Cyp2e1 (BC013451) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cyp2e
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC013451 GTGGAGCCCCATCGGCACCATGGCGGTTCTTGGCATCACCGTTGCCTTGCTTGTCTGGATCGCCACCCTC CTCCTCGTATCCATCTGGAAACAGATCTATAGAAGTTGGAACCTGCCCCCAGGACCTTTCCCAATTCCTT TCTTTGGAAACATTTTTCAGCTGGATTTGAAGGATATTCCCAAGTCTTTAACCAAGTTGGCAAAGCGCTT CGGGCCAGTGTTCACACTGCACCTGGGTCAGAGGCGCATCGTGGTCCTGCATGGCTACAAGGCTGTCAAG GAGGTGCTACTGAACCACAAGAATGAGTTCTCTGGCCGAGGGGACATTCCTGTGTTCCAGGAGTACAAGA ACAAGGGGATTATTTTCAATAATGGACCCACATGGAAGGACGTGCGGAGGTTTTCCCTAAGTATCCTCCG TGACTGGGGAATGGGGAAACAGGGTAATGAGGCCCGCATCCAAAGAGAGGCACACTTCCTGGTGGAGGAG CTCAAAAAGACCAAAGGCCAGCCTTTTGACCCTACCTTTCTGATTGGCTGTGCACCCTGCAATGTCATTG CGGATATTCTCTTCAACAAACGCTTCGATTACGATGACAAGAAGTGTCTGGAGCTCATGAGTTTGTTCAA TGAAAACTTCTACCTGCTGAGTACTCCCTGGATCCAGGCTTACAATTACTTTTCGGATTATCTACAATAT CTACCTGGAAGCCACAGAAAAGTCATGAAAAATGTGTCTGAAATAAGACAGTACACACTTGGAAAAGCCA AGGAACACCTTAAGTCACTGGACATCAACTGCCCCCGGGATGTGACTGACTGTCTCCTCATAGAGATGGA GAAGGAAAAACACAGCCAAGAACCCATGTACACAATGGAAAATATTTCTGTGACTTTGGCCGACCTGTTC TTTGCAGGAACAGAGACCACCAGCACAACTCTGAGATATGGGCTCCTGATTCTCATGAAATACCCAGAAA TTGAAGAGAAACTTCATGAAGAAATTGACAGGGTTATTGGGCCAAGCCGTGCCCCTGCAGTCCGAGACAG GATGAATATGCCCTACATGGACGCTGTAGTGCATGAGATTCAGAGATTCATCAACCTCGTCCCTTCCAAC CTGCCCCACGAAGCAACCCGAGACACCGTGTTCCGAGGATATGTCATCCCCAAGGGTACAGTTGTAATTC CAACTCTGGACTCCCTTTTATTTGACAACTATGAGTTTCCAGATCCAGAGACATTTAAACCTGAGCATTT TCTGAATGAAAATGGGAAGTTCAAGTACAGTGACTATTTCAAGGCGTTTTCTGCAGGAAAGCGCGTGTGT GTTGGAGAAGGCCTGGCCCGCATGGAACTGTTTCTGCTTTTGTCTGCTATTCTGCAGCATTTTAATCTGA AGTCTCTGGTTGACCCTAAGGATATCGACCTCAGCCCTGTTACAATTGGCTTTGGCAGTATCCCACGCGA ATTTAAACTCTGTGTCATTCCTCGTTCATGAGACCTGCTGCCCATCATTATCCCTTACAAAATGACTGTT TAAAAAAATGCCAAGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC CGGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCTGTATCGAGATCTGACTCTGTCTTCTG GAGTGTCTGAAGACAGCTACAGTGTACTTACATATAATAAATAAATAAGTCTTTAAAAAAAAAAAAAAAA AAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC013451
Insert Size	1482 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC013451, AAH13451
RefSeq Size	1759 bp
RefSeq ORF	1482 bp
Locus ID	13106
Gene Summary	A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.