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Fto (BC057008) Mouse Untagged Clone
CAT＃: MC206052
Fto (untagged) - Mouse fat mass and obesity associated (cDNA clone MGC:67044 IMAGE:5708558), (10ug)
CNY 3,744.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AW743446; mKIAA1752 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC057008
GCGGCTTTAGTAGCAGCATGAAGCGCGTCCAGACCGCGGAGGAACGAGAGCGGGAAGCTAAGAAACTGAG GCTCCTTGAGGAGCTTGAAGACACTTGGCTTCCTTACCTGACCCCCAAAGATGATGAGTTCTATCAGCAG TGGCAGCTGAAATACCCTAAACTGGTTTTCCGAGAGGCCGGCAGCATACCAGAGGAGCTGCATAAGGAGG TCCCCGAGGCCTTTCTCACACTGCATAAGCATGGCTGCTTGTTTCGGGACGTGGTGAGGATCCAAGGCAA AGATGTGCTCACCCCAGTGTCTCGCATCCTCATCGGGGACCCAGGCTGCACCTACAAGTACTTGAACACC AGACTCTTCACGGTGCCCTGGCCCGTGAAGGGCTGCACGGTCAAGTACACAGAGGCTGAGATCGCCGCTG CATGTCAGACCTTCCTAAAGCTCAATGACTACCTCCAGGTGGAGACCATCCAGGCCTTGGAAGAACTGGC TGTCAGAGAGAAGGCCAATGAAGACGCTGTGCCACTGTGCATGGCAGAGTTCCCCAGGGCCGGCGTGGGG CCGTCCTGCGATGATGAAGTGGACCTTAAGAGCAGAGCAGCCTACAACGTGACTTTGCTAAACTTCATGG ATCCTCAGAAGATGCCCTACTTGAAAGAGGAGCCCTATTTCGGCATGGGGAAGATGGCGGTGAGCTGGCA TCACGATGAGAACCTGGTGGACAGGTCAGCCGTGGCAGTGTACAGCTATAGCTGCGAAGGCTCTGAGGAT GAAAGTGAGGACGAGTCCAGCTTCGAAGGCAGAGATCCTGATACTTGGCATGTTGGTTTTAAGATCTCTT GGGACATCGAGACACCAGGATTAACAATCCCTCTTCACCAGGGAGACTGCTATTTCATGCTGGATGACCT CAATGCCACCCACCAGCACTGTGTTTTGGCTGGCTCACAGCCTCGGTTTAGTTCCACTCACCGTGTGGCA GAGTGCTCAACAGGCACCTTGGATTATATCTTAGAACGCTGTCAGTTGGCGCTGCAGAATGTCCTCAATG ACTCAGACGATGGCGACGTCTCGTTGAAATCCTTTGATCCTGCAGTTTTGAAACAAGGAGAGGAAATCCA TAATGAGGTGGAGTTTGAGTGGCTGAGGCAGTTCTGGTTTCAAGGCAATCGATACAAACTTTGCACCGAT TGGTGGTGTGAGCCCATGACTCACCTGGAGGGGCTGTGGAAGAAGATGGAGAGCATGACAAATGCGGTGC TCCGTGAAGTTAAAAGAGAGGGGCTCCCGGTGGAACAAAGGAGTGAGATTCTGTCTGCCATCCTGGTCCC GCTCACCGTGCGCCAGAACCTGAGGAAGGAGTGGCATGCCAGGTGCCAGTCCCGAGTCGTCCGGACTTTA CCAGTACAGCAGAAACCAGACTGCCGGCCATATTGGGAGAAGGATGACCCTTCCATGCCTCTGCCCTTTG ACCTCACAGACGTGGTTTCCGAGCTCAGAGGCCAGCTGCTGGAAGCAAGATCCTAGAGGAAGCACAAGTC TTAGGTGGAGAAGGAAAAGATATCCTTGGCTTTCTTCCTCAAACCTTGGACAAGGGTAGTGTAGACTCCT CTCAACCCAGTGGTTATAGGACCTGGGTTCCAATTCAAAACAGCTAGAGAACAAAGCCCACAGATTCTGA AGTGTTGAAACGTTACTGTGTTTTAGTCTGACTTGGTGTTAAATTAGAGCACCCCTCCCTCCCCCCAAAT ATTCTTCACTCACCACCCCCCACACACACCTGGAGCCCTTTTGTCTCCCAAGGCAGCAGTAGAGATGGGC TAAGCCTGTGACAAGCCTCACCTGCTCTCCTCTTTGGCTGGCCACCTCAAAGACCCGGAAGTAGGCCTGG CCATAGGGTGACAGAACTAGAGACATCCATCCCAGCTTCTCACCGTGGCTTCTAGTGGTTAGACCATCCA AGATGGCAGCCAGTGTCTCACCGCGGCTGCACAGATATAAATTCAGCTCAGTTGTCATTAAAACTCCATT CTTCAGTTGCCCTGGCGCATGCTGATGACTGCTGATTTAGACGACACATACAGATGTTTATGTGTTCCAC ATCTGTGAGCGAGCCTTCCTTCTCCCAGCCTTCTGTAGTGGCTAAAACATGGAGGAAAACAGTGAAAGGC AGTCACCACTGTCACCTCTTTCCTCCTCTGAGAGGGAAGGGGCCATGAGCCCTGTGCACTGCACACAGGA AGAGGAAGGAGCTACAAGGAGACAGCCAAACCCTGTCTTTGCGGGGCTTCCCTGATCCAGTGACAGAGAC CAGCCCTGGGACCACTGTTCTGTGGAGCTAGTGTTTCTCCTAGAATTCCCCACTCATAGATACTGACAGG TCGTATGGGGGCTTGAAGCACCCAGTTCCCAACCCCGTGATGTCAGAGCGTCAGAGAGAGCATGGACTCC GATTTCAAAGTGCCAGGAGTAAGACCCACCGCAGCACTCACTCCATGACCTTTGCATGTCCTTGGGTCTC AGTTTTCTCATCTGTATGATTTTGGGAGGTACCCAAAATGCCAACTCCTAATGTGAAAATGACGTGATAT