Rgs20 (NM_021374) Mouse Untagged Clone

CAT#: MC210158

Rgs20 (untagged) - Mouse regulator of G-protein signaling 20 (Rgs20), transcript variant 2, (10ug)



  "NM_021374" in other vectors (4)

CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2900073E09Rik; Rgsz1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC210158 representing NM_021374
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCACGGCCAACGGAGGCCCGAGGGCCAGAGCTTCCCCTTCGGCTAGCCCAGCGGACCCTGGCCTGC
CTGAGGGATCTGAGCGGACGGAAATGCGGATGCGGCAGATGTGCGGCGGAAGTGAGACCCAGGGCCCTGC
CCCAAGCCAGCAGGGTGGCCGGGGCTCCAACGCCTGCTGCTTCTGTTGGTGCTGCTGCTGCACCTGTTCT
TGCCTCACTGTCAGAAACCAAGAAGACCAGAGACCCCAAAGAGCCTCCCATGAAATCAGAACAGACATTC
CAGCATGTGAAGAAAGCCCCACGCCTACTCTGGAAGAAGTCTGTGCCTGGGCCCAGTCTTTTGATAACCT
GATGGTCACTCCAGCCGGAAGAAATGCATTCCGGGAATTTCTTCGAACAGAATTCAGTGAAGAAAACATG
CTCTTCTGGATGGCCTGTGAAGAACTGAAAAGGGAAGCTAATAAAAGCACTATTGAGGAGAAAGCAAGGA
TAATCTATGAGGACTATATTTCTATTCTCTCTCCTAAAGAGGTCAGCTTGGACTCCCGAGTACGAGAGGT
TATCAACAGAAACATGGTGGATCCATCCCAGCACATATTCGATGACGCTCAACTGCAGATCTACACTCTA
ATGCACAGAGACTCCTATCCCCGCTTCATGAACTCCACAGTCTATAAGGATTTGCTTACGTCCTTAGCTG
AGAAAACAGTTGAAGCATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_021374
Insert Size 720 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021374.5, NP_067349.2
RefSeq Size 1778 bp
RefSeq ORF 720 bp
Locus ID 58175
UniProt ID Q9QZB1
Gene Summary Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The resulting isoform (2) is shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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