCTATTTCAT CCTGCTCTCAGCTGGCTCTTGTCTCGCCCTTTTGTTTTATTTTGGAGGCGTGCAGTTTAGGGCATCGAGG AGCCACAGCCGGAGAGAAGAGCACAGTGGTCGGAATGGCTTGCACCAGCCCAGTCCGGATCATTTCTGGC CCCGCTTCCCGGACGCTCTGCGCCCTTTCTTTCCTTGGGATCAATTGGAAAACGAGGATTCCAGCGTGCA CATTTCCCCCCGGCAGAAGCGAGACGCCAACTCGAGCATCTACAAAGGCAAGAAGTGCCGCATGGAGTCC TGCTTCGATTTCACCCTTTGCAAGAAAAACGGCTTCAAAGTCTACGTGTACCCGCAGCAGAAAGGGGAGA AAATCGCCGAAAGTTACCAAAACATTCTAGCGGCCATCGAGGGCTCCAGGTTCTACACCTCGGACCCCAG CCAGGCGTGCCTCTTTGTCTTGAGTCTGGATACTTTAGACAGAGACCAGTTATCACCTCAGTATGTGCAC AATTTGAGATCCAAAGTGCAGAGTCTCCACTTGTGGAACAATGGTAGGAATCATTTAATTTTTAATTTAT ATTCTGGCACTTGGCCTGACTACACTGAGGACGTGGGGTTTGACATCGGCCAGGCGATGCTGGCCAAAGC CAGCATCAGTACTGAAAACTTCCGACCAAACTTTGATGTTTCTATTCCCCTCTTTTCTAAGGATCATCCC AGGACAGGAGGGGAGAGGGGGTTTTTGAAATTTAACACCATCCCTCCTCTCAGGAAGTACATGCTGGTAT TCAAGGGGAAGCGGTACCTGACAGGGATAGGGTCAGACACCAGGAATGCCTTATATCACGTCCATAACGG GGAGGACGTCTTGCTCCTCACCACCTGCAAGCATGGCAAAGACTGGCAAAAGCACAAGGATTCTCGCTGT GACAGAGACAACACCGAGTATGAGAAATATGATTATCGGGAAATGCTGCACAATGCCACTTTCTGTCTGG TTCCTCGTGGTCGCAGGCTTGGGTCCTTCAGATTCCTGGAGGCTTTGCAGGCTGCCTGTGTCCCTGTAAT GCTCAGCAACGGATGGGAGTTGCCATTCTCCGAAGTGATTAATTGGAACCAAGCTGCCGTCATAGGCGAT GAGAGATTGCTATTACAGATTCCTTCTACAATCAGGTCTATTCATCAGGATAAAATCCTAGCACTTAGAC AGCAGACACAGTTCTTGTGGGAGGCTTATTTTTCTTCAGTTGAGAAGATTGTATTAACTACACTAGAGAT TATTCAGGACAGAATATTCAAGCACATATCACGTAACAGTTTAATATGGAACAAACATCCTGGAGGATTG TTCGTCCTACCGCAGTATTCATCTTACCTGGGAGATTTCCCTTACTACTATGCTAATTTAGGTTTAAAGC CCCCCTCCAAATTCACTGCAGTCATCCATGCTGTGACTCCCCTGGTCTCTCAGTCCCAGCCAGTGTTGAA GCTTCTTGTGGCTGCAGCCAAATCCCAGTACTGTGCGCAGATCATAGTTCTGTGGAATTGTGACAAGCCT CTACCAGCCAAACATCGCTGGCCTGCCACTGCCGTGCCTGTCATCGTCATTGAAGGAGAAAGCAAGGTTA TGAGCAGCCGGTTTCTGCCCTATGACAACATCATCACTGATGCTGTGCTCAGCCTGGATGAGGACACTGT GCTTTCAACTACGGAAGTGGATTTTGCCTTCACCGTATGGCAGAGCTTCCCAGAGAGGATTGTGGGATAT CCTGCTCGCAGTCATTTCTGGGATAACTCTAAGGAGCGGTGGGGATATACATCCAAGTGGACGAATGACT ACTCCATGGTGTTGACAGGAGCTGCTATCTACCACAAATATTATCACTACCTGTATTCCCATTACCTGCC AGCCAGCCTGAAGAACATGGTAGACCAACTGGCCAACTGTGAGGACATTCTCATGAATTTCCTGGTGTCT GCTGTGACAAAATTGCCTCCAATCAAAGTGACCCAGAAGAAACAGTATAAGGAGACAATGATGGGACAGA CTTCCCGAGCATCCCGCTGGGCCGACCCTGACCACTTTGCCCAGCGACAGAGCTGCATGAATACATTTGC CAGCTGGTTTGGCTACATGCCGCTGATCCATTCTCAGATGAGGCTGGACCCGGTCCTCTTTAAAGACCAA GTCTCAATTCTGAGGAAGAAATACAGAGACATTGAACGACTTTGAGGAAGCCCACCGAGTGGGGGAGGGG AAGCAAGATGGGCGTCCAGCTGCTCTCTCCTCCTTCCCAATGCAGATCCGCTCACGCCCAGCAGTGGAGC CAGACTGTGCCAAGTATCAAAAAATCAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_010162
Insert Size 2241 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC004741, AAH04741
RefSeq Size 2562 bp
RefSeq ORF 2241 bp
Locus ID 14042
UniProt ID P97464
Gene Summary Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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