Sirt7 (BC026403) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MGC31235, MGC37560
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC026403 AGCCGGTGGCGGTCTGAGCCGCTCGGAGCGCAAAGCTGCTGAGCGGGTCCGGAGGCTGCGGGAGGAGCAG CAGCGGGAGCGCCTCCGCCAGGTGTCACGCATCCTGAGGAAGGCGGCTGCAGAGCGCAGCGCGGAGGAGG GCCGGCTCTTGGCCGAGAGCGAGGATCTGGTGACCGAGCTGCAGGGTCGAAGTCGGCGGCGTGAGGGCCT CAAGCGCCGCCAGGAGGAGGTGTGTGATGACCCGGAGGAGCTGCGGAGGAAGGTCCGCGAACTGGCCGGA GCTGTCCGAAGTGCCAGGCACTTGGTTGTCTACACGGGCGCTGGAATCAGCACAGCAGCTTCTATCCCAG ATTATCGGGGTCCTAATGGAGTATGGACACTGCTTCAGAAAGGAAGGCCTGTGAGTGCTGCCGACCTAAG CGAAGCGGAGCCTACCCTCACCCACATGAGCATCACCCGTTTGCATGAGCAAAAGCTGGTGCAACACGTG GTGTCTCAGAACTGTGATGGGCTCCACCTGCGGAGTGGGTTGCCCCGGACCGCCATCTCAGAGCTCCATG GGAATATGTATATTGAAGTCTGCACCTCCTGCATCCCTAACAGAGAGTATGTACGAGTGTTTGATGTGAC TGAGCGTACTGCCCTTCACCGACACCTGACAGGCCGGACCTGCCACAAGTGCGGGACCCAGCTTCGGGAT ACCATTGTGCACTTTGGGGAGAGGGGGACATTAGGGCAGCCTCTGAACTGGGAGGCAGCGACCGAGGCTG CTAGCAAAGCAGACACAATCCTGTGTTTAGGGTCCAGCTTGAAGGTACTAAAGAAATATCCCCGCCTCTG GTGCATGACGAAGCCTCCAAGCCGTCGACCCAAACTCTACATCGTGAACCTGCAGTGGACCCCGAAGGAT GACTGGGCTGCCCTGAAACTCCATGGGAAGTGTGATGATGTCATGCAACTCCTCATGAATGAACTGGGCC TGGAGATTCCTGTCTACAACCGGTGGCAGGATCCAATCTTCTCCTTGGCGACCCCCCTCCGTGCTGGTGA AGAAGGCAGCCACAGTAGGAAGTCACTATGCAGAAGCAGAGAAGAAGCCCCACCTGGGGACCAGAGTGAC CCCCTTGCCTCAGCTCCCCCTATCCTAGGAGGCTGGTTTGGCAGGGGTTGTGCCAAGCGTGCAAAAAGGA AGAAAGTGGCATAGCCCGGAGTTGACAAAGAACAGTTGGCACTTTGCAGATGGCTTGACCCTTGTTCAGG GGCTCCGCCCGTGTCGCCGTGAAGGCGAGAGTGCCCCCTCCGGCTCTAGTGGGTGCCGGCTTCGCGGTGA CGGTCGTCTTTAGCCATTTGTCCTTGTGGAAAGGCTCTCTTGCACTGCTGTGACTGCCCCGCTATGGGCT TGATCTTCAAGGACACCTCCCTCCTGTTCACCCCTGTACAAAGGTGGCAGCAAGGCCTCTGTGAGAAGAG CTCCGGCCCTTCCCAGGCCTGGCCAGCAGGAGAGTCCACCACTGCCTTACCTCACTCTCCAGGCTAGTCT CAGGGCCTCCCTCTTTCTACTCCTTATCACGTACAAGTGTTCACTTTATAGAAGCCTTTTTCTGTATTGG GTATGCGGCACAGGGAGAATCTAACTAAGCCTCCAGTCTGAGCAAGTCTTATTAAACATCTCTCAATTGC TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC026403
Insert Size	759 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC026403, AAH26403
RefSeq Size	1712 bp
RefSeq ORF	759 bp
Locus ID	209011
Gene Summary	NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions (By similarity). Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis. Promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation.[UniProtKB/Swiss-Prot Function]

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