Spdya (NM_029254) Mouse Untagged Clone

CAT#: MC211111

Spdya (untagged) - Mouse speedy homolog A (Xenopus laevis) (Spdya), transcript variant 1, (10ug)



  "NM_029254" in other vectors (4)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4921517J08Rik; 4930548B21Rik; GS4; MLZ-465; Spdy1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC211111 representing NM_029254
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCATAATCAGATGTATTGTGAGACACCACCTACTGTCACTATTCATGTAAAATCAGGCTCAAATA
GGTCACATCAAACCAGAAAACCTATTAGTCTGAAACGTCCTATTCTTAAAGATAGTTGGGAAGCATCTGA
AAACAATGCTCAGAATAACAAATCTAAGCGGCCCAGAGGGCCTTGTCTAATCATACAGCGCCAGGAAATG
ACTGCTTTCTTTAAATTATTTGATGATGATTTAATTCAAGATTTCTTGTGGATGGACTGCTGCTGCAAGA
TTGCAGACAAGTATCTTTTGGCTATGACCTTTGTTTATTTCAAGAGAGCTAAATTTACTATAAATGAGCA
TACCAGGATAAATTTCTTTATTGCTCTGTATCTGGCTAATACGGTTGAAGAAGATGAAGAAGAAGCCAAG
TATGAAATTTTTCCATGGGCTTTAGGGAAAAACTGGAGAAAACTGTTCCCTAATTTCTTAAAGTTAAGGG
ACCAACTCTGGGACAGAATTGACTATAGGGCTATTGTAAGCAGGCGATGCTGTGAAGAGGTCATGGCCAT
TGCGCCAACCCATTACATCTGGCAACGAGAGCGGTCTGTGCATCACAGTGGAGCTGTTAGGAACTACAAC
AGAGATGAGGTTCACCTGCCCAGGGGACCTAGTGCCACACCAGTGGATTGCTCACTGTGTGGTAAAAAAG
GAAGATACGTGAGACTGGGACTGTCTTCATCCTCATCTTCCTCCAGTGACACAGGAGAGCTAATGGAAAA
AGATTCTCAGGAACTACACAGTGCATTCTCAGTGGACACGGCAGGTGACCCTCCTCATACCTATTCTCAA
GTAGCCAATGACCATCAATCAAACAAAGAAAATGAAACTAATTTTGTGAAGAAAAACAAATCCGTGGAAT
GGTTTGCAGAGAGTGAAGAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_029254
Insert Size 933 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_029254.1, NP_083530.1
RefSeq Size 1891 bp
RefSeq ORF 933 bp
Locus ID 70891
UniProt ID Q5IBH7
Gene Summary Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2 (PubMed:15611625). Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation. Interferes with CDKN1B-mediated inhibition of CDK2. Mediates cell survival during the DNA damage process through activation of CDK2 (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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