Hs3st1 (BC009133) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3-OST
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC009133 CTGCGGTCACCGCCGGGTCTCTCTCGGTGAGTGCCTGTGTCCCTTCACCGCTCGCCCGTCCCGGGGTGAG TGTGAATTTGCTCCAAAGGGCGCAGGTACTTGGCGGCGGTGCTGCCCATGGCCCGGCCGGAGAGAGGATT GAGGGCGCCGGACCGGCTGGACGGGTACGCGACTCAGTAATTGGCAACTGGAGATACTCATGTTCCAGTA ATCAAGAGGGAGCCTTGCTGCTACTTCATGATCCAGGCGCGTGTGGCCCAGTGAAGTCCCTGAGCTGTAC AGCATGACCTTGCTGCTCCTGGGTGCGGTGCTGCTGGTGGCCCAGCCCCAGCTTGTGCATTCCCACCCGG CTGCTCCTGGCCCGGGGCTCAAACAGCAGGAGCTTCTGAGGAAGGTGATTATTCTCCCAGAGGACACCGG AGAAGGCACAGCATCCAATGGTTCCACACAGCAGCTGCCACAGACCATCATCATTGGGGTGCGCAAGGGT GGTACCCGAGCCCTGCTAGAGATGCTCAGCCTGCATCCTGATGTTGCTGCAGCTGAAAACGAGGTCCATT TCTTTGACTGGGAGGAGCATTACAGCCAAGGCCTGGGCTGGTACCTCACCCAGATGCCCTTCTCCTCCCC TCACCAGCTCACCGTGGAGAAGACACCCGCCTATTTCACTTCGCCCAAAGTGCCTGAGAGAATCCACAGC ATGAACCCCACCATCCGCCTGCTGCTTATCCTGAGGGACCCATCAGAGCGCGTGCTGTCCGACTACACCC AGGTGTTGTACAACCACCTTCAGAAGCACAAGCCCTATCCACCCATTGAGGACCTCCTAATGCGGGACGG TCGGCTGAACCTGGACTACAAGGCTCTCAACCGCAGCCTGTACCATGCACACATGCTGAACTGGCTGCGT TTTTTCCCGTTGGGCCACATCCACATTGTGGATGGCGACCGCCTCATCAGAGACCCTTTCCCTGAGATCC AGAAGGTCGAAAGATTCCTGAAGCTTTCTCCACAGATCAACGCCTCGAACTTCTACTTTAACAAAACCAA GGGCTTCTACTGCCTGCGGGACAGTGGCAAGGACCGCTGCTTACACGAGTCCAAAGGCCGGGCGCACCCC CAGGTGGATCCCAAACTACTTGATAAACTGCACGAATACTTTCATGAGCCAAATAAGAAATTTTTCAAGC TCGTGGGCAGAACATTCGACTGGCACTGATTTGCCGTCTCCTAGGCTCGGGACTTTTCCTGTTGTTAACT TCTGGTGTACATCTGAAGGGGGGAGGAAAATAATTTTAAAAAGGCATTTAAGCTATAATTTATTTGTAAA ACCCACAAATGACTTCTGTACAGTATTAGATTCACAGTTGCCATATATAGTAGTTATATTTTTCTACTTG TTAAATGGAGGGCGTTTTGTATTGTTTTTCATGGTTGTTAACATTGTGTATATGTCTCTATAATATGAAG GAACTTAACTATTGCACTGAAAAAATAAGAGATTTTTTTTTTCTGGAGACCTCTTTTTTTGTTGTTGTTG TTTTAAATATAATTAACCTGCCTCCAATCCAAAATAGCTCTTTGTTTTCACCTCCTTGTCAAATCTATAA TCTTTTTCTGCTTAAAAAAATTTATTGGTATTAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC009133
Insert Size	936 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC009133, AAH09133
RefSeq Size	1657 bp
RefSeq ORF	936 bp
Locus ID	15476
Gene Summary	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.[UniProtKB/Swiss-Prot Function]

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