Vangl2 (BC052195) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Lpp1, Vang1l2, stbm, strabismus
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC052195 CCGATTGCTTGGTTCTGGGTCCCGCCATGGGAGCCTGAGCGCCCTGCAGTCCCCTCCGGCCCCCTGCCCC CGGGGCCTCGAGGGGGAAACAGGCGAGTGGTCTGGGACGGAGCCGGGGTGAGCGACCTCAGGAGCCCCCC TCGTCAACCCCCATCGCCCGCGCTGCCGGTTCTGGAGCGCGGAGTTCGGAAGGACCGGGTGCGCTGCGGA TACAAAGGCGCGGAGCGGAGTGGGGCGTGCGCCCAGTCCACCCGGCTGTTCGCAGTGGCGGGGAGCTGTC GCTTGAATTTCTCTGAGATAAGCCCACTTGTCCAGCAAAATAGAGTCCCTCAGGGTGACGGTTGACTTCC TAAAGGTGCCTCTTGGCCTGAAGAAGCCTGTGCTGAAGGAGGTGGCTGTGGGACCCCCCAAGAGGTCCCA GCCCGCGGCCCTGGAGCGCTACAAGGCACGGCGTTCGGACGCCATGGACACCGAGTCCCAGTACTCGGGC TATTCCTACAAGTCGGGCCACTCCCGCAGCTCCCGGAAGCACAGGGACCGCCGGGACCGACACCGCTCTA AGAGCCGGGATGGGAGTCGTGGAGATAAATCAGTGACGATCCAGGCTCCGGGAGAACCCCTGCTGGACAA TGAGTCCACGAGGGGGGATGAGCGGGATGACAACTGGGGAGAAACAACAACGGTGGTCACGGGCACTTCT GAGCACAGTATCTCCCATGATGACCTCACGCGCATCGCCAAGGACATGGAGGACAGTGTCCCGTTGGATT GTTCCCGCCACCTGGGCGTGGCGGCAGGGGCCATTCTGGCGCTGCTCTCGTTCCTCACCCCGCTGGCTTT CCTGCTGCTGCCTCCACTGCTGTGGCGGGAGGAGCTGGAGCCGTGTGGGACGGCCTGTGAGGGCCTCTTC ATCTCCGTGGCCTTCAAGCTGCTCATCCTGCTGTTGGGCAGCTGGGCTCTGTTCTTCCGCCGGCCCAAGG CCTCACTGCCCCGAGTCTTCGTGTTACGAGCTCTGCTCATGGTGCTTGTCTTCCTGCTGGTTATTTCCTA TTGGCTCTTCTACGGTGTGCGCATCTTGGACGCCCGGGAGCGGAGCTACCAGGGCGTGGTTCAGTTTGCC GTTTCTCTAGTGGATGCTTTACTCTTCGTGCACTATCTGGCCGTAGTTCTGCTGGAGCTCCGTCAGCTCC AGCCCCAGTTCACACTCAAGGTCGTGCGATCCACAGATGGGGCCAGCCGCTTCTACAATGTCGGCCATCT CAGCATCCAGCGAGTGGCAGTGTGGATCCTGGAGAAGTATTACCATGACTTCCCTGTCTACAACCCCGCC CTCCTCAACCTGCCCAAGTCCGTCCTGGCCAAGAAAGTGTCTGGCTTCAAGGTGTATTCTCTCGGAGAGG AAAACAGCACCAATAACTCCACGGGCCAATCAAGGGCTGTGATCGCGGCTGCGGCACGGAGGCGCGACAA CAGCCACAATGAGTACTACTACGAGGAAGCCGAGCATGAGCGCAGAGTGCGCAAGCGCAGGGCCAGGCTC GTGGTGGCTGTGGAGGAGGCCTTCACGCACATTAAGCGGCTGCAGGAAGAGGAGCAGAAGAACCCCAGGG AGGTGATGGACCCCCGGGAAGCAGCCCAAGCGATCTTTGCATCCATGGCTCGTGCCATGCAGAAGTACCT TCGCACCACCAAACAGCAGCCTTACCATACCATGGAGAGCATCCTTCAGCACCTGGAGTTCTGCATTACC CACGACATGACACCCAAGGCCTTCCTGGAGCGATATTTGGCTGCTGGACCCACCATCCAGTACCACAAGG AACGTTGGCTGGCCAAACAGTGGACCTTGGTGAGCGAGGAGCCGGTGACCAATGGGCTTAAGGATGGCAT CGTGTTCCTCTTGAAGCGCCAGGACTTCAGCTTGGTAGTGAGCACCAAGAAGGTGCCCTTCTTCAAACTC TCTGAGGAATTTGTGGATCCCAAGTCACATAAGTTCGTCATGCGGCTGCAGTCGGAGACCTCTGTGTGAC TTTTGCAGCAGCCGCGGAGGAGGGATGTGGGGGGTTCCTGCGGAGTGGGAGGGGCTTGGTTCTCTGGCCC TGACACATTTCTGCCAGTCCTACTTCCTCTTGCTCTTGTTTGTTTTTGTTTTTGTTTTGTTTTGTTTTGT TTTTTTTTTTTACTTGAATTAACTTATCCTGTACCCAGTCTCCCCTCTTCCTCAGTTTTTCCCATCTGGA AATCTGGAGATAAATCTTGCTTTAACAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC052195
Insert Size	1566 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC052195, AAH52195
RefSeq Size	2363 bp
RefSeq ORF	1566 bp
Locus ID	93840
Gene Summary	Involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. Required for cell surface localization of FZD3 and FZD6 in the inner ear (PubMed:16495441).[UniProtKB/Swiss-Prot Function]

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