Nkd1 (BC034838) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2810434J10Rik; 9030215G15Rik; Nkd
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC034838 CGACGGCGGCAGGAGCGCGTCCCGGCGCCGCCTAAGGCTGCGCTCGGCGCGCGGACTGTGAGGAGGAGGC GAGCGAGGCTGGCGCGGGGTCGGCGGCCGGACGCATGGCTTAGGACGCTCCGCCGCCGCGCCCCAGCATG GGGAAACTTCACTCGAAGCCGGCCGCCGTGTGCAAGCGCAGGGAGAGCCCGGAAGGTGACAGCTTTGCTG TAAGCGCTGCTTGGGCAAGGAAAGGCATCGAGGAGTGGATCGGGAGGCAGCGCTGTCCAGGCAGCGTCTC AGGACCCCGTCAGCTGAGATTGGCAGGCACTGTTGGTCGAGGCACTCGGGAACTCGTGGGTGACACTTCT AGAGAGGCTCTCGGTGAGGAGGACGAGGACGACTTCCCCCTAGAAGTGGCCCTGCCGCCTGAGAAGATTG ACAGCCTAGGTAGTGGAGATGAGAAGAGAATGGAGAGACTGAGCGAACCTGGCCAGGCCTCCAAGAAGCA GCTCAAGTTTGAAGAGCTGCAGTGTGATGTCTCTGTGGAGGAGGACAGCCGGCAAGAGTGGACTTTCACT CTATATGACTTCGACAACAATGGCAAAGTGACCCGTGAGGACATTACCAGCTTGCTGCATACCATCTATG AAGTGGTTGACTCCTCTGTGAACCATTCCCCCACATCAAGCAAGACACTGCGGGTGAAGCTCACCGTGGC TCCTGACGGGAGCCAGAGTAAGAGGAGCGTCCTTTTCAACCATACCGATCTGCAGAGCACAAGGCCCCGA GCAGACACCAAACCCGCTGAGGAGCTGCGTGGCTGGGAGAAGAAGCAGCGAGCCCCACTCAGGTTCCAGG GTGACAGCCACCTGGAGCAGCCAGACTGCTACCACCATTGCGTGGATGAGAACATTGAGAGGAGAAACCA CTACCTAGACCTGGCGGGGATAGAGAACTACACGTCTCAGTTTGGACCGGGATCCCCTTCGGTGGCCCAG AAGTCAGAGCTGCCCCCTCGAATCTCCAACCCCACTCGCTCTCGCTCCCACGAGCCAGAAGCTGCCCACG TCCCACACCGGAGGCCCCAAGGTGTGGACCCAGGCTCCTTCCACCTCCTTGACACCCCATTTGCCAAGGC ATCAGAGCTCCAGCAACGGCTCCGGGGCACTCAGGATGGGAGCAAGCACTTTGTGAGGTCCCCCAAGGCC CAGGGCAAGAACATGGGTATGGGCCACGGGGCCAGAGGTGCAAGAAGCAAGCCTCCACTGGTACCCACCA CCCATACTGTCTCCCCCTCTGCCCATCTGGCCACCAGCCCAGCCCTTCTCCCCACCCTGGCACCCCTGGG GCACAAGAAACACAAGCATCGAGCCAAGGAGAGCCAGGCGAGCTGCCGGGGCCTGCAGGGCCCCCTGGCT GCAGGAGGCTCCACCGTCATGGGGCGGGAGCAGGTGAGGGAGCTGCCTGCCGTGGTGGTGTACGAGAGCC AGGCTGGGCAGGCCGTCCAGAGACACGAACACCATCACCACCACGAACATCACCACCATTACCACCACTT CTATCAGCCCTAGACCCCAGCAGGCTGCCACGGGAAGGACCCAGCCCACACCCTAAGGCATTATTATTCT ATTAATTATTGTTATTATGGCAATTATTGTTATTAATAATTATTGTTACTCCACTAATATTTAGCCAGCC TTCATGTAGAAGACACATGGAAACACAGAAGTAAACTTTTATTTATAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC034838
Insert Size	1416 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC034838, AAH34838
RefSeq Size	1765 bp
RefSeq ORF	1416 bp
Locus ID	93960
Gene Summary	Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity. Required for spermatogenesis.[UniProtKB/Swiss-Prot Function]

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