GACAGAGCTACAGATCTAGAGTAATTTCTAGCATACCGCTGAATATCCGATATGTTCCAGTGTCGTTACG TGAGCTGACCTGGAAGAGGCCGTCTTCCTCCCCCCCCCCCCCCCGGGTCTGCAGTCCTTTGCCGTCTTGT GTCTCGTGGCCCACATCTTTCCCTAGTGACCTGAGGCCTTTTGGTGGCTGAAAGGTTGACAACAGTTAGC CCATGATTCTCTTGTATTGGCCGAGACCTGCAATTGGAAGGACACAACATAGTTGTGGTTTTTGCTGCAT CCAGGATTCCAGTTAAGGTGCCTTCCTGCCTAGAATCCACCATAGAAGCTGCACTCCACCAGCCCCAAGG GCTTCCTGGTGCAGGGACCCAAGGCTCTCAGACTTTGATTTGTTCCCCTTTGTCTCTGAGCCAGGACTTC CTCATCAGTAGAGAGTGAGGTACATTCTCACGGGGTAAAATGGAACAACCTAAGTAACGGTATAGGTGGC TTCATATGTCACAGGCTGGCCCGTCTCCCCATCCCCAGCCATCTGGCTCTGTGGTTTATTAGGCCCCGTG ATATTTCTTTGTTCTATTTAATTGGGTGCCAGAATTAAAAATTGAGAGATTCCACATAAAATAAAAATGT CTGCATTCTCTTTAGAAGCCAGAATACCTGTGAGCCCCTGGGTCTCCATAATCGCATGGCGGTGGCTTGG CAATGCCTGTGACCTTTGTGCTCTGCTGTGACCCTGTTGCTCCCTGTCACCATTTTGTAAACCTGCGTCC GTCCCAGCAGCACTTTCTAGAGCTGCTTGCTCGCATCCTTGGTAGAAAGCAGTAGCGTTGAGTGCTCAGT CCCTGCTCCTCCCATTGGTCTAAGTTCATGGTCTGCTTGTATTGCTGTGCTGTGTGCATTTGTTCATACA CAATAAAGAATTAAACGATGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | BC057008 |
| Insert Size | 1509 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC057008, AAH57008 |
| RefSeq Size | 3552 bp |
| RefSeq ORF | 1509 bp |
| Locus ID | 26383 |
| Gene Summary | RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipogenesis and energy homeostasis (PubMed:17991826, PubMed:18775698, PubMed:28002401). Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:28002401). M6A demethylation by FTO affects mRNA expression and stability (By similarity). Also able to demethylate m6A in U6 small nuclear RNA (snRNA) (By similarity). Mediates demethylation of N(6),2'-O-dimethyladenosine cap (m6A(m)), by demethylating the N(6)-methyladenosine at the second transcribed position of mRNAs and U6 snRNA (PubMed:28002401). Demethylation of m6A(m) in the 5'-cap by FTO affects mRNA stability by promoting susceptibility to decapping (By similarity). Also acts as a tRNA demethylase by removing N(1)-methyladenine from various tRNAs (By similarity). Has no activity towards 1-methylguanine (By similarity). Has no detectable activity towards double-stranded DNA (By similarity). Also able to repair alkylated DNA and RNA by oxidative demethylation: demethylates single-stranded RNA containing 3-methyluracil, single-stranded DNA containing 3-methylthymine and has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine (PubMed:17991826, PubMed:18775698). Ability to repair alkylated DNA and RNA is however unsure in vivo (PubMed:17991826, PubMed:18775698). Involved in the regulation of fat mass, adipogenesis and body weight, thereby contributing to the regulation of body size and body fat accumulation (PubMed:19234441, PubMed:19680540, PubMed:21076408, PubMed:23817550, PubMed:23300482). Involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed:19234441, PubMed:19680540). Regulates activity of the dopaminergic midbrain circuitry via its ability to demethylate m6A in mRNAs (PubMed:23817550).[UniProtKB/Swiss-Prot Function] |
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