Zfp36l1 (BC016621) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Brf1, TIS11b, ERF1, cMG1, Berg36
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC016621, the custom clone sequence may differ by one or more nucleotides ATGACCACCACCCTCGTGTCCGCCACCATTTTTGACTTGAGCGAAGTTTTATGCAAGGGTAACAAGATGC TCAACTACAGCACTCCCAGCGCTGGGGGCTGCCTGCTGGACAGGAAGGCAGTGGGCACCCCTGCTGGCGG GGGCTTCCCTCGCAGGCACTCGGTCACTCTGCCCAGCTCCAAGTTCCATCAGAACCAGCTTCTCAGCAGC CTTAAGGGTGAGCCGGCCCCGTCCCTGAGCTCACGCGACAGCCGCTTTCGAGACCGCTCTTTCTCCGAAG GGGGCGAGCGGCTGCTGCCCACCCAGAAGCAGCCTGGGAGCGGCCAGGTCAACTCCAGCCGCTACAAGAC GGAGCTGTGCCGTCCCTTCGAAGAAAACGGTGCCTGTAAGTACGGGGACAAGTCCCAGTTCGCGCATGGC ATCCACGAGCTCCGCAGCCTGACCCGCCACCCCAAGTACAAGACGGAGCTGTGCCGCACCTTCCACACCA TCGGCTTTTGCCCGTACGGGCCCCGCTGCCACTTCATTCATAACGCCGAGGAGCGACGCGCCCTGGCGGG GGGCCGAGACCTCTCCGCTGACCGTCCCCGCCTCCAGCATAGCTTTAGCTTTGCTGGGTTTCCCAGTGCC GCTGCCACCGCCGCTGCCACGGGGCTGCTGGACAGCCCCACATCCATCACCCCACCCCCTATCCTGAGCG CCGATGACCTCTTGGGCTCACCTACTCTGCCCGATGGCACCAATAACCCCTTCGCCTTTTCCAGCCAGGA GCTGGCGAGCCTCTTTGCTCCTAGCATGGGGCTGCCTGGGGGAGGCTCCCCCACCACTTTCCTCTTCCGG CCCATGTCCGAATCCCCTCACATGTTTGACTCTCCCCCCAGCCCTCAGGATTCTCTCTCGGACCACGAGG GCTATCTGAGCAGCTCCAGCTCCAGCCACAGTGGCTCAGACTCCCCTACCTTGGACAACTCAAGACGCCT GCCCATTTTCAGCAGACTCTCCATCTCAGATGACTAA
Restriction Sites	EcoRI-NotI
ACCN	BC016621
Insert Size	1017 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016621, AAH16621
RefSeq Size	1967 bp
RefSeq ORF	1017 bp
Locus ID	12192
Gene Summary	Zinc-finger RNA-binding protein that destabilizes several cytoplasmic AU-rich element (ARE)-containing mRNA transcripts by promoting their poly(A) tail removal or deadenylation, and hence provide a mechanism for attenuating protein synthesis (PubMed:22701344, PubMed:24700863, PubMed:24733888, PubMed:27102483). Acts as a 3'-untranslated region (UTR) ARE mRNA-binding adapter protein to communicate signaling events to the mRNA decay machinery (By similarity). Functions by recruiting the CCR4-NOT deadenylating complex and components of the cytoplasmic RNA decay machinery to the bound ARE-containing mRNAs, and hence promotes ARE-mediated mRNA deadenylation and decay processes (By similarity). Induces also the degradation of ARE-containing mRNAs even in absence of poly(A) tail (By similarity). Binds to 3' UTR ARE of numerous mRNAs (PubMed:22701344, PubMed:24700863, PubMed:24733888). Positively regulates early adipogenesis by promoting ARE-mediated mRNA decay of immediate early genes (IEGs) (PubMed:22701344). Promotes ARE-mediated mRNA decay of mineralocorticoid receptor NR3C2 mRNA in response to hypertonic stress (PubMed:24700863). Negatively regulates hematopoietic/erythroid cell differentiation by promoting ARE-mediated mRNA decay of the transcription factor STAT5B mRNA (By similarity). Positively regulates monocyte/macrophage cell differentiation by promoting ARE-mediated mRNA decay of the cyclin-dependent kinase CDK6 mRNA (By similarity). Promotes degradation of ARE-containing pluripotency-associated mRNAs in embryonic stem cells (ESCs), such as NANOG, through a fibroblast growth factor (FGF)-induced MAPK-dependent signaling pathway, and hence attenuates ESC self-renewal and positively regulates mesendoderm differentiation (PubMed:24733888). May play a role in mediating pro-apoptotic effects in malignant B-cells by promoting ARE-mediated mRNA decay of BCL2 mRNA (By similarity). In association with ZFP36L2 maintains quiescence on developing B lymphocytes by promoting ARE-mediated decay of several mRNAs encoding cell cycle regulators that help B cells progress through the cell cycle, and hence ensuring accurate variable-diversity-joining (VDJ) recombination and functional immune cell formation (PubMed:27102483). Together with ZFP36L2 is also necessary for thymocyte development and prevention of T-cell acute lymphoblastic leukemia (T-ALL) transformation by promoting ARE-mediated mRNA decay of the oncogenic transcription factor NOTCH1 mRNA (PubMed:20622884). Involved in the delivery of target ARE-mRNAs to processing bodies (PBs) (By similarity). In addition to its cytosolic mRNA-decay function, plays a role in the regulation of nuclear mRNA 3'-end processing; modulates mRNA 3'-end maturation efficiency of the DLL4 mRNA through binding with an ARE embedded in a weak noncanonical polyadenylation (poly(A)) signal in endothelial cells (By similarity). Also involved in the regulation of stress granule (SG) and P-body (PB) formation and fusion (By similarity). Plays a role in vasculogenesis and endocardial development (PubMed:15226444, PubMed:17013884). Involved in the regulation of keratinocyte proliferation, differentiation and apoptosis (By similarity). Plays a role in myoblast cell differentiation (PubMed:17889962).[UniProtKB/Swiss-Prot Function]

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