Darc (BC005583) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	FY, GPD, CCBP1, CD234
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC005583 AGAGAAGTCATTAGCCCTGGGCACTTATCTTGGAGCCACAGCTGCTGACAGAGTCCAGGCCCTGTACTTC TCTGCCCTGAGCCTGCAGTGCCATGGGGAACTGTCTGTATCCGGTGGAAACCCTTTCACTAGACAAGAAT GGAACTCAGTTTACTTTCGACAGCTGGAATTATTCGTTTGAAGATAACTACTCCTATGAACTCTCGAGTG ACTACAGCCTGACACCAGCTGCTCCCTGCTACTCCTGTAATCTGCTTGACAGGTCTTCCCTGCCCTTCTT CATGCTCACCAGTGTCCTGGGCATGCTGGCAAGTGGCAGCATCCTCTTCGCGATTCTCAGACCTTTCTTC CACTGGCAGATTTGCCCCAGCTGGCCCATTCTGGCAGAGTTAGCAGTGGGCAGTGCCCTGTTCAGCATTG CAGTGCCCATCCTGGCACCAGGCTTACACAGCGCCCACAGCACAGCCCTATGCAACCTGGGCTACTGGGT ATGGTATACTTCTGCTTTTGCCCAAGCTCTGTTGATAGGATGCTATGCTTGCCTGAATCCCAGACTGAAT ATTGGTCAACTCCGTGGCTTCACCTTGGGACTCAGTGTGGGACTTTGGGGAGCAGCTGCCCTCTCAGGGC TGCCAGTCGCCCTGGCCAGTGATGTTTACAATGGCTTCTGCACCTTTCCATCCTCCAGAGACATGGAAGC TTTGAAGTACACTCATTATGCCATCTGTTTTACCATCTTCACTGTATTGCCACTGACTCTTTTGGCAGCC AAGGGGCTGAAGATAGCACTGAGCAAGGGGCCTGGCCCCTGGGTTAGTGTCTTGTGGATCTGGTTCATTT TCTGGTGGCCTCATGGGATGGTTCTCATATTTGATGCTTTGGTGAGGTCCAAAACTGTTCTTCTGTATAC ATGTCAATCCCAGAAGATTCTTGATGCGATGCTGAATGTGACAGAAGCCCTGAGTATGCTGCACTGTGTG GCTACCCCACTGCTCCTGGCCCTGTTCTGCCATCAGACCACCCGTAGATCCTTGTCCTCACTCTCCCTCC CTACAAGACAGGCTTCTCAAATGGATGCCCTTGCAGGCAAGTCCTAGTTTTGTTTTGTTTTTTCCCTACA TGTCAACTTAAATTAAAGTCTATACTGCTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC005583
Insert Size	1005 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005583, AAH05583
RefSeq Size	1179 bp
RefSeq ORF	1005 bp
Locus ID	13349
Gene Summary	Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Has a promiscuous chemokine-binding profile, interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1 and TARC. May regulate chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms: when expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes; when expressed in erythrocytes, serves as blood reservoir of cognate chemokines but also as a chemokine sink, buffering potential surges in plasma chemokine levels (By similarity).[UniProtKB/Swiss-Prot Function]

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