Chchd4 (BC019405) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2410012P20Rik; 2810014D17Rik; AI838740
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC019405 GGCGAGGGAGGTCACGGCGTGAAGGTGTATCCGCTCCAGCCGCGCCCGCTGCCTCTGCGAGCTGCAGAGG ACTGTCGGGAACAACCATGTCCTACTGCCGGCAGGAAGGGAAGGATCGGATCATATTTGTGACCAAAGAA GACCATGAAACTCCTAGCAGTGCCGAGCTGGTGGCTGATGACCCCAATGATCCCTATGAGGAGCACGGGT TGATACTGCCTAACGGAGATATTAACTGGAATTGTCCATGTCTTGGGGGAATGGCCAGCGGGCCCTGTGG GGAGCAGTTCAAGTCTGCCTTCTCCTGCTTCCACTACAGCACAGAGGATATCAAGGGATCAGACTGTATA GACCAGTTCCGGGCCATGCAAGAGTGCATGCAGAAGTACCCGGACCTCTATCCCCAAGACGAGGAGGAGG AAGAGGAGGCAAAGCCAGTGGAGCCGGTGGAGGAGACAGCTGACACTAAGGTCTCTGCAGCCAAAGAGCA GGGGACAAGCTCTTGAAGGCCACATGGCTGCCACTCACCATGGGACTTTCTGTACAATGCCTTTTGGTCA CCTTTGTGAAAGTTCTTTCCCTCTGTGCACTGTAATATACAAAATAACTTATTTTAATGATCAGGGGTCT TGGCCGTATACACTGAAGAAAAGGGCGTGTGTGCACCTGTGTCCTGCATAAACCTGTCCTTAAACATTAG TCCACTCCTGGATGTACTACTCTGTGATTGCCCTGGATTTTGCCACTTTGAAATAATGTGTTGAGACTTC TCAGCAACCAAAAGTTGTTACTCAGGAAAGTTTATCGTGAGAAAGCTGACCTATTCTGATCCATTTTGGT ATCCCTTTTGGTAGATTTTACACCTCTTTTGGAAATTAAGTAAATAGATGCCAAAACTCCTTTAAGAGTG GGTCCATTCCTACTGGAAGAGGCTGGTGGTCAGGTGCTTGCTGGAGTACATGGGGCTGCTGCCTCGTTCC CGACAGCTAATTGTCTGTTTGCTGTCCAGGCTCCAGTGCCTACAGCTGCAGGGTGGCATTGGATCCTGTG ACTCCCGGTCCTTCCTAAGTGAAAGGATGGGCTGGGTTTGCAGTTTATTGTTTTGCTGTTCTAAGAGCCC TGGTTTGGGGCCCATGTTCACTCAGTTGTGATATTGAGGTGGGGACCTAGCTGCTTCCAGAGCAGAAGCC AACAGAGGAATGTCTGAATGAAGTACTCAATTAAAAAGAAACAATTTCTATCTGAAAAAAAAAAAAAAAA A
Restriction Sites	EcoRI-NotI
ACCN	BC019405
Insert Size	420 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC019405, AAH19405
RefSeq Size	1261 bp
RefSeq ORF	420 bp
Locus ID	72170
Gene Summary	Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17, COX19 and MICU1. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system. Mediates formation of disulfide bond in MICU1 in the IMS, promoting formation of the MICU1-MICU2 heterodimer that regulates mitochondrial calcium uptake.[UniProtKB/Swiss-Prot Function]

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