Nkd1 (BC034838) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	2810434J10Rik; 9030215G15Rik; Nkd
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207531 representing BC034838 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAACTTCACTCGAAGCCGGCCGCCGTGTGCAAGCGCAGGGAGAGCCCGGAAGGTGACAGCTTTG CTGTAAGCGCTGCTTGGGCAAGGAAAGGCATCGAGGAGTGGATCGGGAGGCAGCGCTGTCCAGGCAGCGT CTCAGGACCCCGTCAGCTGAGATTGGCAGGCACTGTTGGTCGAGGCACTCGGGAACTCGTGGGTGACACT TCTAGAGAGGCTCTCGGTGAGGAGGACGAGGACGACTTCCCCCTAGAAGTGGCCCTGCCGCCTGAGAAGA TTGACAGCCTAGGTAGTGGAGATGAGAAGAGAATGGAGAGACTGAGCGAACCTGGCCAGGCCTCCAAGAA GCAGCTCAAGTTTGAAGAGCTGCAGTGTGATGTCTCTGTGGAGGAGGACAGCCGGCAAGAGTGGACTTTC ACTCTATATGACTTCGACAACAATGGCAAAGTGACCCGTGAGGACATTACCAGCTTGCTGCATACCATCT ATGAAGTGGTTGACTCCTCTGTGAACCATTCCCCCACATCAAGCAAGACACTGCGGGTGAAGCTCACCGT GGCTCCTGACGGGAGCCAGAGTAAGAGGAGCGTCCTTTTCAACCATACCGATCTGCAGAGCACAAGGCCC CGAGCAGACACCAAACCCGCTGAGGAGCTGCGTGGCTGGGAGAAGAAGCAGCGAGCCCCACTCAGGTTCC AGGGTGACAGCCACCTGGAGCAGCCAGACTGCTACCACCATTGCGTGGATGAGAACATTGAGAGGAGAAA CCACTACCTAGACCTGGCGGGGATAGAGAACTACACGTCTCAGTTTGGACCGGGATCCCCTTCGGTGGCC CAGAAGTCAGAGCTGCCCCCTCGAATCTCCAACCCCACTCGCTCTCGCTCCCACGAGCCAGAAGCTGCCC ACGTCCCACACCGGAGGCCCCAAGGTGTGGACCCAGGCTCCTTCCACCTCCTTGACACCCCATTTGCCAA GGCATCAGAGCTCCAGCAACGGCTCCGGGGCACTCAGGATGGGAGCAAGCACTTTGTGAGGTCCCCCAAG GCCCAGGGCAAGAACATGGGTATGGGCCACGGGGCCAGAGGTGCAAGAAGCAAGCCTCCACTGGTACCCA CCACCCATACTGTCTCCCCCTCTGCCCATCTGGCCACCAGCCCAGCCCTTCTCCCCACCCTGGCACCCCT GGGGCACAAGAAACACAAGCATCGAGCCAAGGAGAGCCAGGCGAGCTGCCGGGGCCTGCAGGGCCCCCTG GCTGCAGGAGGCTCCACCGTCATGGGGCGGGAGCAGGTGAGGGAGCTGCCTGCCGTGGTGGTGTACGAGA GCCAGGCTGGGCAGGCCGTCCAGAGACACGAACACCATCACCACCACGAACATCACCACCATTACCACCA CTTCTATCAGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207531 representing BC034838 Red=Cloning site Green=Tags(s) MGKLHSKPAAVCKRRESPEGDSFAVSAAWARKGIEEWIGRQRCPGSVSGPRQLRLAGTVGRGTRELVGDT SREALGEEDEDDFPLEVALPPEKIDSLGSGDEKRMERLSEPGQASKKQLKFEELQCDVSVEEDSRQEWTF TLYDFDNNGKVTREDITSLLHTIYEVVDSSVNHSPTSSKTLRVKLTVAPDGSQSKRSVLFNHTDLQSTRP RADTKPAEELRGWEKKQRAPLRFQGDSHLEQPDCYHHCVDENIERRNHYLDLAGIENYTSQFGPGSPSVA QKSELPPRISNPTRSRSHEPEAAHVPHRRPQGVDPGSFHLLDTPFAKASELQQRLRGTQDGSKHFVRSPK AQGKNMGMGHGARGARSKPPLVPTTHTVSPSAHLATSPALLPTLAPLGHKKHKHRAKESQASCRGLQGPL AAGGSTVMGREQVRELPAVVVYESQAGQAVQRHEHHHHHEHHHHYHHFYQP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC034838
ORF Size	1415 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC034838, AAH34838
RefSeq Size	1765 bp
RefSeq ORF	1415 bp
Locus ID	93960
Gene Summary	Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity. Required for spermatogenesis.[UniProtKB/Swiss-Prot Function]

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