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Mapk10 (NM_009158) Mouse Untagged Clone
CAT＃: MC209721
Mapk10 (untagged) - Mouse mitogen-activated protein kinase 10 (Mapk10), transcript variant 1, (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | C230008H04Rik; JNK; JNK3; JNK3B1; JNK3B2; p54bSAPK; p493F1; p493F12; SAPK(beta); Ser; Serk2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC209721 representing NM_009158
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTCCATTTCTTATACTACTGCAGTGAACCAACCTTGGATGTGAAAATTGCCTTTTGTCAGGGAT TCGATAAACACGTGGATGTGTCATCTATTGCCAAACATTACAACATGAGCAAAAGCAAGGTGGACAACCA GTTCTACAGTGTGGAAGTGGGGGACTCAACCTTCACCGTTCTTAAGCGCTACCAGAACCTGAAGCCAATT GGCTCTGGGGCTCAGGGAATAGTCTGTGCTGCGTACGACGCTGTCCTTGACAGAAATGTGGCCATTAAGA AGCTCAGCAGACCCTTCCAGAACCAAACTCACGCCAAGAGGGCTTACCGGGAGCTGGTCCTCATGAAGTG TGTGAACCATAAAAACATTATTAGCTTATTAAATGTTTTTACACCCCAGAAAACACTGGAGGAGTTCCAA GATGTCTACTTAGTGATGGAACTGATGGACGCCAACCTGTGTCAGGTGATTCAGATGGAGCTGGACCACG AGCGGATGTCTTACTTGCTGTACCAGATGCTGTGTGGCATCAAGCACCTCCACTCCGCTGGGATCATCCA CAGGGACTTAAAACCCAGTAACATTGTAGTCAAGTCTGATTGCACACTGAAAATCCTCGACTTCGGACTG GCCAGGACAGCGGGTACAAGCTTCATGATGACTCCGTATGTGGTGACGCGATATTACAGAGCCCCTGAGG TCATCCTGGGCATGGGCTACAAGGAGAACGTGGACATATGGTCTGTGGGATGCATCATGGGAGAAATGGT TCGCCACAAAATCCTCTTTCCCGGAAGGGACTATATTGACCAGTGGAACAAAGTCATCGAGCAGCTAGGA ACTCCGTGTCCAGAGTTCATGAAGAAATTGCAGCCCACAGTCAGAAACTACGTGGAGAATCGGCCCAAGT ACGCAGGACTCACCTTCCCCAAGCTCTTTCCAGATTCCCTCTTCCCAGCGGATTCTGAGCACAATAAACT TAAAGCCAGCCAAGCCAGGGATTTGTTGTCTAAGATGTTAGTGATTGACCCAGCGAAGAGGATATCGGTG GACGACGCACTGCAGCATCCGTACATCAACGTTTGGTACGACCCGGCTGAAGTGGAGGCGCCTCCGCCTC AGATATATGATAAGCAGCTGGATGAAAGGGAGCACACCATCGAAGAATGGAAAGAACTTATCTACAAGGA GGTAATGAACTCAGAAGAGAAGACTAAGAATGGCGTAGTCAAAGGCCAGCCCTCGCCTTCAGCACAGGTG CAGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009158 |
| Insert Size | 1269 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009158.3, NP_033184.2 |
| RefSeq Size | 6604 bp |
| RefSeq ORF | 1269 bp |
| Locus ID | 26414 |
| Gene Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017] Transcript Variant: This variant (2) uses an alternate acceptor splice site at the 3' terminal exon, which causes a frameshift compared to variant 1. The resulting isoform (2, also know as JNK3 alpha1) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206714 | Mapk10 (tGFP-tagged) - Mouse mitogen activated protein kinase 10 (Mapk10), transcript variant 1 |
CNY 5,130.00 |
|
| MG226312 | Mapk10 (tGFP-tagged) - Mouse mitogen-activated protein kinase 10 (Mapk10) transcript variant 1, (10ug) |
CNY 7,088.00 |
|
| MR226312 | Mapk10 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 10 (Mapk10), transcript variant 1 |
CNY 5,488.00 |
|
| MR226312L3 | Lenti ORF clone of Mapk10 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 10 (Mapk10), transcript variant 1 |
CNY 5,230.00 |
|
| MR226312L4 | Lenti ORF clone of Mapk10 (mGFP-tagged) - Mouse mitogen-activated protein kinase 10 (Mapk10), transcript variant 1 |
CNY 5,230.00 |
