Rpn2 (BC046806) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1300012C06Rik; AV261018; Rpn-2
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC046806 GCGCGGGGGAGAGGACGGCTTGGCAAGCAGGACCCGTGAGTGAGGTTCGCACGCCGCGACTCAACTGCCC CACACTGCGGCTGCCGGGCACGGCCCTGCCAAGCGGGACCGCCGCGACTGACCTTTGCCGGGAGCCCATT CTGGCAAGTGGCGTGTGCCGCGGGTCCCTGGCGTCCCCTCCGGCGCGCTCTTGGAGCCACTTCCCGGAGC GGAAGTCTGCCTGCTCGGGCTCCGGTGGGACCTGCTCGGAGGAATGGCGCCGCCGGGTTCAAGCGCTGTC TTCCTGTTGGCCCTGACAATCACTGCCAGCGTCCAGGCTCTGACCCCCACCCACTACCTCACCAAGCAGG ATGTGGAAAGGCTGAAGGCCTCACTGGACCGCCCCTTCACAGATTTGGAGTCTGCCTTCTACTCCATCGT GGGACTCAGCAGCCTTGGGGTACAGGTGCCAGATGTCAAGAAAGCGTGTACCTTCATCAAGTCCAACCTT GATCCCAGCAACGTGGATTCTCTCTTCTATGCAGCCCAGTCCAGCCAGGTCCTCTCAGGCTGTGAGATAT CTGTTTCAAATGAGACCAAAGAGCTCCTGCTGGCAGCAGTGAGTGAAGACTCCCCCATTGCCCAGATCTA CCATGCAGTTGCAGCACTCAGCGGGTTTGGGCTTCCCTTGGCATCCAATGAAGCCCTCGGTGCCCTTACC GCTCGCCTCGGCAAGGAAGAGACTGTGCTAGCAACGGTCCAGGCTCTGCAGACAGCGTCCCACCTCTCCC AGCAGGCTGACCTGAGGAACATTGTAGAGGAGATTGAGGACCTTGTTGCTCGGCTGGATGAACTAGGGGG TGTGTATCTGCAGTTTGAGGAAGGACTGGAGCTCACGGCGCTGTTTGTTGCTGCCACCTACAAGCTCATG GACCACGTGGGGACCGAACCGTCCATGAAGGAGGATCAGGTTATCCAGCTCATGAACACAATCTTCAGCA AGAAGAACTTTGAGTCCCTCTCAGAAGCCTTCAGTGTGGCCTCTGCTGCTGCTGCATTGTCCCAGAATCG CTACCACGTACCAGTGGTGGTTGTGCCCGAGGGCTCTACTTCTGACACTCAAGAACAGGCTATCCTGAGG TTGCAGGTCAGCAATGTTTTGTCTCAGCCTCTGGCTCAAGCTGCAGTGAAGCTGGAGCATGCTAAGTCGG CGGCTACCAGGGCTACCGTCCTGCAGAAGACGCCCTTTTCGCTTGTGGGGAATGTTTTTGAGCTAAACTT CAAGAATGTTAAACTTTCCAGTGGCTACTATGACTTCTCTGTCCGAGTTGAAGGTGACAGCCGTTACATT GCAAACACTGTAGAGCTTCGAGTCAAGATCTCCACTGAAGTTGGCATCACAAATGTTGATCTTTCCACTG TGGATAAGGATCAGAGCATTGCACCCAAAACTACCCGGGTGACCTACCCAGCCAAAGCCAAGGGTACATT CATCGCAGACAGCCATCAGAACTTTGCCCTGTTTTTCCAGCTGGTAGATGTGAACACCGGTGCGGAGCTC ACCCCTCACCAGACGTTTGTTCGACTTCATAACCAGAAGACTGGCCAGGAAGTGGTGTTTGTTGCTGAGC CAGATAACAAGAATGTATATAAGTTTGAACTGGACACCTCTGAAAGGAAGATTGAATTTGACTCTGCCTC TGGCACCTACACACTCTACTTAATCATCGGGGATGCCACATTGAAGAACCCAATCCTCTGGAACGTGGCT GACGTGGTTATCAAGTTCCCTGAGGAGGAAGCCCCCTCGACTGTGCTGTCCCAGAGCCTGTTTACCCCAA AACAGGAAATTCAGCACCTGTTCCGAGAGCCTGAGAAGAGGCCCCCCACAGTGGTGTCCAATACATTCAC GGCCCTCATCCTCTCGCCCTTGCTCCTGCTCTTTGCACTGTGGATCCGGATTGGAGCCAATGTCTCCAAC TTCACATTTGCTCCTAGCACAGTTATCTTCCACCTGGGACATGCTGCGATGCTGGGCCTCATGTATATCT ACTGGACTCAGCTCAACATGTTCCAGACCCTCAAGTACCTGGCTGTCCTGGGCACTGTGACGTTTCTGGC TGGCAACCGAATGCTGGCCCAGCATGCAGTTAAGAGAACAGCACATTAGTTCCAGAAGAAGTCTGAAGAC CCTGAAGGCTGAAATGAACATTTAACAAGGAGTGGGGACAGTTCATGGTGTGAAAGGAAGAACTGGGGAC ACAACAGGAGGGAGGAGATGCCTGTTCGTTAAAAAGAGAAAAGCTGAGCTGTGCTTACATGTTTACTTGT TTCTCACTTTTTGCTTCACAGAGCAGACATGTTTGGGCCCAGATTGTCTGTCCCTTTGTCTTGATGCCTG GCAAGATGCTGTGAATATCCCAGGCTACCCAGATGTTGTATTTGAAAAGTTGAAATCTGTAATTCATCAT CAGCTTTGGAATAAAGAGAATGGTGGAAAAAAAAAAAAAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC046806
Insert Size	1896 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC046806, AAH46806
RefSeq Size	2494 bp
RefSeq ORF	1896 bp
Locus ID	20014
Gene Summary	Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.[UniProtKB/Swiss-Prot Function]

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