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Wnt3a (NM_009522) Mouse Untagged Clone
CAT＃: MC209652
Wnt3a (untagged) - Mouse wingless-related MMTV integration site 3A (Wnt3a), (10ug)
CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | vt; Wnt-3a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209652 representing NM_009522
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCTCTCGGATACCTCTTAGTGCTCTGCAGCCTGAAGCAGGCTCTGGGCAGCTACCCGATCTGGT GGTCCTTGGCTGTGGGACCCCAGTACTCCTCTCTGAGCACTCAGCCCATTCTCTGTGCCAGCATCCCAGG CCTGGTACCGAAGCAGCTGCGCTTCTGCAGGAACTACGTGGAGATCATGCCCAGCGTGGCTGAGGGTGTC AAAGCGGGCATCCAGGAGTGCCAGCACCAGTTCCGAGGCCGGCGTTGGAACTGCACCACCGTCAGCAACA GCCTGGCCATCTTTGGCCCTGTTCTGGACAAAGCCACCCGGGAGTCAGCCTTTGTCCATGCCATCGCCTC CGCTGGAGTAGCTTTCGCAGTGACACGCTCCTGTGCAGAGGGATCAGCTGCTATCTGTGGGTGCAGCAGC CGCCTCCAGGGCTCCCCAGGCGAGGGCTGGAAGTGGGGCGGCTGTAGTGAGGACATTGAATTTGGAGGAA TGGTCTCTCGGGAGTTTGCCGATGCCAGGGAGAACCGGCCGGATGCCCGCTCTGCCATGAACCGTCACAA CAATGAGGCTGGGCGCCAGGCCATCGCCAGTCACATGCACCTCAAGTGCAAATGCCACGGGCTATCTGGC AGCTGTGAAGTGAAGACCTGCTGGTGGTCGCAGCCGGACTTCCGCACCATCGGGGATTTCCTCAAGGACA AGTATGACAGTGCCTCGGAGATGGTGGTAGAGAAACACCGAGAGTCTCGTGGCTGGGTGGAGACCCTGAG GCCACGTTACACGTACTTCAAGGTGCCGACAGAACGCGACCTGGTCTACTACGAGGCCTCACCCAACTTC TGCGAACCTAACCCCGAAACCGGCTCCTTCGGGACGCGTGACCGCACCTGCAATGTGAGCTCGCATGGCA TAGATGGGTGCGACCTGTTGTGCTGCGGGCGCGGGCATAACGCGCGCACTGAGCGACGGAGGGAGAAATG CCACTGTGTTTTCCATTGGTGCTGCTACGTCAGCTGCCAGGAGTGCACACGTGTCTATGACGTGCACACC TGCAAGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-RsrII |
| ACCN | NM_009522 |
| Insert Size | 1059 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009522.2, NP_033548.1 |
| RefSeq Size | 2791 bp |
| RefSeq ORF | 1059 bp |
| Locus ID | 22416 |
| UniProt ID | P27467 |
| Gene Summary | Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:26902720). Required for normal embryonic mesoderm development and formation of caudal somites (PubMed:8299937). Required for normal morphogenesis of the developing neural tube (PubMed:8299937). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed:26902720).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Activation of the Wnt/ß-catenin pathway represses the transcription of the ß-amyloid precursor protein cleaving enzyme (BACE1) via binding of T-cell factor-4 to BACE1 promoter
,Parr, C;Mirzaei, N;Christian, M;Sastre, M;,
FASEB J.
,PubMed ID 25384422
[Wnt3a]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227165 | Wnt3a (tGFP-tagged) - Mouse wingless-related MMTV integration site 3A (Wnt3a), (10ug) |
CNY 7,088.00 |
|
| MR227165 | Wnt3a (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 3A (Wnt3a) |
CNY 5,488.00 |
|
| MR227165L1 | Lenti ORF clone of Wnt3a (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 3A (Wnt3a) |
CNY 7,888.00 |
|
| MR227165L2 | Lenti ORF clone of Wnt3a (mGFP-tagged) - Mouse wingless-related MMTV integration site 3A (Wnt3a) |
CNY 5,890.00 |
|
| MR227165L3 | Lenti ORF clone of Wnt3a (Myc-DDK-tagged) - Mouse wingless-related MMTV integration site 3A (Wnt3a) |
CNY 5,890.00 |
|
| MR227165L4 | Lenti ORF clone of Wnt3a (mGFP-tagged) - Mouse wingless-related MMTV integration site 3A (Wnt3a) |
CNY 7,888.00 |
