Rpn2 (BC046806) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1300012C06Rik; AV261018; Rpn-2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209608 representing BC046806 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCCGCCGGGTTCAAGCGCTGTCTTCCTGTTGGCCCTGACAATCACTGCCAGCGTCCAGGCTCTGA CCCCCACCCACTACCTCACCAAGCAGGATGTGGAAAGGCTGAAGGCCTCACTGGACCGCCCCTTCACAGA TTTGGAGTCTGCCTTCTACTCCATCGTGGGACTCAGCAGCCTTGGGGTACAGGTGCCAGATGTCAAGAAA GCGTGTACCTTCATCAAGTCCAACCTTGATCCCAGCAACGTGGATTCTCTCTTCTATGCAGCCCAGTCCA GCCAGGTCCTCTCAGGCTGTGAGATATCTGTTTCAAATGAGACCAAAGAGCTCCTGCTGGCAGCAGTGAG TGAAGACTCCCCCATTGCCCAGATCTACCATGCAGTTGCAGCACTCAGCGGGTTTGGGCTTCCCTTGGCA TCCAATGAAGCCCTCGGTGCCCTTACCGCTCGCCTCGGCAAGGAAGAGACTGTGCTAGCAACGGTCCAGG CTCTGCAGACAGCGTCCCACCTCTCCCAGCAGGCTGACCTGAGGAACATTGTAGAGGAGATTGAGGACCT TGTTGCTCGGCTGGATGAACTAGGGGGTGTGTATCTGCAGTTTGAGGAAGGACTGGAGCTCACGGCGCTG TTTGTTGCTGCCACCTACAAGCTCATGGACCACGTGGGGACCGAACCGTCCATGAAGGAGGATCAGGTTA TCCAGCTCATGAACACAATCTTCAGCAAGAAGAACTTTGAGTCCCTCTCAGAAGCCTTCAGTGTGGCCTC TGCTGCTGCTGCATTGTCCCAGAATCGCTACCACGTACCAGTGGTGGTTGTGCCCGAGGGCTCTACTTCT GACACTCAAGAACAGGCTATCCTGAGGTTGCAGGTCAGCAATGTTTTGTCTCAGCCTCTGGCTCAAGCTG CAGTGAAGCTGGAGCATGCTAAGTCGGCGGCTACCAGGGCTACCGTCCTGCAGAAGACGCCCTTTTCGCT TGTGGGGAATGTTTTTGAGCTAAACTTCAAGAATGTTAAACTTTCCAGTGGCTACTATGACTTCTCTGTC CGAGTTGAAGGTGACAGCCGTTACATTGCAAACACTGTAGAGCTTCGAGTCAAGATCTCCACTGAAGTTG GCATCACAAATGTTGATCTTTCCACTGTGGATAAGGATCAGAGCATTGCACCCAAAACTACCCGGGTGAC CTACCCAGCCAAAGCCAAGGGTACATTCATCGCAGACAGCCATCAGAACTTTGCCCTGTTTTTCCAGCTG GTAGATGTGAACACCGGTGCGGAGCTCACCCCTCACCAGACGTTTGTTCGACTTCATAACCAGAAGACTG GCCAGGAAGTGGTGTTTGTTGCTGAGCCAGATAACAAGAATGTATATAAGTTTGAACTGGACACCTCTGA AAGGAAGATTGAATTTGACTCTGCCTCTGGCACCTACACACTCTACTTAATCATCGGGGATGCCACATTG AAGAACCCAATCCTCTGGAACGTGGCTGACGTGGTTATCAAGTTCCCTGAGGAGGAAGCCCCCTCGACTG TGCTGTCCCAGAGCCTGTTTACCCCAAAACAGGAAATTCAGCACCTGTTCCGAGAGCCTGAGAAGAGGCC CCCCACAGTGGTGTCCAATACATTCACGGCCCTCATCCTCTCGCCCTTGCTCCTGCTCTTTGCACTGTGG ATCCGGATTGGAGCCAATGTCTCCAACTTCACATTTGCTCCTAGCACAGTTATCTTCCACCTGGGACATG CTGCGATGCTGGGCCTCATGTATATCTACTGGACTCAGCTCAACATGTTCCAGACCCTCAAGTACCTGGC TGTCCTGGGCACTGTGACGTTTCTGGCTGGCAACCGAATGCTGGCCCAGCATGCAGTTAAGAGAACAGCA CAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209608 representing BC046806 Red=Cloning site Green=Tags(s) MAPPGSSAVFLLALTITASVQALTPTHYLTKQDVERLKASLDRPFTDLESAFYSIVGLSSLGVQVPDVKK ACTFIKSNLDPSNVDSLFYAAQSSQVLSGCEISVSNETKELLLAAVSEDSPIAQIYHAVAALSGFGLPLA SNEALGALTARLGKEETVLATVQALQTASHLSQQADLRNIVEEIEDLVARLDELGGVYLQFEEGLELTAL FVAATYKLMDHVGTEPSMKEDQVIQLMNTIFSKKNFESLSEAFSVASAAAALSQNRYHVPVVVVPEGSTS DTQEQAILRLQVSNVLSQPLAQAAVKLEHAKSAATRATVLQKTPFSLVGNVFELNFKNVKLSSGYYDFSV RVEGDSRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQL VDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDNKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATL KNPILWNVADVVIKFPEEEAPSTVLSQSLFTPKQEIQHLFREPEKRPPTVVSNTFTALILSPLLLLFALW IRIGANVSNFTFAPSTVIFHLGHAAMLGLMYIYWTQLNMFQTLKYLAVLGTVTFLAGNRMLAQHAVKRTA H TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC046806
ORF Size	1893 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC046806, AAH46806
RefSeq Size	2494 bp
RefSeq ORF	1895 bp
Locus ID	20014
Gene Summary	Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.[UniProtKB/Swiss-Prot Function]

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