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Nck1 (NM_010878) Mouse Untagged Clone
CAT＃: MC209014
Nck1 (untagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 1 (Nck1), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 6330586M15Rik; D230010O13Rik; Nck; Nck-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209014 representing NM_010878
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAAGAAGTGGTGGTGGTGGCCAAATTTGATTATGTGGCACAGCAGGAACAAGAGCTGGATATCA AGAAGAATGAGCGATTATGGCTCCTGGATGACTCTAAATCCTGGTGGCGAGTTCGAAATTCCATGAATAA AACAGGTTTTGTCCCTTCTAACTATGTGGAAAGAAAAAACAGTGCTCGGAAAGCATCTATTGTTAAAAAC CTGAAGGACACCTTAGGTATTGGAAAAGTGAAAAGAAAACCCAGTGTTCCAGATACTGCATCTCCTGCTG ATGATAGCTTTGTTGATCCAGGAGAACGTCTCTATGACCTTAACATGCCTGCTTTTGTGAAATTTAACTA CATGGCTGAGAGAGAGGATGAGTTGTCATTGATAAAAGGGACCAAGGTGATCGTCATGGAGAAATGCAGT GATGGATGGTGGCGTGGCAGCTACAACGGACAAATTGGATGGTTTCCTTCAAACTATGTAACTGAAGAAG GTGACAGTCCTTTGGGTGATCATGTAGGTTCTCTGTCAGAGAAATTAGCAGCAGTTGTCAATAACCTAAA TACGGGTCAAGTATTGCATGTTGTACAGGCTCTTTACCCGTTTAGCTCATCCAATGATGAAGAACTCAAT TTTGAGAAAGGCGATGTAATGGATGTTATTGAAAAGCCGGAAAATGACCCAGAGTGGTGGAAATGCAGGA AAATCAATGGCATGGTTGGCCTGGTGCCAAAAAACTACGTTACCATTATGCAAAACAATCCATTAACCTC AGGTTTGGAACCATCTCCTCCACAATGTGATTACATTAGGCCTTCACTCACTGGGAAGTTTGCTGGCAAT CCTTGGTATTATGGCAAAGTCACCAGGCACCAGGCAGAAATGGCATTAAATGAAAGAGGGCATGAAGGAG ACTTCCTCATTCGTGACAGTGAATCTTCGCCAAATGATTTCTCAGTATCACTAAAAGCACAAGGGAAAAA CAAGCATTTTAAAGTCCAGCTGAAAGAGACTGTTTACTGCATTGGGCAGCGGAAATTCAGCACCATGGAG GAACTTGTAGAACATTACAAAAAGGCACCGATCTTTACAAGTGAGCAAGGAGAAAAATTATATCTCGTCA AGCATTTGTCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010878 |
| Insert Size | 1134 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010878.3, NP_035008.2 |
| RefSeq Size | 2467 bp |
| RefSeq ORF | 1134 bp |
| Locus ID | 17973 |
| UniProt ID | Q99M51 |
| Gene Summary | Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205875 | Nck1 (tGFP-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 1 (Nck1) |
CNY 3,140.00 |
|
| MR205875 | Nck1 (Myc-DDK-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 1 (Nck1) |
CNY 3,656.00 |
|
| MR205875L3 | Lenti ORF clone of Nck1 (Myc-DDK-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 1 (Nck1) |
CNY 4,750.00 |
|
| MR205875L4 | Lenti ORF clone of Nck1 (mGFP-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 1 (Nck1) |
CNY 4,750.00 |
