Il1a (BC003727) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Il-1a
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003727 GCCACTGGTGTTCTCTTACAGAAATCAAGATGGCCAAAGTTCCTGACTTGTTTGAAGACCTAAAGAACTG TTACAGTGAAAATGAAGACTACAGTTCTGCCATTGACCATCTCTCTCTGAATCAGAAATCCTTCTATGAT GCAAGCTATGGCTCACTTCATGAGACTTGCACAGACCAGTTTGTATCTCTGAGAACCTCTGAAACGTCAA AGATGTCCAACTTCACCTTCAAGGAGAGCCGGGTGACAGTATCAGCAACGTCAAGCAACGGGAAGATTCT GAAGAAGAGACGGCTGAGTTTCAGTGAGACCTTCACTGAAGATGACCTGCAGTCCATAACCCATGATCTG GAAGAGACCATCCAACCCAGATCAGCACCTTACACCTACCAGAGTGATTTGAGATACAAACTGATGAAGC TCGTCAGGCAGAAGTTTGTCATGAATGATTCCCTCAACCAAACTATATATCAGGATGTGGACAAACACTA TCTCAGCACCACTTGGTTAAATGACCTGCAACAGGAAGTAAAATTTGACATGTATGCCTACTCGTCGGGA GGAGATGACTCTAAATATCCTGTTACTCTAAAAATCTCAGATTCACAACTGTTCGTGAGCGCTCAAGGAG AAGACCAGCCCGTGTTGCTGAAGGAGTTGCCAGAAACACCAAAACTCATCACAGGTAGTGAGACCGACCT CATTTTCTTCTGGAAAAGTATCAACTCTAAGAACTACTTCACATCAGCTGCTTATCCAGAGCTGTTTATT GCCACCAAAGAACAAAGTCGGGTGCACCTGGCACGGGGACTGCCCTCTATGACAGACTTCCAGATATCAT AAAAGCAGCCTTATTTCGGGAGTCTATTCACTTGGGAAGTGCTGACAGTCTGTATGTACCATGTACAGGA ACCTTCCTCACCCTGAGTCACTTGCACAGCATGTGCTGAGTCTCTGTAATTCTAAATGAATGTTTACCCT CTTTGTAAGAGAAGAGCAAACCCTAGTGGAGCCACCCCGACATATGATACTATCTGTTATTTTAAAGAGT ACCCTATAGTTTGCTCAGTACTAATCATTTTAATTACTATTCTGCATGGCATTCTTAGGAGGATCAAAAA GACTCTACACATATTACAGATGGGTTAACAAAGGGATAAAACAACTGAAAAGCACACTCAATGCATTTGG AATATAAATTCACAGACCAATCTCACTGTGCACCTTCGGCTTCAAAATGCCAGTTGAGTAGGATAAAGGT ATAAGAACTTAATGCTGTCATTTTCAAAAGGAAGGGGACAATAGCTACATCTTTCCTACCTCAGTGGGTT TTACTCCAGTGAGATCATTTGGATGAAATCCTCCTGTAACAGACCTCAAGAAGGAGACAGACTGTTGAAT GTTATTTTTAAGTTATTTTATATATGTATTTATAAATATATTTATGATAATTATATTATTTATGGAACAT CCTTAAATCCTCTGAGCTTGACAGGCATCCTCACAGCAGGATTTTCTAGGTGGTCAGTTAGATATAGTTT CCTCTAGAGCACCATGCTACAGACTTTACACTTTTTCCACAGCCACGAAGCTCTCTGTACATTCCTGTAC TTGGGAGCCCTTTCATCATGATCTTAATCTGTACTGTTTACTTTGTTCATCTAAAATGATAATTGAGTCA GTCTTTTTCCCTCCCATCCTTAAAGCTGTCTGGGTATTCTTACATCATTCAGTCTCACCTGTAACTAACA CCAACCATCTAAAGATGGAAAGAGCTTAACTGTGACAACCACATCACTGTTACCTGAAGTTTCTTTTCTA GAATGTAATCAGTGTTTCCCCTGGATTCCAATATTTTTTTCAAACCACAGTATCATGTAACTATCAACAA TAACAATCAACTCATTATTATTAATCATAATTAAATAAAACAAGTTTGAGCTGAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC003727
Insert Size	813 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003727, AAH03727
RefSeq Size	1973 bp
RefSeq ORF	813 bp
Locus ID	16175
Gene Summary	Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.