United States
Germany
Japan
United Kingdom
China
Gfap (NM_010277) Mouse Untagged Clone
CAT＃: MC208540
Gfap (untagged) - Mouse glial fibrillary acidic protein (Gfap), transcript variant 2, (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI836096 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC208540 representing NM_010277
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGAGACGCATCACCTCTGCGCGCCGCTCCTATGCCTCCGAGACGGTGGTCAGGGGCCTCGGTC CTAGTCGACAACTGGGTACCATGCCACGCTTCTCCTTGTCTCGAATGACTCCTCCACTCCCTGCCAGGGT GGACTTCTCCCTGGCCGGGGCGCTCAATGCTGGCTTCAAGGAGACACGGGCGAGCGAGCGTGCAGAGATG ATGGAGCTCAATGACCGCTTTGCTAGCTACATCGAGAAGGTCCGCTTCCTGGAACAGCAAAACAAGGCGC TGGCAGCTGAACTGAACCAGCTTCGAGCCAAGGAGCCCACCAAACTGGCTGATGTCTACCAGGCGGAGCT TCGGGAGCTGCGGCTGCGGCTGGACCAGCTTACGGCCAACAGTGCCCGGCTGGAGGTGGAGAGGGACAAC TTTGCACAGGACCTCGGCACCCTGAGGCAGAAGCTCCAAGATGAAACCAACCTGAGGCTGGAGGCAGAGA ACAACCTGGCTGCGTATAGACAGGAGGCAGATGAAGCCACCCTGGCTCGTGTGGATTTGGAGAGAAAGGT TGAATCGCTGGAGGAGGAGATCCAGTTCTTAAGGAAGATCTATGAGGAGGAAGTTCGAGAACTCCGGGAG CAGCTGGCCCAACAGCAGGTCCACGTGGAGATGGATGTGGCCAAGCCAGACCTCACAGCGGCCCTGAGAG AGATTCGCACTCAATACGAGGCAGTGGCCACCAGTAACATGCAAGAGACAGAGGAGTGGTATCGGTCTAA GTTTGCAGACCTCACAGACGCTGCGTCCCGCAACGCAGAGCTGCTCCGCCAAGCCAAGCACGAAGCTAAC GACTATCGCCGCCAACTGCAGGCCTTGACCTGCGATCTGGAGTCCCTGCGCGGCACGAACGAGTCCCTAG AGCGGCAAATGCGCGAACAGGAAGAGCGCCATGCGCGGGAGTCGGCCAGTTACCAGGAGGCACTTGCTCG GCTGGAGGAGGAGGGCCAAAGCCTCAAGGAGGAGATGGCCCGCCACCTGCAGGAGTACCAGGATCTACTC AACGTTAAGCTAGCCCTGGACATCGAGATCGCCACCTACAGGAAATTGCTGGAGGGCGAAGAAAACCGCA TCACCATTCCTGTACAGACTTTCTCCAACCTCCAGATCCGAGAAACCAGCCTGGACACCAAATCCGTGTC AGAAGGCCACCTCAAGAGGAACATCGTGGTAAAGACTGTGGAGATGCGGGATGGTGAGGTCATTAAGGAC TCGAAGCAGGAGCACAAGGACGTGGTGATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010277 |
| Insert Size | 1293 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010277.3, NP_034407.2 |
| RefSeq Size | 2733 bp |
| RefSeq ORF | 1293 bp |
| Locus ID | 14580 |
| UniProt ID | P03995 |
| Gene Summary | GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1, which results in a protein (isoform 2) with a shorter and distinct C-terminus when compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226731 | Gfap (tGFP-tagged) - Mouse glial fibrillary acidic protein (Gfap) transcript variant 2, (10ug) |
CNY 3,140.00 |
|
| MR226731 | Gfap (Myc-DDK-tagged) - Mouse glial fibrillary acidic protein (Gfap), transcript variant 2 |
CNY 3,656.00 |
|
| MR226731L3 | Lenti ORF clone of Gfap (Myc-DDK-tagged) - Mouse glial fibrillary acidic protein (Gfap), transcript variant 2 |
CNY 4,750.00 |
|
| MR226731L4 | Lenti ORF clone of Gfap (mGFP-tagged) - Mouse glial fibrillary acidic protein (Gfap), transcript variant 2 |
CNY 4,750.00 |
